Freezing diluent for seminal fluid of livestock

Abstract

The invention relates to a freezing diluent for a seminal fluid of livestock. Every 100 ml of the freezing diluent comprises components as follows: 2.71 g of trihydroxymethyl aminomethane, 1.4 g of citric acid, 1.0 g of monosaccharide, 5-20 ml of fresh yolk, 0-10 ml of a permeable protecting agent, 0.1 million IU of penicillin, 0.1 million IU of streptomycin, 0.05-5 g of polyvinyl alcohol and the balance of ultrapure water. The common high-molecular polymer polyvinyl alcohol serving as an ice crystal inhibitor replaces natural antifreeze protein and is applied to the cryopreservation research of the seminal fluid of livestock for the first time. The survival rate of unfrozen sperms is about 75%, the activity is 50% or higher, the acrosome completeness is 65% or higher, the plasma membrane completeness is about 50%, and the non-return rate after artificial insemination is 70% or higher.

Description

technical field

[0001] The invention relates to the technical field of animal reproductive physiology and reproduction, in particular to a frozen diluent of livestock semen. Background technique

[0002] Although the research on cryopreservation of livestock semen has been carried out for more than 50 years, the viability of thawed semen is only about 30%-50%, and the non-return rate and litter rate after artificial insemination are significantly lower than that of fresh semen. During the freezing process of livestock semen, the mechanical damage caused by the ice crystals formed inside and outside the cells directly leads to the decline of sperm motility after freezing. How to reduce the damage to livestock sperm produced by ice crystals has always been a research field in the field of livestock breeding technology and cryobiology. Hotspot, by changing the composition of semen diluent, the damage of freezing to sperm can be reduced. At present, osmotic organic solvents and ...

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