39results about How to "Help nurture" patented technology

The invention discloses application of a nitrate radical transporter gene OsNRT1.8 in rice breeding and belongs to the field of plant genetic engineering. The protein encoding amino acid sequence of the gene OsNRT1.8 is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. By establishing rice gene OsNRT1.8 overexpression plant and a gene OsNRT1.8 interference plant, it is found that normal rice tiller number and spike number of each plant can be increased by improving gene OsNRT1.8 expression. Therefore, the gene OsNRT1.8 can be used for increasing rice yield in rice breeding. The gene OsNRT1.8 has an important application value on the aspects of elaboration about nitrogen influence on plant growth and development and improvement of rice plant type.

The invention discloses an okra planting method. The okra planting method specifically comprises the steps of seed treating, seed coating germination accelerating, sowing and seedling raising, field managing and harvesting. According to the okra planting method, the disease resistance of okra is enhanced, growth is promoted, the yield of the okra is increased, the quality of the okra is improved, and the cultivated okra is rich in nutrition and tender and delicious in mouthfeel. The okra planting method is simple, easy to operate, low in cost, high in economic benefit and suitable for large-scale planting.

The invention discloses a method for organic celery seedling cultivation. The method comprises the steps of seed soaking and pregermination, bowl seedling culture, seedling culture management and transplantation. Compared with the prior art, the method has the advantages that by conducting warm water soaking, hot steam treatment, seed soaking in a seed-soaking reagent and microwave equipment treatment before pregermination, the seed coat structure of celery seeds can be effectively changed, water permeation is easier, quick germination is achieved, and survival rate is increased; composite soil covering is conducted after planting, and composite soil is light and breathable, so that effective sterilization and seedling growth promotion are achieved at the same time; through strict illumination and illumination intensity control, strong seedlings can be cultivated, and then the yield of celery after transplantation is increased.

The invention discloses a rapid propagation method for dysosma versipellis. The rapid propagation method includes the following steps that 1, sterile dysosma versipellis explants are manufactured; 2, the sterile explants are cultured through an induction medium to obtain embryonic callus; 3, the embryonic callus is cultured through a first multiplication medium, and the embryonic callus is subcultured for 2-3 generations to obtain subcultured embryonic callus; 4, the subcultured embryonic callus is cultured through a differentiation medium to obtain somatic embryos; 5, the somatic embryos are cultured through a second multiplication medium to obtain subcultured somatic embryos; 6, the subcultured somatic embryos are cultured through a plant regeneration medium to obtain dysosma versipellis plants. According to the rapid propagation method, the obtained dysosma versipellis embryogenic cell can induce regeneration of the dysosma versipellis plants, and the dysosma versipellis plants have the advantages of being high in growth rate and plant regeneration frequency, stable in character and the like.

The invention discloses a disease-resistant and high-yield calendula officinalis cultivating method. The method comprises the following steps: (1) selecting seeds: selecting a variety with high pigment, good disease resistance and high drying rate, removing impurities and dust, putting seeds in a dry, ventilation and shade place, and standing for over 10-12 hours for later use; (2) preparing a nutrient solution; and (3) performing seedling culture: carrying out seed sowing and seedling growing on a greenhouse seedbed in the last ten-day period of annual lunar March, and spraying 1000kg-1200kgof the nutrient solution prepared in the step (2) to each mu of the seedbed, wherein the width of the seedbed is 1.3m-1.4m, the depth of the seedbed is 25cm-35cm, and the temperature of the seedbed is20-30 DEG C. The calendula officinalis prepared by virtue of the disease-resistant and high-yield calendula officinalis cultivating method is high in yield, strong in stress resistance, low in disease and pest rates, environment-friendly and harmless and has relatively high use values and good application prospects.

The invention belongs to the field of genetic engineering, and particularly relates to a malus domestica 4-coumaric acid: coenzyme A ligase gene Md4CL4 and an encoding protein and application thereof.The gene is a 4CL gene Md4CL4 cloned from malus domestica. The gene is cloned from the malus domestica for the first time and provides genetic resources for plant molecular breeding. By utilizing a transgenic technology, an overexpression vector of the Md4CL4 gene is transferred into tobacco to obtain transgenic tobacco. The Md4CL4 gene is discovered from the malus domestica for the first time, due to high-response salt stress, a DNA fragment of a complete coding segment of the gene which is separated and cloned from the malus domestica and promotes salt stress tolerance is obtained, the functions of the gene are verified, and by utilizing the functions of the gene, it is finally discovered that the salt tolerance of a transgenic material obtained after the overexpression of the Md4CL4 gene in the tobacco is significantly improved. By means of the gene, the cultivation of new plant varieties with high salt tolerance is facilitated.

The invention relates to a molecular marker for wheat functional genes NAM-B1 and application of the molecular marker. Primer pair sequences for carrying out specific PCR (polymerase chain reaction) amplification on the molecular marker SpNAM-B1 for the wheat functional genes NAM-B1 are shown as SpNAM-B1-F and SpNAM-B1-R. The molecular marker and the application have the advantages that whether wheat contains the functional genes NAM-B1 or not can be identified by the aid of the molecular marker; the molecular marker can be used for carrying out high-protein molecular breeding on the wheat, the functional genes NAM-B1 for controlling grain protein contents can be transferred into receptor materials without the functional genes under assisted selection effects of the molecular markers, and accordingly the molecular marker and the application are beneficial to cultivating high-protein new wheat varieties with high iron and zinc contents.

The invention discloses a cotton casein kinase gene and its application, and relates to a separation cloning, a function verification and an application of a gene GhCKI used for controlling anther growth and identified in a cotton anther expression spectrum. There is a 72% homology between the protein coded by the GhCKI gene and the protein coded by an Arabidopis thaliana ATCKL2 gene, and the GhCKI gene preferentially expresses in the anther, has an anther growth controlling function, can be applied to the sterile line cultivation of a plurality of plants through genetic transformation, and simultaneously can be used as a marker gene of the plant anther fertility stability under high temperature stress.

The invention relates to a method of using a selenium-enriched coating to plant selenium-enriched pea. The method is characterized by including following steps: a, preparing soaking liquid; b, preparing a sodium selenite coating agent; c, soaking seeds; d, coating; e, seeding and growing seedlings; f, performing field management; g, harvesting. Potassium permanganate is added into the soaking liquid which is purple after soaking, so that pea seeds soaked by the sodium selenite can be prevented from being eaten mistakenly, and effects of sterilizing and disinfecting and promoting pea yield increase. The method is simple to operate, easy to implement, low in cost, good in effect, capable of remarkably improving quality and taste of pea and convenient for large-scale production, and pea produced by the method is uniform in selenium content.

The invention relates to a method for treating plant seeds, specifically a method for treating the germination of Chinese wild sumac seeds. The seeds are collected together with the peel, the seeds are exposed to the sun and separated from the peel, dried in the shade and stored until the spring of the second year, and soaked in carbendazim solution: then use Soak in warm water, wash the seeds with clean water, drain the seeds and sow. The Chinese wild sumac seed germination treatment method provided by the present invention uses physical stimulation as a means to effectively break the characteristics of hard, dense and waxy seeds of wild sumac seeds, which are not easy to germinate, and contribute to the cultivation of high-quality seedlings of wild sumac and germination The efficiency reaches more than 90%. Compared with the conventional seed treatment method, the seed treatment time is shortened by two-thirds, and the seeds can be processed in batches on a large scale. The cost is low, the method is simple, and it is easy to operate.

The invention discloses an automatic soilless culture production line, and belongs to the technical field of soilless culture. The automatic soilless culture production line is characterized by comprising a conveyor belt group, supporting frames and a plurality of soilless culture devices, wherein, each soilless culture device comprises a plurality of culture grooves; vegetable fixing discs are arranged on the inner walls of the culture grooves; two sliding grooves are formed in the inner wall of each culture groove, first stepping motors are arranged in the sliding grooves, and the first stepping motors are fixedly connected with the vegetable fixing discs; supporting transverse plates are fixedly connected to the outer sides of the two supporting frames correspondingly, and strip-shaped grooves are formed in the two supporting transverse plates correspondingly; second stepping motors and third stepping motors are installed in the strip-shaped grooves, the second stepping motors and the third stepping motors are connected with electric telescopic rods, rotating motors are arranged at the ends of the electric telescopic rods, and the rotating motors are connected with rotating parts; and a transverse part is fixedly connected between every two rotating parts, a plurality of sliding motors are arranged in the transverse parts, and the sliding motors are connected with picking blades. The automatic soilless culture production line is mainly used for automatically planting and picking vegetables, and the working efficiency and the automation degree are effectively improved.

The invention relates to a method for planting selenium-rich soybeans by using selenium-rich coatings. The method is characterized by including the following steps: 1, preparing a soaking solution; 2, preparing a sodium selenite coating agent; 3, soaking seeds; 4, conducting coating; 5, conducting sowing and culturing seedlings; 6, conducting field management; 7, harvesting the selenium-rich soybeans. Potassium permanganate is added into the soaking solution, the solution appears purple after soaking, therefore, the soybean seeds soaked with sodium selenite are prevented from being eaten by people by mistake, disinfection and sterilization are achieved, and the yield of the soybeans is promoted. The method is simple and easy to implement, low in cost, good in effect and capable of remarkably improving the quality and taste of the soybeans, the selenium content of the produced soybeans is consistent, and scale production is convenient to achieve.

The invention discloses a gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of the gene GauRev2. The gene has a nucleotide sequence as shown in SEQ ID NO. 1. According to the invention, the expression of the GausREV2 gene in cotton is inhibited through a virus-mediated gene silencing technology, and after a verticillium wilt spore suspension is inoculated, the silencing plant is more susceptible to diseases. In arabidopsis thaliana, resistance to verticillium wilt can be remarkably improved through heterologous overexpression of the GausREV2. Discovery and utilization of the GausREV2 are beneficial to cultivation of cotton varieties resistant to verticillium wilt, and the problems in cotton production are solved. The molecular mechanism of the GausREV2 on verticillium wilt resistance is deeply researched, so that the GausREV2 has important theoretical significance for breeding cotton varieties with high verticillium wilt resistance by cotton breeders.

The invention discloses application of nitrogen transport gene OsNPF7.4 to rice selecting breeding and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF7.4 gene coding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Normal rice tiller number, spike number and grouting grain number can be increased by reducing OsNPF7.4 gene expression after a rice OsNPF7.4 gene interfering plant and an OsNPF7.4 gene over-expression plant are built, therefore, the OsNPF7.4 gene can be applied to rice selecting breeding to improve rice yield. The OsNPF7.4 gene has important application value in the aspect of elaborating the influence of nitrogen transport to plant growing and developing processes.

The invention provides a device for rapidly separating monospora strains of rice blast germs. The device comprises an operation platform, a driving tank, a teasing needle and a strain bearing platform, wherein a light hole is formed in the operation platform, a driving system is arranged in the driving tank, the strain bearing platform is arranged along the circumferential direction of the light hole and is used for bearing a to-be-operated rice straw, the teasing needle is driven by the driving system to move along the horizontal and vertical directions, the tip of the teasing needle is in contact with the rice straw.

The invention relates to a method for planting selenium-rich broad beans with selenium-rich coatings. The method includes the following steps: 1, soaking solution preparation; 2, sodium selenite coating agent preparation; 3, seed soaking; 4, coating; 5, sowing and seedling culturing; 6, field management; 7, harvesting. Potassium permanganate is added in a soaking solution, after being soaked, broad bean seeds become purplish red, the broad bean seeds soaked with sodium selenite are prevented from being eaten by people by mistakes, disinfection and sterilization are achieved, and the yield of the broad beans is promoted. The method is simple and easy to operate, low in cost, good in effect, capable of significantly improving the quality and taste of the broad beans, and the obtained broad beans are same in selenium content, and large-scale production is conveniently achieved.

The invention discloses a bHLH gene influencing the salt tolerance of populus sutchuenensis and application thereof.The sequence of the bHLH gene is shown in a sequence table 1, the amino acid sequence of the bHLH gene is shown in a sequence table 2, and the application of the bHLH gene is that knockout expression of the bHLH gene influences the salt tolerance of populus sutchuenensis, so that breeding of salt-tolerant poplar new germplasm is facilitated; research results provide excellent resistance genes for improvement of salt-tolerant varieties of poplars, and have important scientific significance.

The invention discloses a substrate for raising seedlings from seeds of Rhizoma Areactylodis Lanceae and a preparation method and application of the substrate. The substrate includes peat, vermiculite, perlite and rotten chicken manure at a preferable ratio of 3:1:1, and the amount of the rotten chicken manure is 5-8 kg/m<3>. Strong seedlings can be raised when the substrate is used for raising seedlings from seeds of Rhizoma Areactylodis Lanceae, and a fertilization and management method which is matched with the substrate is simple, and has easy implementation; after optimization is carriedout, and the method which meets the suitable formula ratio of the seedling substrate of Rhizoma Areactylodis Lanceae and the fertilization ratio and management in the seedling period is proposed through comprehensive evaluation of seedling fresh weight, the chlorophyll content, root vitality and other indicators; and the produced seedlings are robust, the quality is uniform, the weight of the seedlings is 2 times the weight of even-aged Rhizoma Areactylodis Lanceae seedlings which are obtained through open culture, and the method is of great significance to cultivation of high-quality RhizomaAreactylodis Lanceae.