The invention relates to a fluorescence dye for detecting fungus and dermatozoon. The technical problems that three dyeing liquid ingredients of a current fluorescence dye method cannot stably coexist for a long time, and need to be prepared and stored respectively, operating is tedious, the dyeing time is long, limitation exists in detected samples, some samples with the small impurities can only be dissolved, and the clinical application range is limited are solved. The fluorescence dye is prepared from water, a fluorescent whitening agent, alkali, a background redyeing agent, a chaotropic agent and a moisturizing agent. As the fluorescence dye is a single dosage form, the fluorescent whitening agent, strong alkali and the background redyeing agent can stably coexist; the stability of a product is good, dyeing can be completed only through one step, clinical using is more convenient and fast, original detection which can be completed in 5 minutes or above can be completed in tens of seconds through the fluorescence dye, and the detection efficiency of the fungus and the dermatozoon is greatly improved.