Gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of gene GauRev2

A technology for Verticillium wilt and cotton, which is applied to the gene GauRev2 that significantly improves the resistance of cotton Verticillium wilt and its application field, and can solve the problems of lack of previous research and unusable breeding
CN113234729AActive Publication Date: 2021-08-10NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2021-08-10

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Abstract

The invention discloses a gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of the gene GauRev2. The gene has a nucleotide sequence as shown in SEQ ID NO. 1. According to the invention, the expression of the GausREV2 gene in cotton is inhibited through a virus-mediated gene silencing technology, and after a verticillium wilt spore suspension is inoculated, the silencing plant is more susceptible to diseases. In arabidopsis thaliana, resistance to verticillium wilt can be remarkably improved through heterologous overexpression of the GausREV2. Discovery and utilization of the GausREV2 are beneficial to cultivation of cotton varieties resistant to verticillium wilt, and the problems in cotton production are solved. The molecular mechanism of the GausREV2 on verticillium wilt resistance is deeply researched, so that the GausREV2 has important theoretical significance for breeding cotton varieties with high verticillium wilt resistance by cotton breeders.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering breeding, and in particular relates to a gene GauRev2 capable of significantly improving cotton Verticillium wilt resistance and an application thereof. Background technique

[0002] Cotton Verticillium wilt is a soil-borne fungal disease caused by Verticillium dahlia. When environmental conditions are suitable, continuous cropping of cotton often results in large-scale occurrence of Verticillium wilt (Atallah and Subbarao, 2012). The pathogenic part is mainly in the vascular tissue of the plant, and once the disease occurs in cotton, it cannot be controlled; more importantly, Verticillium dahliae has strong biological adaptability, and it can survive under low temperature (10°C) and high temperature (30°C) conditions. All can grow, and under adversity conditions, they will also produce dormant structures of micro-sclerotia to avoid adverse environments (Jian Guiliang et al., 2003, Zhu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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