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Sporotrichum pulverulentum strain and application thereof

A sporozoite and powdery technology, which is applied in the application field of microbial technology, can solve the problems of waste of available resources, ecological environment pollution and the like, and achieves the effects of wide application range and low production cost.

Inactive Publication Date: 2017-02-15
烟台启元微生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these wastes are discarded as garbage or discharged into the environment, resulting in waste of available resources and pollution of the ecological environment.

Method used

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  • Sporotrichum pulverulentum strain and application thereof
  • Sporotrichum pulverulentum strain and application thereof
  • Sporotrichum pulverulentum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation, screening and identification of strains.

[0036] 1. Separation and screening.

[0037] Use the gradient dilution method to dilute the humus soil in the mountains, take 0.1ml and evenly spread it on the surface of the PDA ~ aniline blue medium, put it in a constant temperature incubator, and cultivate it in the dark at 30°C for 10 days, and record the blue medium every day. There is no formation of fading circle and the size of the diameter of the fading circle. Use an inoculation loop to pick a single colony with a large fading circle, and then separate and purify it to be the primary screening strain. Pick 32 single colonies in total, and name the one with the largest fading circle as QY -001. Described PDA aniline blue medium formula is potato 200 g, glucose 20 g, potassium dihydrogen phosphate 3 g, magnesium sulfate heptahydrate 1.5 g, water 1000ml, aniline blue 0.1 g, pH 7.0.

[0038] 2. Identification.

[0039] (1) Morphological charac...

Embodiment 2

[0043] Example 2 Dispospora powderylum QY-001 Strain cultivation method

[0044] 1. Solid plate culture: inoculate the powdery Dispospora QY-001 strain on the wort plate medium and activate it by streaking, then place it upside down in a constant temperature incubator at 30°C for activation culture, and the culture time is 3 to 4 days;

[0045] The formula of the wort plate culture medium is as follows: add 700ml of water to 300g of barley malt powder, take a water bath at 30-40°C for 30 minutes, then take a water bath at 65°C for 3-4 hours, stir continuously during this period, and filter with two layers of gauze to obtain 7-12.5 Wort of Baumedo. Wort juice 500ml, water 500ml, pH5.5, agar 21g, sterilization temperature 121℃, sterilization time 20-30min;

[0046] 2. Liquid shake flask culture: Inoculate the activated bacteria in the solid plate into the liquid medium, and culture it statically at 30°C for 4-10 days, with a liquid volume of 20-30ml / 250ml; or shake it ...

Embodiment 3

[0048] Example 3 Dispospora powderylum QY-001 Application in solid-state fermentation of agricultural waste mixed with a small amount of nitrogen-containing auxiliary materials.

[0049] The whole process includes solid-state fermentation and detection, solid-state fermentation includes seed preparation and agricultural waste fermentation.

[0050] 1. Solid state fermentation.

[0051] (1) Seed preparation: strain activation → first-level liquid seed preparation → second-level solid seed preparation, the specific steps are as follows:

[0052] (a) Strain activation: inoculate Disposporum powder QY-001 on the wort plate and streak for activation, then place it upside down in a constant temperature incubator at 30°C for 3 to 5 days of activation culture; the wort culture medium Prescription is consistent with embodiment 2.

[0053] (b) First-level liquid seed preparation: inoculate the activated bacteria in the wort plate into the liquid medium, and culture it statica...

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Abstract

The invention discloses a novel unique Sporotrichum pulverulentum strain QY-001 wich an accession number of CGMCC No. 10762, and application of the strain to solid-state fermentation of agriculture waste. The Sporotrichum pulverulentum strain QY-001 is screened out from humus soil of a mountain and has strong fiber degradation and utilization effect on agriculture waste; and through solid-state fermentation, the strain enables the content of crude fiber in agriculture waste to be reduced by 40 to 90% and the content of crude protein to be increased by 30 to 300%, so an approach is opened up for a novel biological feed source in China. The Sporotrichum pulverulentum strain QY-001 is simple in nutritional requirements; a fermentation medium is low in production cost; a solid-state fermentation process is simple; and the strain has wide application prospects.

Description

technical field [0001] The invention belongs to the field of microbial technology application, specifically relates to a powdery Disposporium strain and its application in transforming agricultural waste into biological feed, and belongs to the technical category of waste recycling in circular agriculture. Background technique [0002] Agricultural waste refers to the general term for organic substances discarded by humans in the process of organizing agricultural production, mainly including (1) plant waste, mainly referring to crop straw, forestry branches, weeds, fallen leaves, fruit shells, etc., my country About 1 billion tons of planting wastes are produced every year; (2) Wastes from agricultural and sideline product processing, such as dregs, potato residues, slaughtered blood, etc.; (3) Animal wastes, such as livestock and poultry manure, etc., my country Every year, about 2.6 billion tons of livestock and poultry manure are produced; (4) Domestic waste of rural resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K10/18C12R1/645
Inventor 况代武
Owner 烟台启元微生物技术研究院有限公司
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