6 results about "Fluorescent stain method" patented technology

The invention belongs to the technical field of aquatic pathogenic microorganism products, and particularly relates to an in-vitro culture method of enterospora hepatopenaei. The method comprises the following steps: obtaining purified spores of enterospora hepatopenaei; the method comprises the following steps: inoculating purified spores of enterospora hepatopenaei into a host cell being cultured for infection to obtain a host cell sample infected with enterospora hepatopenaei; and detecting the proliferation and maturation of the purified spores of the enterospora hepatopenaei in the host cell sample infected with the enterospora hepatopenaei by using a fluorescent staining method. The method provided by the invention provides a key technical platform for basic research, pathogenic mechanism analysis, drug screening and vaccine development of the shrimp enterospora hepatopenaei, has the advantages of convenience in acquisition of experimental materials, simplicity and convenience in operation, high repeatability and the like, and is suitable for in-vitro culture and proliferation of the pathogen in a laboratory.

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The application provides application of a compound shown in formula (III) in multicolor immunofluorescence staining. The method can be used for marking a plurality of different fluorescent dyes on a plurality of antigens in a sample, and then the fluorescent dyes in the last round of staining can be eluted through an elution process, so that a new round of multicolor immunofluorescence marking can be started. The marked sample can be used for imaging by instruments and equipment such as a fluorescence microscope or a laser confocal microscope, and after software synthesis, the sample marked by a plurality of different colors can be displayed. The colors are clear, bright and do not bleed. The application not only retains the sensitivity and staining intensity of the TSA fluorescent staining method, but also overcomes the limitation of the number of multicolor fluorescent markers.

Proposed is a method for estimating the mass of microplastics by using fluorescent staining. The method may include staining microplastic samples by using a Nile red solution, and capturing a fluorescent image of each of the stained microplastic samples after classifying the stained microplastic samples by mass. The method may also include analyzing the captured fluorescent image to quantify a fluorescence intensity thereof to build a database (DB) about a correlation between mass and fluorescence intensity of microplastics. The method may further include estimating a mass value of an unknown microplastic sample by comparing a fluorescence intensity of a fluorescent image of the unknown microplastic sample obtained by performing the staining and capturing of the unknown microplastic sample with data stored in the database.

The present invention provides a fluorescent staining method for live-cell imaging. First, cells are double stained through the first and second fluorescent biomarker. Then, the clear fluorescent cell image is shown under a fluorescent microscope, and we can observe the nucleus form while observing the cell form through the obtained image.

The application belongs to the field of biological detection, and particularly relates to a TUNEL-TSA multiplex fluorescence staining method based on TSA technology optimization and application thereof. Tyramide signal amplification technology (TSA) is used to improve and optimize TUNEL combined immunofluorescence staining. The TSA technology can make the fluorescence signal intensity increase by more than 10 times, while maintaining a more complete cell morphological structure. In the multiplex staining process, the sequential scheme of "antigen retrieval-TSA fluorescence staining-protease K digestion-TUNEL staining" is significantly better than other staining strategies. It provides reliable technical support for establishing a standardized detection system, and has important clinical application and popularization value.