A kind of artificial cultivation method of dominant symbiotic mycorrhizal fungus of Chestnut

A symbiotic mycorrhizal and artificial culture technology, applied in microorganism-based methods, biochemical equipment and methods, horticultural methods, etc., can solve the problems of difficulty in the isolation and cultivation of mycorrhizal fungi, complex symbiotic relationships, slow growth and development, etc. The effect of large diameter, improving physiological activity and high mycorrhizal infection rate
CN105087400BActive Publication Date: 2019-03-15CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Publication Date
2019-03-15

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Abstract

The invention relates to an artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. The method comprises the following steps: (1) taking a fruiting body of mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils., and collecting fungus context issue blocks at the intersecting part of a fungus cap and a fungus stipe; (2) putting the fungus context tissue blocks in a culture plate, culturing the fungus context tissue blocks at the temperature of 25-28 DEG C until mycelia are germinated by the fungus context issue blocks, transferring the mycelia, and then cultivating the mycelia at the temperature of 25-28 DEG C until the mycelia grow to cover the culture plate to obtain purified mycelia; (3) selecting the purified mycelia, and inoculating the selected purified mycelia to an improved MMN liquid culture medium for fermental cultivation, thus obtaining a bacterium solution; (4) inoculating the bacterium solution to an enlarged cultivating material, and cultivating the purified mycelia at the temperature of 25-28 DEG C until the purified mycelia grow all over a cultivating container to obtain mycorrhizal fungi inoculant of Castanea henryi (Skam) Rehd. et Wils. The artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. is simple in operation; when mycorrhizal fungi cultivated and obtained through the artificial cultivating method is inoculated to seedlings of Castanea henryi Rehd. et Wils., the impregnating rate is high.
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Description

technical field

[0001] The invention belongs to the field of artificial cultivation of mycorrhizal fungi, and in particular relates to a method for artificial cultivation of mycorrhizal fungi. Background technique

[0002] Ectomycorrhizal fungi (ECMF) is formed by the symbiosis of plant vegetative roots and soil fungi, and is ubiquitous in nature. Ectomycorrhizal fungi can expand the area of ​​host plant roots to absorb nutrients, produce a variety of organic acids, enzymes, antibiotics and other substances, and accelerate the cycle of organic matter and inorganic matter such as nitrogen, phosphorus, potassium, calcium, and magnesium in the ecosystem. Improve the nutritional status of planting soil, promote the growth of host plants, and at the same time have an antagonistic effect on root soil-borne diseases, and can enhance the stress resistance of plants. Ectomycorrhizal fungi affect the composition and quantity of microorganisms in the rhizosphere of host plants. The ap...

Claims

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