Method for measuring ABCC2 gene polymorphism

Abstract

The invention belongs to the field of molecular biological techniques and medical examination, and relates to a device for detecting drug transporter ABCC2 gene polymorphism, in particular to a method for detecting ABCC2 c. 1249 G>A and ABCC 2 3972>T gene polymorphism. After DNA of a sample is extracted, conventional PCR (polymerase chain reaction) amplification (containing fluorescent dye) is carried out, and a melting curve with high resolution ratio is used for analyzing genetic typing of the sample. The method is easy and convenient to operate, a sequence specificity probe is not required, the method is not limited by basic sites, meanwhile, cross contamination is avoided by closed tube operation, and the method has the features of high efficiency, quickness, sensitivity, low cost and high flux, is suitable for clinical conventional gene detection, and further provides a technical support for clinical individual rational drug use.

Description

technical field [0001] The invention belongs to the fields of molecular biology technology and medical examination, and relates to a detection method for the polymorphism of the drug transporter ABCC2 gene, in particular to a high-resolution melting curve analysis method for determining the polymorphism of the human ABCC2 gene, and in particular to a method for detecting the polymorphism of the human ABCC2 gene. A method for detecting the gene polymorphisms of ABCC2c.1249G>A and ABCC23972C>T by the resolution melting curve analysis technique. Background technique [0002] The prior art discloses that heredity is one of the important factors leading to individual differences in drug response. Therefore, using genetic testing to obtain genetic information of patients is of great significance for guiding individualized drug use, improving clinical efficacy and ensuring drug safety. In recent years, the number of drug-related genetic testing products approved by the FDA in...

AI Technical Summary

Problems solved by technology

The Taqman is a highly specific quantitative PCR technology, which has the advantages of fewer steps, less pollution, and is convenient for typing large samples; however, it has certain restrictions on primer design, and has certain requirements for sequences near SNPs, which are subject to mutation bases. Site and type limitations; the DNA direct sequencing method is the gold standard for mutation detection, but it is time-consuming, laborious, expensive and other defects, so it is not suitable for detection of a large number of samples

In addition to the above two commonly used methods, some gene products have appeared on the market. Roche’s NimbleGenAccuSNP genotyping chip, which uses chip technology to quickly type mutations at known sites, is one of the hot applications of gene chips at present. The gene chip is fast, accurate, and simple, and the experimental report is qualitative. Its disadvantage is that it is expensive, up to several hundred dollars, and it cannot process a large number of samples at the same time.

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