Mononuclear cell frozen stock solution of cord blood, application and preparation method

Abstract

The invention relates to the field of biological technology, and particularly relates to a mononuclear cell frozen stock solution of cord blood, an application and a preparation method. The mononuclear cell frozen stock solution of cord blood provided by the invention comprises dimethyl sulfoxide (DMSO) united plasmalyte force A (compound electrolyte injection) and hydroxyethyl starch (HES). Compared with the traditional formula, the scheme can be used for effectively preventing the pollution of animal derived pathogens and avoiding using of a cell culture medium to serve as a component of the frozen stock solution and guaranteeing a good cell frozen stock effect; after cell frozen for 2 months, the average cell viability of resurgent CBMC cell frozen by the cell frozen solution is 94.73%, which is remarkably (P is smaller than 0.05) higher than the cell frozen effect of the traditional cell frozen solution (10%DMSO+90%FBS). The resurgent cell can be directly applied to clinical transplantation, so the mononuclear cell frozen stock solution of the cord blood has a large practical value in clinical application of cell therapy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cord blood mononuclear cell cryopreservation solution, application and preparation method. Background technique [0002] Cord blood mononuclear cell (CBMC) refers to cells with a single nucleus in cord blood, including hematopoietic stem cells, lymphocytes, monocytes, dendritic cells and a small number of other cells. CBMC can promote the body's hematopoiesis and enhance the body's immunity in vivo; it can induce differentiation into a variety of immune cells in vitro, such as cytokine-induced killer cells (CIK), natural killer cells (NK), and natural killer T cells (NKT) , has important uses in anti-tumor, anti-infection and other aspects. Therefore, cryopreservation of CBMC is of great significance in cell therapy. [0003] Cell cryopreservation is one of the main methods for long-term preservation of cells. Using cryopreservation technology to store cells in -196°C liquid nit...

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Problems solved by technology

[0006] Cell culture medium is also a common component in the current cryopreservation solution formula. Its components are complex and cannot be directly injected into the human body. Therefore, if cells need to be transplanted directly after recovery, the cell culture medium must be removed by centrifugation, and centrifugation will cause The newly recovered cells are damaged and their viability is reduced, which affects the effect of cell therapy

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