Application of osnpf5.11 gene in improving rice yield

A gene and rice technology, applied in the application field of OsNPF5.11 gene in improving rice yield, can solve the problems of affecting the growth and development of plants, affecting the growth and development of plants, etc., and achieve the effect of increasing yield

Active Publication Date: 2020-03-24
WUHAN INST OF BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it is known that nitrogen nutrition can promote plant growth and development, there is no systematic understanding of what type of plant growth and development nitrogen nutrition affects.
In addition, the rice NPF family has more than 80 members. It is almost unknown which member of the NPF gene family responds to nitrogen nutrition, and at what site mediates nitrogen nutrition transport, thus affecting the growth and development of the plant.

Method used

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  • Application of osnpf5.11 gene in improving rice yield
  • Application of osnpf5.11 gene in improving rice yield
  • Application of osnpf5.11 gene in improving rice yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 OsNPF5.11 Construction of Gene Overexpression Plants

[0023] Extract the RNA of rice Zhonghua 11 and reverse transcribe it into cDNA, using the primer pair:

[0024] F1: 5'- GGTACC ATGTCGGGGAAGAAGCCGACGGCG-3'( kpn I),

[0025] R1: 5'- TCTAGA GTAGTACTTGCACAAGTGTGTGAA-3'( Xba I);

[0026] Amplified by PCR OsNPF5.11 After cDNA of the gene, passed kpn I. Xba After I digestion, it was connected into the pCAMBIA-1306 vector (the pCAMBIA-1306 vector was purchased from Cambia Company), and the OsNPF5.11 gene overexpression vector OsNPF5.11 -p1306. Agrobacterium EHA105 Mediated genetic transformation method, the overexpression vector was introduced into the normal rice variety Zhonghua 11.

[0027] Transplant all the obtained transgenic seedlings into baskets with soil, water and fertilize them regularly, and plant them in the field when the seedlings grow about 10cm in height. After the seedlings grow up, extract genomic DNA and detect the transge...

Embodiment 2

[0034] Example 2 OsNPF5.11 Construction of Gene Interference Plants

[0035] Extract the RNA of rice Zhonghua 11 and reverse transcribe it into cDNA, using the primer pair:

[0036] F4: 5'- GGTACC AGGCTGAGCAGGGCATGCGTGA-3'( Kpn I),

[0037] R4: 5'- GGATCC TGTAGCCGCCTTGGCCGATGGA-3'( Bam H I);

[0038] F5: 5'- ACTAGT AGGCTGAGCAGGGCATGCGTGA-3'( Speech I),

[0039] R5: 5'- GAGCTC TGTAGCCGCCTTGGCCGATGGA-3'( Sac I);

[0040] PCR amplified OsNPF5.11 After the cDNA fragment of the gene was digested with the corresponding restriction endonuclease, it was connected into the pTCK303 vector to construct the OsNPF5.11 gene interference expression vector OsNPF5.11 -pTCK303. Agrobacterium EHA105 Mediated genetic transformation method, introducing the interference expression vector into the normal japonica rice variety Zhonghua 11.

[0041] Transplant all the obtained transgenic seedlings into baskets with soil, water and fertilize them regularly, and plant them in ...

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Abstract

The invention discloses application of an OsNPF5.11 gene to improvement in rice yield and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF5.11 gene encoding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Through constructing a rice OsNPF5.11 gene overexpressing plant and an OsNPF5.11 gene interference plant, the increase in the normal number of tillers of rice and the number of grouting grains per plant through improving the expression of the OsNPF5.11 gene can be discovered; therefore, the OsNPF5.11 gene can be used in rice breeding to improve the rice yield. The OsNPF5.11 gene has an important application value in aspects of elaborating the influence of nitrogen elements on plant growth and development process and improving the rice plant type.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to OsNPF5.11 Application of genes in improving rice yield. Background technique [0002] Plants obtain nitrogen by absorbing ammonia, nitrate, amino acids, soluble peptides, etc. in the soil; nitrogen absorption and transport mainly rely on ammonium transport protein (AMT), nitrate transport protein (NRT), amino acid transport protein (AAT) , Peptide Transporter (PTR) and other transport proteins (Williams L, Miller A. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annual Review of Plant Physiology and Plant Molecular Biology, 2001, 52: 659-688.). Ammonium is absorbed by plant AMT to synthesize glutamine and glutamate by glutamine synthase (GS) and glutamate synthase (GOGAT), which in turn form other amino acids (Sonoda Y, Ikeda A, Saiki S , et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 方中明黄玮婷汪杰吕凯
Owner WUHAN INST OF BIOENG
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