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Sheep seminal fluid cryopreservation diluent and method

A low-temperature preservation and diluent technology, which is applied in the field of animal husbandry, can solve the problems of short storage time of sheep semen at low temperature, and achieve the effects of easy standardization and standardization, reliable use effect, and convenient commercial production and sales

Pending Publication Date: 2019-10-25
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Claims
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Problems solved by technology

[0005] In view of this, the embodiment of the present invention provides a diluent and method for low-temperature storage of sheep semen, the main purpose of which is to solve the problems of short storage time of sheep semen at low temperature, avoiding the addition of animal-derived substances, and simplifying the operation steps of semen dilution

Method used

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Embodiment

[0018] A low-temperature preservation diluent of sheep semen, comprising the following components: 12-29g of glucose, 10-50g of sucrose, 8-13g of sodium citrate, 600,000-1,200,000 IU of penicillin, 600,000-1,200,000 IU of streptomycin, and 1,000 mL of distilled water;

[0019] The semen and diluent are mixed according to the volume ratio of 1:4 and stored in a vial, and the vial is wrapped with 4-8 layers of gauze and placed in a refrigerator at a temperature of 0-4°C.

[0020] As a preference of the above embodiment, the diluent includes the following components: glucose 26-29g, sucrose 10-26g, sodium citrate 10-13g, penicillin 80-1.2 million IU, streptomycin 80-1.2 million IU, distilled water 1000mL.

[0021] As a preference of the above embodiment, the diluent includes the following components: glucose 20-26g, sucrose 16-45g, sodium citrate 9-13g, penicillin 100-1.2 million IU, streptomycin 100-1.2 million IU, distilled water 1000mL.

[0022] As a preference of the above ...

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Abstract

The invention discloses a sheep seminal fluid cryopreservation diluent and method, and relates to the technical field of livestock breeding. The diluent comprises the following components:12-29 g of glucose, 10-50 g of sucrose, 8-13 g of sodium citrate, 600 thousand to 1.20 million IU of penicillin, 600 thousand to 1.20 million IU of streptomycin and 1000 mL of distilled water. The seminal fluid and the diluent are mixed at the ratio of 1 to 4 and stored in a small battle, and the small battle is wrapped with 4 to 8 layers of gauze and then placed in a refrigerator at 0-5 DEG C. The effectivesurvival time of the diluent is not less than 100 hours; the total survival time is not less than 140 hours; the survival index is not less than 69; the acrosome integrity rate after 48 hours of preservation is not less than 72%; the deformity rate after 48 hours of preservation is not more than 18%; substances used in the preparation of the diluent are all chemical reagents, animal derived substances such as yolk or milk are not contained, the safety and reliability are achieved, the quality is excellent, the cost is low, and the preparation method of the diluent is easy.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a diluent and method for low-temperature preservation of sheep semen. Background technique [0002] Goat sperm cannot survive for a long time in vitro, and the sperm viability and fertilization ability will decrease significantly after cryopreservation for 24 hours. In order to prolong the storage time of semen, the collected semen should be diluted with a good diluent in time. The role of the diluent is to provide new nutrients and energy for the survival of sperm; provide a buffer to prevent the harm to sperm from pH changes during the formation of lactic acid; maintain normal osmotic pressure and electrolytic balance of semen; inhibit bacterial growth; prevent rapid cooling , Low temperature causes cold shock damage to sperm; diluting semen can greatly increase the amount of semen, so as to expand the number of inseminated ewes, which is very beneficial to play the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 蔡元李春阳刘伟谭彩云冷啸仙汪喜卫马学虎李兴龙张森岚蒋家懿徐梦瑶李甜甜才让卓玛邱丽霞潘瀚琳
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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