38results about How to "Avoid freeze damage" patented technology

The invention discloses a cold and hot probe device and particularly relates to a probe device for cold and hot combination therapy by electric heating and liquid nitrogen refrigerating, which is suitable for treating various solid tumors. The cold and hot probe device comprises a low-temperature pressure vessel, a low-temperature working medium, a booster, a low-temperature fluid valve, a low-temperature working medium transport tube, a handle, a probe pole, a probe head, an electric heating element, a temperature sensor, a data acquisition/control unit, a DC power supply module, a computer and the like. The invention can cool and heat the probe head conveniently so as to implement the cold and hot combination treatment and prevent normal tissues around the probe pole from being frozen and damaged in the cooling process. The invention has the advantages of simple structure, low cost, good controllability, high safety, convenient use and the like.

The invention discloses an icing detection method and device for an air conditioner indoor unit, and belongs to the technical field of air conditioners. The icing detection method comprises the stepsthat the outdoor environment temperature and the suction temperature of a compressor when an air conditioner operates in a refrigeration mode or a dehumidification mode are acquired; and when the outdoor environment temperature and the suction temperature meet preset icing judgment conditions, the air conditioner is triggered to enter an icing protection mode, wherein the icing conditions refer tothat the outdoor environment temperature is higher than or equal to a first outdoor temperature threshold value and the suction temperature is smaller than or equal to a first suction temperature threshold value. According to the icing detection method for the air conditioner indoor unit, whether or not the indoor unit has the icing tendency can be judged according to outdoor unit relevant parameters such as the outdoor environment temperature and the suction temperature of the compressor, and corresponding protection is triggered in time, so that the problem of detection failures caused whenan indoor unit coil is affected by the factors such as refrigerant deficiency or indoor distribution variations is avoided, and the phenomenon that the air conditioner indoor unit is damaged due to icing is avoided.

The invention relates to a three-dimensional aerial fog seedling method for flue-cured tobacco, and belongs to the technical field of seedling of flue-cured tobacco. The seedling method comprises the following steps: (1) making a cultivation pond; (2) making a seedling bracket; (3) making a cultivation plate; (4) sowing; (5) making a liquid storing pond; (6) preparing a nutrient solution; (7) arranging an automation circulating system; (8) managing a seedbed. According to the seedling method, the temperature and the moisture can be controlled easily, high automatic control rate is realized, water can be saved, the fertilizer water utilization ratio is increased, and the cost can be effectively reduced when the method is popularized to drought regions; high absorption ability of nutrient is realized, and tobacco seedlings grow rapidly and robustly; a supplementary lighting measure is not needed, so that the seedling cost is reduced in comparison to multilayer seedling. A three-dimensional aerial fog seedling facility is suitable for planting crops such as vegetables and flowers, the comprehensive utilization ratio of a seedling greenhouse is increased greatly, and the income is increased effectively.

The invention discloses a peripheral blood mononuclear cell serum-free freezing medium. The peripheral blood mononuclear cell serum-free freezing medium is prepared from the following components in percentage by volume: 5 to 20% of dimethyl sulfoxide, 0.5 to 10% of dextran 40, and the balance of plasmalyte A. The invention further discloses a method for freezing peripheral blood mononuclear cells through the peripheral blood mononuclear cell serum-free freezing medium. Compared with the prior art, the freezing medium is free of animal serum, human serum and a cell culture medium, and has a good freezing effect; the cells can be directly applied to the clinic after resuscitation and can be also induced in vitro into immune cells NKT and CIK and have high application value in the clinic.

The invention provides a method for preparing a direct-vat-set(DVS) starter of lactic acid bacteria at normal temperature and aims to provide a method for preparing a DVS starter of lactic acid bacteria, which has the advantages of simple preparation process, low cost, high biomass and activity. The method comprises the following steps: applying lactose and glycerin on peeled and chopped steamed breads as a protectant, sterilizing, drying and using the peeled and chopped steamed breads as carriers; coating the bacterial suspension of lactic acid bacteria on the steamed bread carrier under a sterile condition to obtain the starter of lactic acid bacteria coated with the strain; and drying the starter of lactic acid bacteria coated with the strain by blowing at normal temperature and under the sterile condition to obtain the DVS starter of lactic acid bacteria. In the method, the lactose and the glycerin are applied on the steamed bread carriers as the protectant in the drying process of the steamed breads; simultaneously starch and proteins in the steamed breads can also well protect the lactic acid bacteria and the frozen damage is avoided; therefore, the starter is quite high in bacterial survival rate, activity and biomass. In addition, the method also has the advantages of simplicity, convenience for implementation and low cost.

The invention belongs to a qualitative detection technology of plant derived components in the foods and the beverages, in particular to a primer/ a probe group for detecting a carrot component in the foods and the beverages. In the invention, a group of specific primer and probe are designed aiming at antifreeze protein of the carrot and according to the conserved sequence of a gene thereof. The carrot component in the foods and the beverages can be rapidly, sensitively and specifically detected by using the primer and the probe and adopting a real-time fluorescence PCR technology. The primer and the probe can be provided with other reagents together in the form of a kit and are used for amplification reaction of nucleic acid. The method has simple operation and good repeatability.

The invention belongs to a technique for qualitatively detecting plant-derived components in foods and beverages, in particular to a kit and a method for detecting carotene components in the foods and the beverages by using a real-time fluorescent PCR (Polymerase Chain Reaction) technique. The kit comprises a PCR amplification reactive solution A, UNG (unguentum) enzyme B and Taq enzyme C, wherein the PCR amplification reactive solution A contains 10*PCR reaction buffers, magnesium sulfate, dNTP (deoxy-Nucleotide Triphosphate) containing dUTP (deoxy-Uridine Triphosphate), an upstream primer of 5'-CGACAAGCAAGCTTTACTCCAA-3', a downstream primer of 5'-CGTCTGACACCCATGAGTCTGT-3' and a TaqMan probe 5'-FAM-TCAAAACAGCCTTGAAAAACCCCACCA-TRAMA-3'. The method for rapidly detecting carotene components comprises the steps of extraction of DNA (Deoxyribonucleic Acid) in the foods and beverages, real-time PCR amplification of the carotene components and result judgement. The method has the advantages of rapidity, strong specificity, high sensitivity, convenient operation and good repeatability.

The present invention belongs to the field of marine biotechnology, and is technology of obtaining normal revived genuine porgy embryo including vitrification freezing of embryo, long period maintenance in liquid nitrogen, deforesting, eluting, etc. The genuine porgy embryo in palpitation stage is treated through adding antifreezing agent, fast straw vitrification freezing, maintenance in liquid nitrogen, deforesting, eluting and other steps to obtain the revived genuine porgy embryo. The present invention is significant in the germ plasm maintenance, genetic diversity, breeding, etc of genuine porgy.

The invention relates to an immune cell cryopreservation method. The immune cell cryopreservation method comprises the following steps: immune cell cryopreservation liquid is prepared, wherein the immune cell cryopreservation liquid is prepared from the following raw materials in percentage by weight: 3-7 mL of dimethyl sulfoxide, 2-6 mL of dextran, 15-25 mL of a cell culture medium, 2-4 mL of human serum albumin, 2-4 mL of raffinose, 2-4 mL of glucose, 1-3 mL of propylene glycol and 60-80 mL of human autologous plasma; and immune cells are cryopreserved through the immune cell cryopreservation liquid. Compared with the prior art, the immune cells are effectively protected against freezing damage, safety is high, damage to the cells is small, and the recovery survival rate of the immune cells can be increased and can reach 96% or above.

The invention relates to a Hanfu apple short-stem high-crown planting method, which comprises the following process steps of: 1, selecting a piece of land; 2, performing land preparation; 3, digging planting ditches, wherein the depth of the ditches is 0.8 meter, the width of the ditches is 0.6 to 0.7 meter, the depth of dug holes is 0.8 meter, the diameter of the dug holes is 1 meter, the dug holes are circular or square, and surface soil and subsoil are stacked respectively so as not to make soil layers confused; 4, performing land preparation in autumn or before winter; and 5, after digging the planting ditches (holes), spreading a layer of deadwood and disordered leaves such as straws or weeds on the ditches, backfilling the subsoil for settling, backfilling the surface soil for ridging, and planting apple nursery stocks in ridges which is 0.5 to 0.6 meter higher than the ground. The apple tree planting method has the advantages of improving the illuminated area of planted nursery stocks, improving the utilization rate of sunlight, increasing ground temperature and making the root systems of the nursery stocks quickly recover calli to absorb nutrients.

The invention discloses a preparation method of a yogurt starter culture, and belongs to the technical field of foods. According to the invention, fruit juice is added, and various fruits contain components which are beneficial to the human bodies so as to further increase the efficacy of yogurt. Meanwhile, various sugars are added into a nutrient solution, and a sugar content is high so as to notonly provide nutrient fermentation with a better nutrient environment and improve the activity of strains, but also accelerate a fermentation rate. Besides, the two strains adopted by the invention promote the growth of all parties. Meanwhile, defatted milk powder and an antioxidant are added to form a carrier protective agent, so that the carrier protective agent can enter the interior of cellsand prevent freezing injuries inside and outside the cells. A mixture is subjected to pre-freezing, and gets into a solid state so as to avoid the inactivation of active macromolecules in a process ofvacuum freeze-drying and maintain the original characteristics of the strains. The method solves problems of some existing yogurt starter cultures that a flavor is not heavy enough and sourness is too strong, which result in a poor mouth feel and the need of adding sweeteners.

The invention discloses a comprehensive management and cultivation method for promoting rapid propagation of poplar seedlings. The comprehensive management and cultivation method comprises the following steps: (1) selecting sandy loam or light loam with flat terrain and loose soil as a seedling nursery field, applying base fertilizer, deep ploughing, flattening, sterilizing, watering, air-drying, shallow hoeing and loosening the seedling nursery field; (2) after land is unfrozen at the beginning of spring and before the flowing of sap, cutting branches of a one-year poplar seedling trunk which grows strongly and develops well, and treating the branches; (3) vertically cutting inserting the lower end of the branch into loose soil of the nursery field, wherein a distance between two adjacent plants is 0.3 to 0.4 m; and (4) watering once two weeks after the branches are inserted, shallow hoeing and loosening the soil after the watering, and carrying out top dressing once two months. The cultivation method is simple, the cultivation period is short, and the rapid propagation of the poplar seedlings can be promoted.