The invention belongs to the technical field of sperm acrosome detection, and discloses an improved crystal violet staining method for detecting sperm acrosome. The method comprises the following steps: preparing an improved gelatin film; liquefying collected fresh seminal fluid, subjecting seminal fluid to centrifugation, discarding seminal plasma, suspending precipitates in a phosphate buffer solution, placing the buffer solution in a thermotank, evenly paving sperm suspension on the gelatin film, incubating the sperms in a thermotank with a temperature of 37 DEG C, after 30 minutes, takingout sperm suspension for microscopic examination; dropwise adding a crystal violet dyeing solution on a fixed smear, carrying out dyeing for 1 to 3 minutes, washing the smear by water to remove excessdyes; dropwise adding a Lugol iodine solution, after one minute, washing the smear by water, then dropwise adding alcohol (95%) to perform color separation, shaking the glass slide until the purple color is not faded, washing the smear by flowing water, and carrying out microscopic examination after drying. A simple and rapid dyeing medium adopted by the crystal violet staining method can enhancethe cell dyeing affinity, through a discoloring treatment, the binding strength between a fuel and a polluted object can be measured, the structural difference of cells can be discriminated, and thesituation of sperm acrosome is well displayed.