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Fiber degradation strain and enzyme production method thereof

A strain and fiber technology, applied in the fields of agricultural resources and environmental microbes, can solve problems such as inability to decompose sugarcane leaves

Active Publication Date: 2021-08-27
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the harvest season, the temperature is low, and general microorganisms cannot quickly and completely decompose sugarcane leaves under colder climate conditions

Method used

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  • Fiber degradation strain and enzyme production method thereof
  • Fiber degradation strain and enzyme production method thereof
  • Fiber degradation strain and enzyme production method thereof

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Embodiment Construction

[0020] The present invention will be further described in detail below in conjunction with the examples.

[0021] A fiber-degrading bacterial strain and its enzyme-producing method, specifically: a strain of Ochrobactrum intermedium XW2020005, which is isolated from sugarcane leaf decomposing materials, and whose preservation registration number is CGMCC No.20427, and the preservation time is August 14, 2020 day.

[0022] The colony and morphological characteristics of the strain are as follows: the colony on the NA solid medium is milky white, round, smooth and raised, with neat edges and no pigmentation. It is a Gram-negative bacterium with an average size of long (0.8-1.6 ) μm × (1.0-2.4) μm.

[0023] The 16S rDNA sequence of the strain has been registered in GenBank with accession number MW538324.

[0024] The 16S rDNA sequence phylogenetic tree of the strain showed that the strain was highly homologous to Ochrobactrum intermedium.

[0025] The liquid culture of the str...

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Abstract

The invention discloses a fiber degradation strain and an enzyme production method thereof, and particularly relates to Ochrobactrumintermedium XW2020005 which is separated from a sugarcane leaf decomposition material. The preservation registration number is CGMCC No.20427. The strain has the advantages that the strain is separated from a sugarcane leaf decomposition material, can generate filter paper enzyme activity, endoglucanase, beta-glucosidase and other cellulase in the growth and reproduction process, and can decompose sugarcane leaves and other straw in a low-temperature environment.

Description

technical field [0001] The invention relates to the technical field of agricultural resources and environmental microorganisms, in particular to a fiber-degrading bacterial strain and an enzyme-producing method thereof. Background technique [0002] The sugar industry is one of the important economic pillar industries in Guangxi, and it is a "100-billion-billion-dollar industry" that Guangxi focuses on developing. In recent years, Guangxi has a sugarcane planting area of ​​about 1 million ha, and its sugar production has ranked first in the country for more than 20 consecutive years, accounting for more than 60% of the country's total sugar production. It plays a pivotal role in my country's sugar production and safety. [0003] Leave a large amount of sugarcane leaves (including leaf sheaths, etc.) after the sugarcane is harvested, and the output of sugarcane leaves is about 6-10t / ha, so the annual output of sugar cane leaves in Guangxi alone can reach 600-10 million tons. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/01
CPCC12N1/20C12N9/2437C12N9/2445C12Y302/01021C12Y302/01004C12Y302/01091
Inventor 史国英胡春锦曾泉叶雪莲
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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