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Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof

A technology for coding protein and coumaric acid, which is applied in the field of genetic engineering, can solve problems such as the application of 4CL coding genes, and achieve the effect of improving salt tolerance and improving salt tolerance

Active Publication Date: 2019-08-13
SHANDONG INST OF POMOLOGY
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  • Abstract
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Problems solved by technology

In the prior art, there is no application of 4CL coding gene in the regulation of salt tolerance

Method used

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  • Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof
  • Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof
  • Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof

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Embodiment Construction

[0039] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0040] The invention provides an apple 4-coumaric acid coenzyme A ligase ( Md4CL4 ) gene and its encoded protein and application, specific examples are as follows:

[0041] An apple 4-coumaric acid coenzyme A ligase ( Md4CL4 ) gene and its encoded protein and application, including the following steps:

[0042] (1) Apple 4CL Gene identification: using the Apple Reference Genome v1.0 sequence (downloaded from the GDR database, http: / / www.rosaceae.org / ). Download the HMM (Hidden Markov Model) files PF00501 (AMP-binding domain) and PF13193 (AMP-binding domain_C domain) from the Pfam database (http: / / pfam.xfam.org; version 31.0), and use the HMMER software ( Version 3.1b2) of the hmmsearch function searches the apple genome (default E value 0.05). Arabidopsis ( Arabidopsis...

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Abstract

The invention belongs to the field of genetic engineering, and particularly relates to a malus domestica 4-coumaric acid: coenzyme A ligase gene Md4CL4 and an encoding protein and application thereof.The gene is a 4CL gene Md4CL4 cloned from malus domestica. The gene is cloned from the malus domestica for the first time and provides genetic resources for plant molecular breeding. By utilizing a transgenic technology, an overexpression vector of the Md4CL4 gene is transferred into tobacco to obtain transgenic tobacco. The Md4CL4 gene is discovered from the malus domestica for the first time, due to high-response salt stress, a DNA fragment of a complete coding segment of the gene which is separated and cloned from the malus domestica and promotes salt stress tolerance is obtained, the functions of the gene are verified, and by utilizing the functions of the gene, it is finally discovered that the salt tolerance of a transgenic material obtained after the overexpression of the Md4CL4 gene in the tobacco is significantly improved. By means of the gene, the cultivation of new plant varieties with high salt tolerance is facilitated.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to an apple 4-coumaric acid:coenzyme A ligase gene Md4CL4 And its encoded protein and application. Background technique [0002] Severe drought caused by global climate change and unreasonable human cultivation have caused the problem of secondary salinization of soil to become increasingly prominent. Salinized soil accounts for 7.6% of the world's land area, while my country's salinized soil area is 27 million hectares, accounting for the total 28.1% of the cultivated land area. Soil salinization has become an important limiting factor restricting agricultural development. apple( Malus domestica ) is one of the most economically important horticultural crops in the world and one of the most widely cultivated fruit tree species. Due to the genus Malus ( Malus ) has different phytogeographical origins, resulting in rich variation in variety types. Currently, about 14 types ...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12N9/00C12N15/82A01H5/00A01H6/82
CPCC12N9/93C12Y602/01009C12N15/8205C12N15/8273
Inventor 王海波何平常源升王森王传增何晓文徐丽李林光
Owner SHANDONG INST OF POMOLOGY
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