Novel bromelain preparation and preparation method thereof

A technology of bromelain and preparations, applied in the field of protease preparations, which can solve problems such as inability to realize sustained and controlled release of enzymes, affect application, and be difficult to remove, so as to solve environmental pollution and waste of resources, prolong storage period and use period, and reduce costs. cheap effect

Pending Publication Date: 2017-12-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this invention adopts glutaraldehyde as cross-linking agent, and glutaraldehyde is commonly used as bactericide, has strong stimulation to glasses, skin and mucous membrane and is difficult to remove, thus has influenced the application of this invention in food industry; It is necessary to adopt an additional process on the basis of the separation and purification process of bromelain; and, because the enzyme preparation of the invention cannot realize the slow release and controlled release of the enzyme, this also limits the enzyme preparation prepared by the invention in the larger viscosity Applications in liquid and solid food

Method used

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  • Novel bromelain preparation and preparation method thereof

Examples

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Embodiment 1

[0041] Take 100g pineapple peel, wash it, add 100mL deionized water, squeeze the juice, filter it with nylon cloth, adjust the pH value of the clear liquid to 3.0 with citric acid, add an equal volume of 1% chitosan solution, stir and mix evenly. Place in a refrigerator at 4°C for 24 hours, centrifuge at 10,000 r / min for 20 minutes, take the supernatant, and dilute to 100 mL with deionized water to obtain 10% pineapple juice. Store in refrigerator at 4°C for later use.

[0042] Dilute 2% (w / v) sodium alginate solution to 0.5% (w / v) with deionized water, adjust 10% pineapple juice and 0.5% (w / v) sodium alginate solution with citric acid solution When the pH value reached 3.0, the two solutions with the same pH were mixed in equal volumes, stirred at 4°C for 30 minutes, centrifuged at 4000 r / min for 20 minutes, and the bromelain-sodium alginate polyelectrolyte complex precipitate was collected and freeze-dried to obtain a bromelain preparation. Under this condition, the recover...

Embodiment 2

[0045] Take 100g pineapple peel, wash it, add 100mL deionized water, squeeze the juice, filter it with nylon cloth, adjust the pH value of the clear liquid to 2.5 with malic acid, add an equal volume of 1% chitosan solution, stir and mix evenly. Place in a refrigerator at 4°C for 24 hours, centrifuge at 10,000 r / min for 20 minutes, take the supernatant, and dilute to 100 mL with deionized water to obtain 10% pineapple juice. Store in refrigerator at 4°C for later use.

[0046] Dilute the 2% (w / v) pectin solution to 1% (w / v) with deionized water, adjust the pH value of 10% pineapple juice and 1% (w / v) pectin solution with malic acid solution to 2.5, then equal volumes of the two solutions with the same pH were mixed, stirred at 35°C for 10 minutes, centrifuged at 4000r / min for 20 minutes, and the bromelain-pectin polyelectrolyte complex precipitate was collected and vacuum-dried to obtain a bromelain preparation. Under this condition, the recovery rate of the enzyme activity i...

Embodiment 3

[0049] Take 100g pineapple peel, wash it, add 100mL deionized water, squeeze the juice, filter it with nylon cloth, adjust the pH value of the clear liquid to 4.0 with citric acid, add an equal volume of 1% chitosan solution, stir and mix evenly. Place in a refrigerator at 4°C for 24 hours, centrifuge at 10,000 r / min for 20 minutes, take the supernatant, and dilute to 100 mL with deionized water to obtain 10% pineapple juice. Store in refrigerator at 4°C for later use.

[0050] Dilute a 2% (w / v) sodium carboxymethylcellulose solution to 1% (w / v) with deionized water, and dilute 10% pineapple juice and 1% (w / v) sodium carboxymethylcellulose The pH value of the solution was adjusted to 4.0 with citric acid solution, and then the two solutions with the same pH were mixed in equal volumes, stirred at 40°C for 20 minutes, centrifuged at 4000r / min for 20 minutes, and the bromelain-sodium carboxymethylcellulose polyelectrolyte complex precipitate was collected , vacuum-dried to obta...

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Abstract

The invention discloses a preparation method of a novel bromelain preparation. The preparation method comprises the following steps: (1) preparing pineapple juice; (2) preparing a natural polyanion polysaccharide solution; (3) respectively adjusting the pineapple juice and the natural polyanion polysaccharide solution until the pH values are the same, adequately mixing the pineapple juice with the natural polyanion polysaccharide solution, and stirring to generate a bromelain-polyanion polysaccharide compound precipitate through an electrostatic attraction interaction between the bromelain and polyanion polysaccharide in the pineapple juice; and (4) centrifugally collecting the bromelain-polyanion polysaccharide compound precipitateand drying, so as to obtain the bromelain preparation. By utilizing a principle of generating the precipitate by virtue of the electrostatic attraction interaction between the bromelain and polyanion polysaccharide, the bromelain is separated, and the bromelain preparation is obtained. The bromelain preparation has the stability of bromelain in storage and processing processes and has good slow-release and control-release properties, and the disadvantages that bromelain is sensitive to head and easy to oxidize can be overcome.

Description

technical field [0001] The invention belongs to the technical field of protease preparations, and in particular relates to a novel bromelain preparation and a preparation method thereof. Background technique [0002] Bromelain is a kind of protease extracted from pineapple peel and pineapple stem. The production of bromelain can solve the problems of environmental pollution and resource waste caused by a large amount of waste discarded in the pineapple industry, and can also make high-value utilization of pineapple products. Bromelain belongs to the papain enzyme system, and is divided into stem bromelain (EC3.4.22.32) and fruit bromelain (EC3.4.22.33) according to the different parts of the separation and extraction. The molecular weights of the two are 33kDa and 28kDa, respectively, and the isoelectric point 9.5 and 4.6, respectively, commercial bromelain mostly stem enzymes. Bromelain has a wide range of applications in the pharmaceutical and food industries. In the fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/96
Inventor 黄国清肖军霞孙欣郭丽萍
Owner QINGDAO AGRI UNIV
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