1 results about "Molecular surfaces" patented technology

The invention discloses a preparation method of nano-sized hydrogen bond organic framework immobilized carbonic anhydrase particles, which comprises the following steps: when a carbonic anhydrase (CA) solution and an HOF monomer (1, 3, 6, 8-tetra (4-carboxybenzene) pyrene) solution are blended, mixing an organic phase and a water phase under stirring so that monomers surround CA to form condensation nucleuses, and inducing the monomers to self-assemble into the nano-sized immobilized enzyme particles. The hydrogen bond organic framework immobilized enzymes with different sizes and structures are prepared by regulating and controlling the ligand concentration, the enzyme concentration and the dripping speed. HOF in-situ nucleation and growth are induced on the surface of an enzyme molecule to form a confinement space protection layer, so that the enzyme has more excellent heat resistance, acid and alkali resistance and operation stability; due to the high porosity, the structure adjustability and the excellent mass transfer performance of the HOF, the performance of CA in the aspects of catalysis, separation and cyclic utilization is enhanced, and particularly the stability under extreme pH and temperature changes is enhanced. The method is simple and feasible in process, mild in condition and suitable for construction of a series of hydrogen bond organic framework immobilized enzymes.