9 results about "Fluorescent stain method" patented technology

The invention belongs to the technical field of aquatic pathogenic microorganism products, and particularly relates to an in-vitro culture method of enterospora hepatopenaei. The method comprises the following steps: obtaining purified spores of enterospora hepatopenaei; the method comprises the following steps: inoculating purified spores of enterospora hepatopenaei into a host cell being cultured for infection to obtain a host cell sample infected with enterospora hepatopenaei; and detecting the proliferation and maturation of the purified spores of the enterospora hepatopenaei in the host cell sample infected with the enterospora hepatopenaei by using a fluorescent staining method. The method provided by the invention provides a key technical platform for basic research, pathogenic mechanism analysis, drug screening and vaccine development of the shrimp enterospora hepatopenaei, has the advantages of convenience in acquisition of experimental materials, simplicity and convenience in operation, high repeatability and the like, and is suitable for in-vitro culture and proliferation of the pathogen in a laboratory.

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The invention belongs to the technical field of biology, and discloses a muscular tissue fixing method suitable for transportation, which comprises the following steps: placing a freshly obtained muscle sample in a formalin solution with the concentration of 8-12wt%, and fixing for 14-18h; and then, immersing the fixed muscle sample in an ethanol solution with the concentration of 60-80 vol% for preservation. According to the method, the tissue sample is fixed by formalin and soaked in an ethanol solution, so that the tissue sample can be stored, preserved and transported for a long time, the subsequent fluorescent staining is not influenced, and in the experiment process, the subsequent fluorescent staining is not influenced even if the tissue sample is preserved for half a year by adopting the method disclosed by the invention. Meanwhile, the invention also provides a fluorescent staining method of the muscle tissue.

The invention relates to the technical field of plant biology, in particular to a fluorescent staining method for dendrobium stigma pollen germination, which comprises the following five steps: collecting dendrobium flowers with stigmas, removing redundant parts such as sepals and the like, only reserving the stigmas, trimming two sides of each stigma into a horizontal shape, putting the stigmas into a precooled stationary liquid for fixing, and then taking out the stigmas; carrying out dehydration treatment by using finished alcohol; cutting the dehydrated stigma along the central line of the stigma by using a scalpel; treating the cut sample in a softening agent to soften the tissue of the sample; transferring the transparent sample into a staining solution containing a fluorescent dye, and staining under a dark condition; and after dyeing is completed, taking out the sample, fixing the sample on a glass slide, covering with a cover glass, and observing the germination condition of the dendrobium stigma pollen under a fluorescence microscope. By means of the method, the germination condition of the dendrobium stigma pollen can be clearly and accurately observed, and powerful technical support is provided for relevant research of dendrobium and plant materials with large stigmas.

The application provides application of a compound shown in formula (III) in multicolor immunofluorescence staining. The method can be used for marking a plurality of different fluorescent dyes on a plurality of antigens in a sample, and then the fluorescent dyes in the last round of staining can be eluted through an elution process, so that a new round of multicolor immunofluorescence marking can be started. The marked sample can be used for imaging by instruments and equipment such as a fluorescence microscope or a laser confocal microscope, and after software synthesis, the sample marked by a plurality of different colors can be displayed. The colors are clear, bright and do not bleed. The application not only retains the sensitivity and staining intensity of the TSA fluorescent staining method, but also overcomes the limitation of the number of multicolor fluorescent markers.

Proposed is a method for estimating the mass of microplastics by using fluorescent staining. The method may include staining microplastic samples by using a Nile red solution, and capturing a fluorescent image of each of the stained microplastic samples after classifying the stained microplastic samples by mass. The method may also include analyzing the captured fluorescent image to quantify a fluorescence intensity thereof to build a database (DB) about a correlation between mass and fluorescence intensity of microplastics. The method may further include estimating a mass value of an unknown microplastic sample by comparing a fluorescence intensity of a fluorescent image of the unknown microplastic sample obtained by performing the staining and capturing of the unknown microplastic sample with data stored in the database.

The present invention discloses a non-washing two-color cell imaging fluorescein carbon dot staining solution for fungal detection, which, by mass percentage, comprises: 0.50% - 1.50% of fluorescein carbon dot material and 98.50% - 99.50% of water; wherein the fluorescein carbon dot material is made from raw materials with the following mass percentages: 0.5% - 1.3% of fluorescent brightener 135#, 25% - 29% of ethylenediamine, 0.05% - 0.13% of ammonium persulfate, and 69.57% - 74.45% of water. Also disclosed is a method for fungal staining using the above staining solution and the application of the above fluorescein carbon dot staining solution in fungal detection using fluorescence staining method. In particular, this staining solution can accurately determine the presence of epidermal fungi without removing dandruff, has simple components, can remain stable for a long time, and greatly reduces the cost.

The present invention provides a fluorescent staining method for live-cell imaging. First, cells are double stained through the first and second fluorescent biomarker. Then, the clear fluorescent cell image is shown under a fluorescent microscope, and we can observe the nucleus form while observing the cell form through the obtained image.

The application belongs to the field of biological detection, and particularly relates to a TUNEL-TSA multiplex fluorescence staining method based on TSA technology optimization and application thereof. Tyramide signal amplification technology (TSA) is used to improve and optimize TUNEL combined immunofluorescence staining. The TSA technology can make the fluorescence signal intensity increase by more than 10 times, while maintaining a more complete cell morphological structure. In the multiplex staining process, the sequential scheme of "antigen retrieval-TSA fluorescence staining-protease K digestion-TUNEL staining" is significantly better than other staining strategies. It provides reliable technical support for establishing a standardized detection system, and has important clinical application and popularization value.