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Method for planting organic vegetable okra
InactiveCN103931377AImprove securityEnsure safetyPlant protectionSeed coating/dressingNatural sourceSeed treatment
The invention discloses a method for planting organic vegetable okra. The method mainly comprises the steps of seed processing, soil preparation and fertilization, seedling transplantation, field management and harvesting. During the middle term of the okra, intertillage and earthing up need performing in time, and the soil is topdressed with organic fertilizers containing amino acid and humic acid. During insect control, natural source levorotation terpilenol and S-S rosin insecticides are utilized, diseased leaves, plant residues and weeds are processed through an organic method, and the whole process is free of pollution caused by chemicals. The organic planting model of the okra provides a firm technical foundation for promoting large-scale and standardized production of the organic vegetable okra planting industry.
Owner:GUIGANG HEAN HUIXING AGRI SCI & TECH
Application of nitrate radical transporter gene OsNRT1.8 in rice breeding
ActiveCN106337055AEnhanced tillering abilityIncrease productionPlant peptidesFermentationGrowth plantNitrogen
The invention discloses application of a nitrate radical transporter gene OsNRT1.8 in rice breeding and belongs to the field of plant genetic engineering. The protein encoding amino acid sequence of the gene OsNRT1.8 is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. By establishing rice gene OsNRT1.8 overexpression plant and a gene OsNRT1.8 interference plant, it is found that normal rice tiller number and spike number of each plant can be increased by improving gene OsNRT1.8 expression. Therefore, the gene OsNRT1.8 can be used for increasing rice yield in rice breeding. The gene OsNRT1.8 has an important application value on the aspects of elaboration about nitrogen influence on plant growth and development and improvement of rice plant type.
Owner:WUHAN INST OF BIOENG
Application of amino acid transporter gene OsAAP3 in rice seed selection
ActiveCN106518993AEnhanced tillering abilityIncrease the number of tillersHydrolasesPlant peptidesPlant genetic engineeringGenetic engineering
The invention discloses application of an amino acid transporter gene OsAAP3 in rice seed selection, and belongs to the technical field of genetic engineering. An amino acid sequence of encoded protein of the gene OsAAP3 is specified in SEQ ID NO.1, and a Cdna sequence is specified in SEQ ID NO.2. According to the application of the amino acid transporter gene OsAAP3 in rice seed selection, by constructing a rice plant which is interfered by the gene OsAAP3, and constructing a rice plant which is over-expressed by the gene OsAAP3, it is discovered that reduction of genetic expression of the gene OsAAP3 can increase tiller number of the rice and spike number in each rice plant, and therefore the gene OsAAP3 can be used in rice seed selection for increasing the rice yield. The gene OsAAP3 has important application value in expounding influence of amino acid transportation on growth and development process of the plant.
Owner:WUHAN INST OF BIOENG
Okra planting method
InactiveCN106069099AImprove disease resistanceStrong disease resistanceSuperphosphatesMagnesium fertilisersEconomic benefitsSeed treatment
The invention discloses an okra planting method. The okra planting method specifically comprises the steps of seed treating, seed coating germination accelerating, sowing and seedling raising, field managing and harvesting. According to the okra planting method, the disease resistance of okra is enhanced, growth is promoted, the yield of the okra is increased, the quality of the okra is improved, and the cultivated okra is rich in nutrition and tender and delicious in mouthfeel. The okra planting method is simple, easy to operate, low in cost, high in economic benefit and suitable for large-scale planting.
Owner:固镇县华原家庭农场
Seedling raising method of white gourd
InactiveCN106171353AMeet growthExtended growth cycleAlkali orthophosphate fertiliserExcrement fertilisersGourdDisease damage
The invention provides a seedling raising method of white gourd. The method comprises the following specific steps: preparing a seedling-raising substrate, accelerating germination of white gourd seeds, cultivating and raising seedlings. Through the seedling raising method of white gourd, the healthy growth of the seedlings is accelerated; the stress resistance of the seedlings is improved; the occurrence rate of pest and disease damage in the later period of the seedlings is reduced; the yield and the quality of the white gourd in the later period are ensured; the seedling raising method is simple and is easy to operate; the large-scale production is easy to implement; meanwhile, the planting cost is also reduced; the economic benefit is increased.
Owner:固镇县华丰农业有限公司
Method for organic celery seedling cultivation
InactiveCN106069095AChange in seed coat structureIncrease productionBiocidePlant growth regulatorsGrowth promotionWarm water
The invention discloses a method for organic celery seedling cultivation. The method comprises the steps of seed soaking and pregermination, bowl seedling culture, seedling culture management and transplantation. Compared with the prior art, the method has the advantages that by conducting warm water soaking, hot steam treatment, seed soaking in a seed-soaking reagent and microwave equipment treatment before pregermination, the seed coat structure of celery seeds can be effectively changed, water permeation is easier, quick germination is achieved, and survival rate is increased; composite soil covering is conducted after planting, and composite soil is light and breathable, so that effective sterilization and seedling growth promotion are achieved at the same time; through strict illumination and illumination intensity control, strong seedlings can be cultivated, and then the yield of celery after transplantation is increased.
Owner:固镇县华原家庭农场
Rapid propagation method for dysosma versipellis
ActiveCN105010146AFast growthPlant regeneration frequency is highPlant tissue cultureHorticulture methodsDysosma versipellisSomatic embryogenesis
The invention discloses a rapid propagation method for dysosma versipellis. The rapid propagation method includes the following steps that 1, sterile dysosma versipellis explants are manufactured; 2, the sterile explants are cultured through an induction medium to obtain embryonic callus; 3, the embryonic callus is cultured through a first multiplication medium, and the embryonic callus is subcultured for 2-3 generations to obtain subcultured embryonic callus; 4, the subcultured embryonic callus is cultured through a differentiation medium to obtain somatic embryos; 5, the somatic embryos are cultured through a second multiplication medium to obtain subcultured somatic embryos; 6, the subcultured somatic embryos are cultured through a plant regeneration medium to obtain dysosma versipellis plants. According to the rapid propagation method, the obtained dysosma versipellis embryogenic cell can induce regeneration of the dysosma versipellis plants, and the dysosma versipellis plants have the advantages of being high in growth rate and plant regeneration frequency, stable in character and the like.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
Disease-resistant and high-yield calendula officinalis cultivating method
The invention discloses a disease-resistant and high-yield calendula officinalis cultivating method. The method comprises the following steps: (1) selecting seeds: selecting a variety with high pigment, good disease resistance and high drying rate, removing impurities and dust, putting seeds in a dry, ventilation and shade place, and standing for over 10-12 hours for later use; (2) preparing a nutrient solution; and (3) performing seedling culture: carrying out seed sowing and seedling growing on a greenhouse seedbed in the last ten-day period of annual lunar March, and spraying 1000kg-1200kgof the nutrient solution prepared in the step (2) to each mu of the seedbed, wherein the width of the seedbed is 1.3m-1.4m, the depth of the seedbed is 25cm-35cm, and the temperature of the seedbed is20-30 DEG C. The calendula officinalis prepared by virtue of the disease-resistant and high-yield calendula officinalis cultivating method is high in yield, strong in stress resistance, low in disease and pest rates, environment-friendly and harmless and has relatively high use values and good application prospects.
Owner:HEFEI XUELIXIN AGRI TECH CO LTD
Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof
ActiveCN110117597AImprove salt toleranceHelp nurtureLigasesVector-based foreign material introductionGenetic engineeringMolecular breeding
The invention belongs to the field of genetic engineering, and particularly relates to a malus domestica 4-coumaric acid: coenzyme A ligase gene Md4CL4 and an encoding protein and application thereof.The gene is a 4CL gene Md4CL4 cloned from malus domestica. The gene is cloned from the malus domestica for the first time and provides genetic resources for plant molecular breeding. By utilizing a transgenic technology, an overexpression vector of the Md4CL4 gene is transferred into tobacco to obtain transgenic tobacco. The Md4CL4 gene is discovered from the malus domestica for the first time, due to high-response salt stress, a DNA fragment of a complete coding segment of the gene which is separated and cloned from the malus domestica and promotes salt stress tolerance is obtained, the functions of the gene are verified, and by utilizing the functions of the gene, it is finally discovered that the salt tolerance of a transgenic material obtained after the overexpression of the Md4CL4 gene in the tobacco is significantly improved. By means of the gene, the cultivation of new plant varieties with high salt tolerance is facilitated.
Owner:SHANDONG INST OF POMOLOGY
Molecular marker for wheat functional genes NAM-B1 and application of molecular marker
InactiveCN107012142AEasy to digQuick digMicrobiological testing/measurementDNA/RNA fragmentationMolecular breedingPolymerase chain reaction
The invention relates to a molecular marker for wheat functional genes NAM-B1 and application of the molecular marker. Primer pair sequences for carrying out specific PCR (polymerase chain reaction) amplification on the molecular marker SpNAM-B1 for the wheat functional genes NAM-B1 are shown as SpNAM-B1-F and SpNAM-B1-R. The molecular marker and the application have the advantages that whether wheat contains the functional genes NAM-B1 or not can be identified by the aid of the molecular marker; the molecular marker can be used for carrying out high-protein molecular breeding on the wheat, the functional genes NAM-B1 for controlling grain protein contents can be transferred into receptor materials without the functional genes under assisted selection effects of the molecular markers, and accordingly the molecular marker and the application are beneficial to cultivating high-protein new wheat varieties with high iron and zinc contents.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Cotton casein kinase gene and its application
InactiveCN102925466AReduce workloadBiological function elucidationTransferasesGenetic engineeringTemperature stressAgricultural science
The invention discloses a cotton casein kinase gene and its application, and relates to a separation cloning, a function verification and an application of a gene GhCKI used for controlling anther growth and identified in a cotton anther expression spectrum. There is a 72% homology between the protein coded by the GhCKI gene and the protein coded by an Arabidopis thaliana ATCKL2 gene, and the GhCKI gene preferentially expresses in the anther, has an anther growth controlling function, can be applied to the sterile line cultivation of a plurality of plants through genetic transformation, and simultaneously can be used as a marker gene of the plant anther fertility stability under high temperature stress.
Owner:HUAZHONG AGRI UNIV
Method of using selenium-enriched coating to plant selenium-enriched pea
InactiveCN106804156AImprove disease resistanceStrong disease resistanceSuperphosphatesMagnesium fertilisersPurplish redSeedling
The invention relates to a method of using a selenium-enriched coating to plant selenium-enriched pea. The method is characterized by including following steps: a, preparing soaking liquid; b, preparing a sodium selenite coating agent; c, soaking seeds; d, coating; e, seeding and growing seedlings; f, performing field management; g, harvesting. Potassium permanganate is added into the soaking liquid which is purple after soaking, so that pea seeds soaked by the sodium selenite can be prevented from being eaten mistakenly, and effects of sterilizing and disinfecting and promoting pea yield increase. The method is simple to operate, easy to implement, low in cost, good in effect, capable of remarkably improving quality and taste of pea and convenient for large-scale production, and pea produced by the method is uniform in selenium content.
Owner:ENSHI PREFECTURE DIYIJIA INTPROP SERVICES CO LTD
Seed germination treatment method of Chinese rhus succedanea
The invention relates to a method for treating plant seeds, specifically a method for treating the germination of Chinese wild sumac seeds. The seeds are collected together with the peel, the seeds are exposed to the sun and separated from the peel, dried in the shade and stored until the spring of the second year, and soaked in carbendazim solution: then use Soak in warm water, wash the seeds with clean water, drain the seeds and sow. The Chinese wild sumac seed germination treatment method provided by the present invention uses physical stimulation as a means to effectively break the characteristics of hard, dense and waxy seeds of wild sumac seeds, which are not easy to germinate, and contribute to the cultivation of high-quality seedlings of wild sumac and germination The efficiency reaches more than 90%. Compared with the conventional seed treatment method, the seed treatment time is shortened by two-thirds, and the seeds can be processed in batches on a large scale. The cost is low, the method is simple, and it is easy to operate.
Owner:SICHUAN ACAD OF FORESTRY
Automatic soilless culture production line
ActiveCN113940268AHelps fixHelps fix soilless cultivationHarvestersAgriculture gas emission reductionAgricultural scienceElectric machine
The invention discloses an automatic soilless culture production line, and belongs to the technical field of soilless culture. The automatic soilless culture production line is characterized by comprising a conveyor belt group, supporting frames and a plurality of soilless culture devices, wherein, each soilless culture device comprises a plurality of culture grooves; vegetable fixing discs are arranged on the inner walls of the culture grooves; two sliding grooves are formed in the inner wall of each culture groove, first stepping motors are arranged in the sliding grooves, and the first stepping motors are fixedly connected with the vegetable fixing discs; supporting transverse plates are fixedly connected to the outer sides of the two supporting frames correspondingly, and strip-shaped grooves are formed in the two supporting transverse plates correspondingly; second stepping motors and third stepping motors are installed in the strip-shaped grooves, the second stepping motors and the third stepping motors are connected with electric telescopic rods, rotating motors are arranged at the ends of the electric telescopic rods, and the rotating motors are connected with rotating parts; and a transverse part is fixedly connected between every two rotating parts, a plurality of sliding motors are arranged in the transverse parts, and the sliding motors are connected with picking blades. The automatic soilless culture production line is mainly used for automatically planting and picking vegetables, and the working efficiency and the automation degree are effectively improved.
Owner:SOUTH CHINA AGRI UNIV
Method for planting selenium-rich soybeans by using selenium-rich coatings
InactiveCN106613283AImprove disease resistanceStrong disease resistanceSuperphosphatesMagnesium fertilisersPurplish redSeedling
The invention relates to a method for planting selenium-rich soybeans by using selenium-rich coatings. The method is characterized by including the following steps: 1, preparing a soaking solution; 2, preparing a sodium selenite coating agent; 3, soaking seeds; 4, conducting coating; 5, conducting sowing and culturing seedlings; 6, conducting field management; 7, harvesting the selenium-rich soybeans. Potassium permanganate is added into the soaking solution, the solution appears purple after soaking, therefore, the soybean seeds soaked with sodium selenite are prevented from being eaten by people by mistake, disinfection and sterilization are achieved, and the yield of the soybeans is promoted. The method is simple and easy to implement, low in cost, good in effect and capable of remarkably improving the quality and taste of the soybeans, the selenium content of the produced soybeans is consistent, and scale production is convenient to achieve.
Owner:ENSHI PREFECTURE DIYIJIA INTPROP SERVICES CO LTD
Application of osnpf5.11 gene in improving rice yield
ActiveCN107056909BIncrease the number of tillersIncrease productionPlant peptidesFermentationBiotechnologyGrowth plant
The invention discloses application of an OsNPF5.11 gene to improvement in rice yield and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF5.11 gene encoding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Through constructing a rice OsNPF5.11 gene overexpressing plant and an OsNPF5.11 gene interference plant, the increase in the normal number of tillers of rice and the number of grouting grains per plant through improving the expression of the OsNPF5.11 gene can be discovered; therefore, the OsNPF5.11 gene can be used in rice breeding to improve the rice yield. The OsNPF5.11 gene has an important application value in aspects of elaborating the influence of nitrogen elements on plant growth and development process and improving the rice plant type.
Owner:WUHAN INST OF BIOENG
Gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of gene GauRev2
ActiveCN113234729ASolve production problemsHelp nurturePlant peptidesFermentationBiotechnologyHeterologous
The invention discloses a gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of the gene GauRev2. The gene has a nucleotide sequence as shown in SEQ ID NO. 1. According to the invention, the expression of the GausREV2 gene in cotton is inhibited through a virus-mediated gene silencing technology, and after a verticillium wilt spore suspension is inoculated, the silencing plant is more susceptible to diseases. In arabidopsis thaliana, resistance to verticillium wilt can be remarkably improved through heterologous overexpression of the GausREV2. Discovery and utilization of the GausREV2 are beneficial to cultivation of cotton varieties resistant to verticillium wilt, and the problems in cotton production are solved. The molecular mechanism of the GausREV2 on verticillium wilt resistance is deeply researched, so that the GausREV2 has important theoretical significance for breeding cotton varieties with high verticillium wilt resistance by cotton breeders.
Owner:NANJING AGRICULTURAL UNIVERSITY
Application of nitrogen transport gene OsNPF7.4 to rice selecting breeding
ActiveCN106947777AEnhanced tillering abilityIncrease the number of tillersPlant peptidesFermentationAgricultural sciencePlant genetic engineering
The invention discloses application of nitrogen transport gene OsNPF7.4 to rice selecting breeding and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF7.4 gene coding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Normal rice tiller number, spike number and grouting grain number can be increased by reducing OsNPF7.4 gene expression after a rice OsNPF7.4 gene interfering plant and an OsNPF7.4 gene over-expression plant are built, therefore, the OsNPF7.4 gene can be applied to rice selecting breeding to improve rice yield. The OsNPF7.4 gene has important application value in the aspect of elaborating the influence of nitrogen transport to plant growing and developing processes.
Owner:WUHAN INST OF BIOENG
Pre-harvest management method for slowing down softening of muskmelon fruits
InactiveCN107409804AImprove disease resistanceStrong disease resistanceSeed and root treatmentHorticulture methodsDiseasePlant disease
The invention belongs to the technical field of muskmelon management and particularly relates to a pre-harvest management method for slowing down softening of muskmelon fruits. The method comprises the following steps: treatment of muskmelon seeds, management in two weeks after flowering, management of a reticulation forming period and pre-harvest 48-hour management. Compared with the prior art, the method has the advantages that the root activity of seedlings can be improved, and the stem diameter of a plant can be effectively increased, so that the cultivation of strong seedlings is facilitated; the content of hydrogen peroxide of the plant can be favorably increased till a young fruit period of muskmelons after flowering, so that the disease resistance of the plant is improved, and fungus infection to the plant in the period can be avoided; in the reticulation forming period, the deposition of lignin of the plant can be promoted, so that the permeation and extension of diseases can be prevented; the whole plant is sprayed with jasmonic acid methyl ester solution in 48h before harvest, so that the muskmelon fruits have a certain firmness, and the fruit firmness and the content of protopectin when the muskmelons are harvested can be effectively improved, therefore, the purpose of slowing down the softening of the muskmelon fruits is achieved.
Owner:界首市海棠湾生态农业科技有限公司
A kind of planting method of organic vegetable okra
InactiveCN103931377BImprove securityEnsure safetyPlant protectionSeed coating/dressingNatural sourceSeed treatment
Owner:GUIGANG HEAN HUIXING AGRI SCI & TECH
Device and method for rapidly separating monospora strains of rice blast germs
PendingCN111304061ASolve the cumbersomeSolve the costBioreactor/fermenter combinationsFungiEngineeringBiology
The invention provides a device for rapidly separating monospora strains of rice blast germs. The device comprises an operation platform, a driving tank, a teasing needle and a strain bearing platform, wherein a light hole is formed in the operation platform, a driving system is arranged in the driving tank, the strain bearing platform is arranged along the circumferential direction of the light hole and is used for bearing a to-be-operated rice straw, the teasing needle is driven by the driving system to move along the horizontal and vertical directions, the tip of the teasing needle is in contact with the rice straw.
Owner:XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
Application of Rice osrhd1-1 Gene in the Breeding of Rice Male Male Sterile Line
The invention discloses the application of the rice OsRHD1‑1 gene in the cultivation of rice male sterility lines. The present invention constructs a gene knockout vector by designing a knockout target of the OsRHD1-1 gene, and performs genetic transformation in rice, and finds that the loss of OsRHD1-1 gene function can lead to pollen sterility, which is helpful for a new type of male sterile line The development of hybrid breeding is facilitated.
Owner:ZHEJIANG UNIV
A kind of artificial cultivation method of dominant symbiotic mycorrhizal fungus of Chestnut
ActiveCN105087400BHigh infection rateLarge diameterFungiMicroorganism based processesHyphaHyphomycetes
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
A kind of processing method of lacquer tree seedling cultivation seed
The invention provides a processing method of sumac seedling seeds. The method comprises the steps of storing sumac seeds in sand, soaking the seeds in saline solution, and cleaning; soaking the seeds in washing powder water, rubbing the surfaces of the seeds to remove wax, and putting the seeds into a woven bag; spraying warm water onto the seeds every other 2 hours until the seeds are expanded and the seed coats are softened; putting the seeds into a breathable bamboo frame or a plastic frame, and watering until the seeds crack and sprout. According to the processing method, the characteristics that the sumac seedling seeds have after-ripening embryo, and seed shells are firm, seed coats are compact and the waxiness is thick can be effectively broken, the cultivation of the high-quality sumac nursery stock can be facilitated, the germination rate of the sumac seeds reaches more than 50% after 5-6 days, the sumac seeds can be planted in the nursery garden land by broadcast sowing or hill-seeding in the seventh day, the germination rate reaches up to more than 95% within 15 days after sowing, and 60% of seeds can germinate within a week. According to the processing method, the germination period is short, more than half time can be shortened, the seeds can be processed in batches on a large scale, and the cost is low; furthermore, a chemical reagent with high harmfulness is eliminated, and the processing method has the characteristics of being safe, environmentally friendly and easy to operate.
Owner:云南盛禾生态农业科技发展有限公司
Vegetable high-temperature stress regulation and control mixture and application method thereof
InactiveCN110981593AHigh activityEnhance active oxygen scavenging abilityBiocidePlant growth regulatorsPhosphateAlcohol sugars
The invention provides a vegetable high-temperature stress regulation and control mixture which comprises the following raw material components in percentage by weight: 0.05-0.15% of salicylic acid, 0.02%-0.05% of brassinolide; 0.3%-0.5% of urea; 0.05%-0.1% of gamma-aminobutyric acid; 0.3%-0.5% of glucose; 0.02% to 0.08% of mepiquat chloride; 0.1%-0.5% of potassium dihydrogen phosphate; 0.07%-0.1%of sugar alcohol boron; 0.07%-0.1% of sugar alcohol iron; 0.08%-0.1% of sugar alcohol calcium; 0.1%-0.3% of zinc sulfate heptahydrate (ZnSO<4>*7H<2>O); 0.1% of an agricultural organic silicon spreader; and 97.42%-98.74% of water. When the vegetable high-temperature stress regulation and control mixture prepared by the invention is adopted, the protective enzyme activity can be obviously improved;the content of malondialdehyde (MDA) is reduced to 1.62-1.67 [mu] mol.g <-1 > FW, in addition, the photosynthetic performance of leaves can be improved, the diseases such as element absorption obstacle, high-temperature nutrient deficiency of plants, root aging of vegetables and root activity reduction can be prevented and treated, the malformed fruit rate is reduced, and the effects of increasing the yield and improving the quality of over-summer vegetables are achieved.
Owner:WEIFANG UNIV OF SCI & TECH
Method for planting selenium-rich broad beans with selenium-rich coatings
InactiveCN106665099AImprove disease resistanceStrong disease resistanceSuperphosphatesMagnesium fertilisersBiotechnologyPurplish red
The invention relates to a method for planting selenium-rich broad beans with selenium-rich coatings. The method includes the following steps: 1, soaking solution preparation; 2, sodium selenite coating agent preparation; 3, seed soaking; 4, coating; 5, sowing and seedling culturing; 6, field management; 7, harvesting. Potassium permanganate is added in a soaking solution, after being soaked, broad bean seeds become purplish red, the broad bean seeds soaked with sodium selenite are prevented from being eaten by people by mistakes, disinfection and sterilization are achieved, and the yield of the broad beans is promoted. The method is simple and easy to operate, low in cost, good in effect, capable of significantly improving the quality and taste of the broad beans, and the obtained broad beans are same in selenium content, and large-scale production is conveniently achieved.
Owner:ENSHI PREFECTURE DIYIJIA INTPROP SERVICES CO LTD
BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene
ActiveCN114231542AHelp nurtureExcellent resistance geneMicrobiological testing/measurementPlant peptidesBhlh genesMolecular biology
The invention discloses a bHLH gene influencing the salt tolerance of populus sutchuenensis and application thereof.The sequence of the bHLH gene is shown in a sequence table 1, the amino acid sequence of the bHLH gene is shown in a sequence table 2, and the application of the bHLH gene is that knockout expression of the bHLH gene influences the salt tolerance of populus sutchuenensis, so that breeding of salt-tolerant poplar new germplasm is facilitated; research results provide excellent resistance genes for improvement of salt-tolerant varieties of poplars, and have important scientific significance.
Owner:SHENYANG AGRI UNIV
Application of amino acid transport gene osaap3 in rice breeding
ActiveCN106518993BEnhanced tillering abilityIncrease the number of tillersHydrolasesFermentationTransfer genePlant genetic engineering
The invention discloses application of an amino acid transporter gene OsAAP3 in rice seed selection, and belongs to the technical field of genetic engineering. An amino acid sequence of encoded protein of the gene OsAAP3 is specified in SEQ ID NO.1, and a Cdna sequence is specified in SEQ ID NO.2. According to the application of the amino acid transporter gene OsAAP3 in rice seed selection, by constructing a rice plant which is interfered by the gene OsAAP3, and constructing a rice plant which is over-expressed by the gene OsAAP3, it is discovered that reduction of genetic expression of the gene OsAAP3 can increase tiller number of the rice and spike number in each rice plant, and therefore the gene OsAAP3 can be used in rice seed selection for increasing the rice yield. The gene OsAAP3 has important application value in expounding influence of amino acid transportation on growth and development process of the plant.
Owner:WUHAN INST OF BIOENG
Substrate for raising seedlings from seeds of Rhizoma Areactylodis Lanceae and preparation method and application of substrate
The invention discloses a substrate for raising seedlings from seeds of Rhizoma Areactylodis Lanceae and a preparation method and application of the substrate. The substrate includes peat, vermiculite, perlite and rotten chicken manure at a preferable ratio of 3:1:1, and the amount of the rotten chicken manure is 5-8 kg / m<3>. Strong seedlings can be raised when the substrate is used for raising seedlings from seeds of Rhizoma Areactylodis Lanceae, and a fertilization and management method which is matched with the substrate is simple, and has easy implementation; after optimization is carriedout, and the method which meets the suitable formula ratio of the seedling substrate of Rhizoma Areactylodis Lanceae and the fertilization ratio and management in the seedling period is proposed through comprehensive evaluation of seedling fresh weight, the chlorophyll content, root vitality and other indicators; and the produced seedlings are robust, the quality is uniform, the weight of the seedlings is 2 times the weight of even-aged Rhizoma Areactylodis Lanceae seedlings which are obtained through open culture, and the method is of great significance to cultivation of high-quality RhizomaAreactylodis Lanceae.
Owner:甘肃广药白云山中药科技有限公司 +2
Application of Nitrogen Transport Gene osnpf8.1 in Improving Rice Tiller Number
ActiveCN107012153BEnhanced tillering abilityIncrease productionPlant peptidesFermentationBiotechnologyGrowth plant
The invention discloses application of a nitrogen nutrition transport gene OsNPF8.1 in improvement of rice tiller number and belongs to the field of plant genetic engineering. An OsNPF8.1 gene encoded protein has an amino acid sequence shown as SEQ ID NO.1, and a cDNA sequence of the protein is shown as SEQ ID NO.2. By establishing rice OsNPF8.1 gene overexpression plants and OsNPF8.1 gene interference plants, the tiller number of normal rice and the spike number of each rice plant can be increased by improving the OsNPF8.1 gene expression. Therefore, the gene OsNPF8.1 can be used in rice breeding for improving the rice yield. The gene OsNPF8.1 has important application values in the aspect of explaining influences of nitrogen on plant growth and development and the aspect of improving the rice plant type.
Owner:WUHAN INST OF BIOENG
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