The invention provides a method for preparing a direct-vat-set(DVS)
starter of
lactic acid bacteria at normal temperature and aims to provide a method for preparing a DVS
starter of
lactic acid bacteria, which has the advantages of simple preparation process, low cost, high
biomass and activity. The method comprises the following steps: applying
lactose and glycerin on peeled and chopped steamed breads as a protectant, sterilizing,
drying and using the peeled and chopped steamed breads as carriers;
coating the bacterial suspension of
lactic acid bacteria on the
steamed bread carrier under a sterile condition to obtain the
starter of lactic acid bacteria coated with the strain; and
drying the starter of lactic acid bacteria coated with the strain by blowing at normal temperature and under the sterile condition to obtain the DVS starter of lactic acid bacteria. In the method, the
lactose and the glycerin are applied on the
steamed bread carriers as the protectant in the
drying process of the steamed breads; simultaneously
starch and proteins in the steamed breads can also well protect the lactic acid bacteria and the frozen damage is avoided; therefore, the starter is quite high in bacterial
survival rate, activity and
biomass. In addition, the method also has the advantages of simplicity, convenience for implementation and low cost.