Antifreeze used for freezing and preserving boar semens as well as preparation and application thereof

A technology of cryopreservation and antifreeze, which is applied in application, preservation of human or animal bodies, animal husbandry, etc. It can solve the problems of sperm viability, acrosome integrity and plasma membrane integrity, and the inability of frozen pig semen It is widely used in commercial pig production, and the sperm motility rate is not very ideal, so as to achieve the effect of maintaining insemination vitality, easy promotion and reliable effect

Inactive Publication Date: 2010-08-11
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since porcine sperm is particularly sensitive to cold shock, the viability, acrosome integrity, plasma membrane integrity, and developmental ability of thawed sperm are far inferior to those of fresh semen. Frozen semen cannot be widely us...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of dove egg yolk

[0027] Take fresh pigeon eggs, lightly tap a small opening on the eggshell to remove the egg white, put the yolk covered with a complete yolk membrane on a clean filter paper, use the filter paper to absorb the egg white on the yolk membrane, and then pierce the yolk with a clean syringe Pour the egg yolk into a beaker, stir evenly, divide into centrifuge tubes, centrifuge at 4°C for 45min (10000×g), and take the supernatant for later use.

Embodiment 2

[0029] Dissolve 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate in 100ml of distilled water to prepare a conventional freezing solution; accurately measure 88-90ml of conventional freezing base solution, add fresh pigeon Mix 10-12ml of egg yolk to make liquid I; take 91ml of liquid I and add 9ml of glycerin to make liquid II, adjust the pH of the solution to 6.0-7.5, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.

Embodiment 3

[0031] Dissolve 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate in 100ml of distilled water to make a conventional freezing solution; accurately measure 85-87ml of the conventional freezing base solution, add fresh pigeon Mix 13-15ml of egg yolk to make liquid I; take 91ml of liquid I and add 9ml of glycerin to make liquid II, adjust the pH of the solution to 6.0-7.5, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.

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PUM

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Abstract

The invention discloses an antifreeze used for freezing and preserving boar semens as well as preparation and application thereof. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, contains pigeon egg yolk and concretely comprises the following components in parts by volume: 82 to 90 of freezing base liquid and 10 to 18 of pigeon egg yolk. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, has simple preparation method, easy material acquirement, reliable effect and easy popularization, is suitable for insemination after long-time preservation and long-distance transportation after the artificial insemination of the frozen semens of the tubules of boars and the ultra-low temperature freezing of the semens of the boars, can obviously enhance the survival rate of the semens, the integrity of the acrosomes and the integrity of the plasmalemma after the boar semens are frozen and unfrozen, and keeps the insemination activity of the boar semens.

Description

technical field [0001] The invention relates to an antifreeze agent for pig semen preservation and its preparation and application. Background technique [0002] Since Polge et al. started the experimental research on cryopreservation of pig semen in 1956, many countries have successively carried out research on this technology, with the goal of improving its reproductive performance and reaching an acceptable level for on-site production. Pig farming is an important part of animal husbandry. The normal temperature liquid storage technology of pig semen has been widely promoted and applied to pig production practice. Semen cryopreservation is a major change in artificial insemination. It not only solves the problem of long-term storage of semen, but also benefits the protection of local pig breed resources. It also makes semen not limited by time and region, and facilitates inter-provincial and international cooperation. , improve the utilization rate of high-quality breedi...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 李青旺刘宏胡建宏江中良
Owner NORTHWEST A & F UNIV
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