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Human spermatozoa cryoprotectant

A cryoprotectant and protective agent technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of biological safety hazards, sperm function protection, biological safety hazards, etc., and achieve sperm DNA fragmentation rate The effect of lowering, good forward motion ability, and easy operation

Inactive Publication Date: 2018-08-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of commercially available cryoprotectants lies in the cryoprotective effect of sperm, especially the protective effect of sperm function is limited, and some homemade cryoprotectants contain animal-derived components such as egg yolk, which has certain biological safety hazards
Invention patent application 201301725422 discloses a cryoprotectant for human beings, which contains fresh egg yolk, which poses biological safety hazards
Invention patent application 2015110283406 discloses a yolk-free cryoprotectant, although it has a certain improvement in the freezing recovery rate, but it does not protect sperm function

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] A. After detoxification, clean the volumetric flask, measure 50 ml of five-distilled water, add 0.3 g of sodium citrate, add five-distilled water, and stir until fully dissolved;

[0018] B. Weigh 0.8 g of Tris, 0.3 g of Tris, add the above solution, and stir until fully dissolved;

[0019] C, adjust above-mentioned solution to pH7.5 with the citric acid solution of 0.1mol / L;

[0020] D. Weigh 5.5 grams of trehalose, add the above solution, and stir slowly to avoid bubbles until fully dissolved;

[0021] E. Measure 10 ml of glycerin, add the above solution, slowly to avoid bubbles, until fully dissolved;

[0022] F. Use a filter with a pore size of 0.45 micron to filter the above solution, and put the filtered solution into a sterile clean container for temporary storage;

[0023] G. Use a 0.22 micron filter to filter the above solution, take part of the filtered solution for pathogen culture, and store the rest of the solution in a freezer at -80°C. After the pathog...

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PUM

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Abstract

The invention provides a cryoprotectant suitable for cryopreservation of human spermatozoa. The cryoprotectant mainly comprises the following components: sodium citrate, trihydroxymethyl aminomethane,N-[tris(hydroxymethyl)metyl]-2-aminopropanesulfonic acid, glycerinum and mycose. The provided cryoprotectant does not have ingredients of animal origin such as yolk, and thus, potential biological safety hazards are avoided. Compared with the prior art, the cryoprotectant has the advantages that if the human spermatozoa is subjected to cryopreservation by the cryoprotectant, the recovery can be better after the human spermatozoa is unfrozen, the number of recovered forward moving spermatozoa is large, the forward moving ability is good, meanwhile, the spontaneous acrosome reaction rate and DNA fragmentation rate of the recovered spermatozoa are low, namely, the number of spermatozoa with complete acrosome is large, nuclear injury of the spermatozoa is small, and protecting ability to functions of the spermatozoa is better.

Description

technical field [0001] The invention relates to the field of cryopreservation of human cells, in particular to a human sperm cryoprotectant. Background technique [0002] The cryopreservation of human sperm is one of the prerequisites to ensure the smooth development of assisted reproductive technology. The improvement of cryopreservation technology will help to improve the quality of sperm available in clinical assisted reproduction. The perfection of sperm cryoprotectant formula is one of the key factors to improve the cryopreservation of human sperm. The formation of ice crystals during ultra-low temperature freezing damages the structure of sperm and affects the function of sperm, such as the increase of spontaneous acrosome reaction rate and DNA fragmentation rate of sperm, which in turn affects the fertilization function of sperm. Therefore, the cryoprotection of sperm not only needs to protect sperm motility, but also needs to improve the protective effect of sperm f...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 范立青周戴王行明张晗曾亚荣朱文兵
Owner CENT SOUTH UNIV
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