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Application of oxidized high-density lipoprotein

A high-density lipoprotein, oxidized technology, applied in the application field of oxidized high-density lipoprotein, can solve problems such as unclear

Active Publication Date: 2020-05-19
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether oxidative modification of HDL occurs in CAVD patients and what role ox-HDL plays in the mineralization of AVICs remains unclear.

Method used

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  • Application of oxidized high-density lipoprotein
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  • Application of oxidized high-density lipoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1.1 Subject population

[0041] A total of 2674 patients with symptoms of myocardial ischemia were continuously collected in Shanghai Ruijin Hospital, and echocardiography and coronary angiography were performed on each patient. Among them, 168 patients were diagnosed as CAVD patients by echocardiography and Doppler blood flow imaging, and they were included in the CAVD group. The aortic valve in a patient with CAVD exhibited calcific degeneration and met the criteria for leaflet opacity with mild lesion thickening and leaflet stiffness (according to AHA guidelines). Each CAVD patient was age (±1 year) and gender-matched with a non-CAVD patient among the remaining patients, and entered into the non-CAVD group.

[0042] Patients with a history of rheumatic disease, bicuspid aortic valve malformation, moderate to severe aortic regurgitation, or inflammatory disease were not included in the study to avoid confounding factors. CAD was diagnosed when the stenosis of the ma...

Embodiment 2

[0069] 2.1 Preparation of HDL and ox-HDL

[0070] Fresh fasting plasma from 10 healthy normal participants was collected and mixed, and HDL (1.063–1.210g / mL) was separated by density gradient centrifugation. 5h; then the HDL obtained by centrifugation was placed in phosphate buffered saline (PBS, pH 7.4) for full dialysis; finally, the HDL after dialysis was filtered with a 0.22 μm pore size filter, and stored in the dark at 4°C until use (N-HDL ). The concentration of apoA-1 was determined by SDS-PAGE, and then the concentration of HDL was determined.

[0071] HDL was co-incubated with copper sulfate solution (20 μmol / L) prepared by PBS at 37°C for 24 hours, then EDTA was added to stop the oxidation reaction, and then dialyzed in PBS for 48 hours to obtain ox-HDL for later use. The degree of HDL oxidation was determined by TBARS kit, and the degree of HDL oxidation was expressed as nanomole amount of TBARS per mg of protein.

[0072] The TBARS value in ox-HDL was 25.3±5.2n...

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Abstract

The invention belongs to the field of medicine, and particularly relates to an application of oxidized high-density lipoprotein. The oxidized high-density lipoprotein is used as a target or a marker of calcified aortic valve diseases. The oxidized high-density lipoprotein and / or an oxidized high-density lipoprotein detection reagent can be used for preparing a diagnostic agent of the calcified aortic valve diseases, monitoring a concentration of the oxidized high-density lipoprotein and predicting occurrence and a progress of the calcified aortic valve diseases. An inhibitor or blocker of theoxidized high-density lipoprotein can also be used for preparing medicine for treating the calcified aortic valve diseases and treating the calcified aortic valve diseases. The oxidized high-density lipoprotein and / or the oxidized high-density lipoprotein detection reagent can also be used for preparing a screening agent of calcified aortic valve disease treatment medicine and screening the medicine for treating the calcified aortic valve diseases. The application of the oxidized high-density lipoprotein provides a new way and basis for clinical diagnosis and treatment of the calcified aorticvalve diseases.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of oxidized high-density lipoprotein. Background technique [0002] Calcific aortic valve disease (CAVD) is currently the most common heart valve disease worldwide and is characterized by progressive mineralization of the aortic valve. However, the specific cellular and molecular mechanisms of calcific aortic disease have not been fully understood. Similar to the pathogenic mechanism of atherosclerosis, increasing lipid infiltration and increasing oxidative stress levels can both promote the occurrence and development of CAVD. [0003] Aortic valve interstitial cells (AVICs) are the most abundant cell type in valve leaflets and play an important role in maintaining aortic valve function. In normal aortic valves, AVICs are quiescent fibroblast-like cells (qVICs); while a series of changes induced by mechanical stress and disease states (such as lipid infiltrat...

Claims

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Application Information

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IPC IPC(8): G01N33/92A61K45/00A61P9/00
CPCG01N33/92A61K45/00A61P9/00G01N2333/775G01N2800/329
Inventor 孙嘉腾杨克刘艳陈媛媛陈亚芬王燕萍毛静妍
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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