Application of nitrogen transport gene OsNPF7.4 to rice selecting breeding

A rice and gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems affecting plant growth and development, and what type of plant growth and development is affected, so as to enhance tillering ability, increase the number of tillers, and increase plant yield. Effect
CN106947777AActive Publication Date: 2017-07-14WUHAN INST OF BIOENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN INST OF BIOENG
Publication Date
2017-07-14

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Abstract

The invention discloses application of nitrogen transport gene OsNPF7.4 to rice selecting breeding and belongs to the field of plant genetic engineering. An amino acid sequence of an OsNPF7.4 gene coding protein is shown as SEQ ID NO.1, and a cDNA sequence is shown as SEQ ID NO.2. Normal rice tiller number, spike number and grouting grain number can be increased by reducing OsNPF7.4 gene expression after a rice OsNPF7.4 gene interfering plant and an OsNPF7.4 gene over-expression plant are built, therefore, the OsNPF7.4 gene can be applied to rice selecting breeding to improve rice yield. The OsNPF7.4 gene has important application value in the aspect of elaborating the influence of nitrogen transport to plant growing and developing processes.
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Description

technical field

[0001] The invention belongs to the field of plant genetic engineering, in particular to nitrogen transport genes OsNPF7.4 Application in rice breeding. Background technique

[0002] Plants obtain nitrogen by absorbing ammonia, nitrate, amino acids, soluble peptides, etc. in the soil; nitrogen absorption and transport mainly rely on ammonium transport protein (AMT), nitrate transport protein (NRT), amino acid transport protein (AAT) , Peptide Transporter (PTR) and other transport proteins (Williams L, Miller A. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annual Review of Plant Physiology and Plant Molecular Biology, 2001, 52: 659-688.). Ammonium is absorbed by plant AMT to synthesize glutamine and glutamate by glutamine synthase (GS) and glutamate synthase (GOGAT), which in turn form other amino acids (Sonoda Y, Ikeda A, Saiki S , et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in ric...

Claims

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