39results about How to "Help nurture" patented technology

The invention discloses application of an OsNPF5.11 gene to improvement in rice yield and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF5.11 gene encoding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Through constructing a rice OsNPF5.11 gene overexpressing plant and an OsNPF5.11 gene interference plant, the increase in the normal number of tillers of rice and the number of grouting grains per plant through improving the expression of the OsNPF5.11 gene can be discovered; therefore, the OsNPF5.11 gene can be used in rice breeding to improve the rice yield. The OsNPF5.11 gene has an important application value in aspects of elaborating the influence of nitrogen elements on plant growth and development process and improving the rice plant type.

The invention discloses a method for planting organic vegetable okra. The method mainly comprises the steps of seed processing, soil preparation and fertilization, seedling transplantation, field management and harvesting. During the middle term of the okra, intertillage and earthing up need performing in time, and the soil is topdressed with organic fertilizers containing amino acid and humic acid. During insect control, natural source levorotation terpilenol and S-S rosin insecticides are utilized, diseased leaves, plant residues and weeds are processed through an organic method, and the whole process is free of pollution caused by chemicals. The organic planting model of the okra provides a firm technical foundation for promoting large-scale and standardized production of the organic vegetable okra planting industry.

The invention discloses the application of the rice OsRHD1‑1 gene in the cultivation of rice male sterility lines. The present invention constructs a gene knockout vector by designing a knockout target of the OsRHD1-1 gene, and performs genetic transformation in rice, and finds that the loss of OsRHD1-1 gene function can lead to pollen sterility, which is helpful for a new type of male sterile line The development of hybrid breeding is facilitated.

The invention relates to an artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. The method comprises the following steps: (1) taking a fruiting body of mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils., and collecting fungus context issue blocks at the intersecting part of a fungus cap and a fungus stipe; (2) putting the fungus context tissue blocks in a culture plate, culturing the fungus context tissue blocks at the temperature of 25-28 DEG C until mycelia are germinated by the fungus context issue blocks, transferring the mycelia, and then cultivating the mycelia at the temperature of 25-28 DEG C until the mycelia grow to cover the culture plate to obtain purified mycelia; (3) selecting the purified mycelia, and inoculating the selected purified mycelia to an improved MMN liquid culture medium for fermental cultivation, thus obtaining a bacterium solution; (4) inoculating the bacterium solution to an enlarged cultivating material, and cultivating the purified mycelia at the temperature of 25-28 DEG C until the purified mycelia grow all over a cultivating container to obtain mycorrhizal fungi inoculant of Castanea henryi (Skam) Rehd. et Wils. The artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. is simple in operation; when mycorrhizal fungi cultivated and obtained through the artificial cultivating method is inoculated to seedlings of Castanea henryi Rehd. et Wils., the impregnating rate is high.

The invention provides a processing method of sumac seedling seeds. The method comprises the steps of storing sumac seeds in sand, soaking the seeds in saline solution, and cleaning; soaking the seeds in washing powder water, rubbing the surfaces of the seeds to remove wax, and putting the seeds into a woven bag; spraying warm water onto the seeds every other 2 hours until the seeds are expanded and the seed coats are softened; putting the seeds into a breathable bamboo frame or a plastic frame, and watering until the seeds crack and sprout. According to the processing method, the characteristics that the sumac seedling seeds have after-ripening embryo, and seed shells are firm, seed coats are compact and the waxiness is thick can be effectively broken, the cultivation of the high-quality sumac nursery stock can be facilitated, the germination rate of the sumac seeds reaches more than 50% after 5-6 days, the sumac seeds can be planted in the nursery garden land by broadcast sowing or hill-seeding in the seventh day, the germination rate reaches up to more than 95% within 15 days after sowing, and 60% of seeds can germinate within a week. According to the processing method, the germination period is short, more than half time can be shortened, the seeds can be processed in batches on a large scale, and the cost is low; furthermore, a chemical reagent with high harmfulness is eliminated, and the processing method has the characteristics of being safe, environmentally friendly and easy to operate.