Composite microbial preparation as well as preparation method and application thereof

A technology of compound microorganisms and microorganisms, which is applied in the field of compound microbial preparations and its preparation, can solve problems such as complex and cumbersome formulas, cumbersome preparation processes, and complex ratios of microbial preparations, and achieve the effects of reducing production costs and improving fermentation efficiency

Active Publication Date: 2018-01-19
TIANJIN BIOFEED TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN105685472 A relates to a preparation and application method of a micro-storage additive, and the micro-storage additive consists of Bacillus licheniformis, Bacillus subtilis, Bifidobacterium bifidum, Enterococcus lactic acid, Lactobacillus acidophilus, Lactobacillus casei, lactic acid Lactobacillus, Lactobacillus plantarum, Pediococcus lactis, Pediococcus pentosacea, Candida utilis, Saccharomyces cerevisiae and Rhodopseudomonas palustris, the formula is complex and cumbersome, and the crude protein content is only increased by 6%
[0007] Therefore, the microbial preparations used in the current biological treatment method are complex in proportion and the preparation process is cumbersome, and the conversion utilization rate and processing time of the produced feed still need to be optimized and improved.

Method used

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  • Composite microbial preparation as well as preparation method and application thereof
  • Composite microbial preparation as well as preparation method and application thereof
  • Composite microbial preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Screening of Bacillus subtilis

[0058] The straws are kneaded and crushed to 10-20 mm, water is added to adjust the water content to 60%-70%, and natural fermentation is carried out for 3 days. Weigh 5 g of naturally fermented straw, add 45 mL of 0.85% physiological saline, and shake for 30 minutes.

[0059] Enrichment and separation of Bacillus: Take 5 mL of supernatant, add it to a sterile test tube, and bath in water at 80°C for 20 minutes. Inoculate 10% of the inoculum into LB liquid medium, and enrich in a shaker at 180r / min and 30°C for 24h. Perform a gradient dilution of the enriched culture solution, select 10 -4 , 10 -5 , 10 -6 The gradient was spread on the LB agar medium, 3 parallels for each dilution, cultured at 37°C for 24h to obtain a single colony, and the single bacteria were picked and stored in the LB slant medium.

[0060] Screening and identification of cellulase-producing bacillus: Using the plate streaking method, select strains from the inc...

Embodiment 2

[0061] Example 2 Screening of Lactobacillus

[0062] The straws are kneaded and crushed to 10-20 mm, water is added to adjust the water content to 60%-70%, and natural fermentation is carried out for 3 days. Weigh 5 g of naturally fermented straw, add 45 mL of 0.85% physiological saline, and shake for 30 minutes.

[0063] Enrichment and separation of lactic acid bacteria: draw 5 mL of supernatant, add it to a sterile test tube, and bath in a water bath at 80°C for 20 minutes. It was inoculated into MRS liquid medium at 10% inoculum, and enriched on a shaker at 180r / min and 30℃ for 24h. Perform a gradient dilution of the enriched culture solution, select 10 -4 , 10 -5 , 10 -6 The gradient was spread on MRS agar medium, 3 parallels for each dilution, and anaerobic culture at 37°C for 24h to obtain a single colony. According to the difference of colony morphology, various typical colonies were picked, and four-zone streaking was performed on another MRS solid plate, and cultured ana...

Embodiment 3

[0066] Example 3 Screening of Saccharomyces cerevisiae

[0067] The straws are kneaded and crushed to 10-20 mm, water is added to adjust the water content to 60%-70%, and natural fermentation is carried out for 3 days. Weigh 5 g of naturally fermented straw, add 45 mL of 0.85% physiological saline, and shake for 30 minutes.

[0068] Enrichment and separation of yeast: Take 5 mL of supernatant, add it to a sterile test tube, and bath at 80°C for 20 minutes. It was inoculated into YPD liquid medium at 10% inoculum amount, and enriched on a shaker at 180r / min and 30°C for 24h. Perform a gradient dilution of the enriched culture solution, select 10 -1 , 10 -2 , 10 -3 The gradient was spread on YPD agar medium, 3 parallel for each dilution, and cultured at 30°C for 24h to obtain a single colony. According to the difference of colony morphology, various typical colonies were picked, and four-zone streaking was performed on another YPD solid plate, and cultured anaerobic at 30°C for 24h...

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Abstract

The invention discloses a composite microbial preparation as well as a preparation method and an application thereof. The composite microbial preparation comprises Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae. The composite microbial preparation is used for fermentation process of microbial silage forage, and can be used for effectively improving fiber degradation rate and biotransformation rate of the forage, improving NDF digestibility, increasing crude protein content, inhibiting breeding of harmful bacterium, and shortening fermentation period with wide application prospects and market values.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and relates to a composite microbial preparation and a preparation method and application thereof. Background technique [0002] Crop stalks are by-products in the production of food crops and cash crops. The main components are cellulose and lignin. They are rich in nitrogen, phosphorus, potassium and other components. They are a high-quality resource for development. my country is a large agricultural country, producing 600 to 700 million tons of straw every year, but the utilization rate is extremely low. According to statistics from the Food and Agriculture Organization of the United Nations (FAO), only 6-8% of my country's meat is transformed from grass and straw, and the conversion ratio is much lower than that of the United States and Australia. my country has a large population and small land, and grain is in short supply for a long time, and feed grain accounts for 35% of tot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/02A23K10/18C12R1/125C12R1/25C12R1/865
Inventor 邓雪娟刘世杰刘晶晶蔡辉益王春雨李宗平程善燕
Owner TIANJIN BIOFEED TECH CO LTD
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