Nutritional antifreeze diluting agent for improving sperm in-vitro storage and preparation method of nutritional antifreeze diluting agent

A diluent and nutrient technology, applied in the field of pig breeding, can solve problems such as low insemination rate, unreasonable nutrient use level and method, and decreased sperm motility, so as to achieve the goal of increasing farrowing rate, improving reproductive performance, and increasing the number of live piglets Effect

Inactive Publication Date: 2017-08-29
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current pig semen dilution formula mainly focuses on some simple ion balances. The supply of many nutrients for sperm survival, metabolism and maintenance of sperm vitality is lacking, and the level and method of some nutrients are unreasonable. Decreased vigor during transportation, lower fertilization rate, etc.

Method used

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  • Nutritional antifreeze diluting agent for improving sperm in-vitro storage and preparation method of nutritional antifreeze diluting agent
  • Nutritional antifreeze diluting agent for improving sperm in-vitro storage and preparation method of nutritional antifreeze diluting agent

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preparation example Construction

[0026] The present invention also provides a method for preparing a nutrient antifreeze diluent for improving sperm preservation in vitro, including the following steps:

[0027] Step 1. Weigh according to the following ratio: carbohydrate 5-12g, cell membrane stabilizer 4.5-6g, methionine 1-4g, leucine 1-7g, isoleucine 0.1-0.7g, valine 3- 7g, Threonine 4-8g, Tryptophan 2-6g, Histidine 2-5g, Phenylalanine 0.5-1.2g, Alanine 5-11g, L-Glutamine 2-4g, Glut Amino acid 1-5g, glycine 2-6g, L-proline 2-8g, citrulline 1-2.5g, ornithine 0.15-0.3g, spermine 0.3-0.9g, spermidine 0.1-0.3g , D-type aspartic acid 0.5-1.5g, taurine 0.5-1.5g, cysteine ​​0.1-0.5g, aminoethyl sulfinic acid 0.3-0.5g, Tris 2.5-6g, glycerol 2-6g , Citric acid 1.2-2g, Penicillin 80-10 million IU, Disodium edetate 0.15-0.4g, the balance is double distilled water, the total volume of the above is 1000mL;

[0028] Step 2. Put the weighed carbohydrates, methionine, leucine, isoleucine, valine, threonine, tryptophan, histid...

Embodiment 1

[0035] Combine 3g fructose, 1.5g glucose, 1.5g lactose, 1.5g methionine, 2g leucine, 0.1g isoleucine, 3.5g valine, 4g threonine, 2g tryptophan, and histamine Acid 2g, Phenylalanine 0.5g, Alanine 5g, L-Glutamine 2g, Glutamic Acid 2g, Glycine 2g, L-Proline 2g, Citrulline 2g, Ornithine 0.2g , Spermine 0.3g, spermidine 0.1g, D-aspartic acid 0.5g, taurine 0.5g, cysteine ​​0.1g, aminoethyl sulfinic acid 0.3g, Tris 3g, add 500ml double Distilled water is used to make diluent base liquid and stir evenly. Then add 1g of soybean lecithin, 2g of egg yolk lecithin, 2g of glycerin, 1.5g of citric acid, 800,000 IU of penicillin, and 0.2g of disodium edetate, stir well, add double distilled water to 1000mL, adjust the pH It is 6.9-7.2, and the osmotic pressure is 290mOsM. It can be stored at 16-18℃.

Embodiment 2

[0037] Mix 2g fructose, 2g glucose, 1g lactose, methionine 2g, leucine 2.5g, isoleucine 0.15g, valine 3g, threonine 5g, tryptophan 3g, histidine 4g, phenylalanine It is 0.6g, alanine 5g, L-glutamine 4g, glutamic acid 2g, glycine 4g, L-proline 3g, citrulline 2.5g, ornithine 0.3g, spermine 0.5g, sub Spermine 0.15g, D-Aspartic acid 0.8g, Taurine 0.8g, Cysteine ​​0.15g, Aminoethyl sulfinic acid 0.4g, Tris 5g, add 500ml double distilled water to make the diluent base Liquid, stir evenly. Then add 2g of soy lecithin, 3g of egg yolk lecithin, 3g of glycerin, 2g of citric acid, 1 million IU of penicillin, 0.3g of disodium edetate, stir well, add double distilled water to 1000mL, adjust the pH to 6.9-7.2, the osmotic pressure is 300mOsM. Store at 16-18°C.

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Abstract

The invention discloses a nutritional antifreeze diluting agent for improving sperm in-vitro storage and a preparation method of the nutritional antifreeze diluting agent. The nutritional antifreeze diluting agent is prepared from carbohydrates, a cytomembrane stabilizing agent, amino acids, Tris, glycerin, citric acid, penicillin, disodium edetate and double distilled water, wherein the pH is 6.9-7.2, and the osmotic pressure is 300mOsM. Sperm motility, acrosome integrity and plasmolemma permeability are all improved to different extents while the sperm malformation rate is decreased, and remarkable effects are achieved. By application of the nutritional antifreeze diluting agent to boar semen, farrowing rate and live farrowing quantity of sows can be increased, and reproductive performances of the sows are improved.

Description

Technical field [0001] The invention belongs to the technical field of pig reproduction, and specifically relates to a nutritional antifreeze diluent for improving sperm preservation in vitro and a preparation method thereof. Background technique [0002] In recent years, artificial insemination technology has the advantages of increasing the utilization rate of boars and increasing the conception rate of sows; overcoming the physical differences between males and females; preventing the spread of infectious diseases and being free from time and space constraints. More and more are used in animal production. In pig breeding, the rate of artificial insemination has exceeded 90%. It is precisely because of the rapid development of this technology that people pay great attention to the quality of boar semen, especially the semen quality after transportation and preservation after dilution. The quality of semen directly affects the conception rate and litter size of sows during estr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 林燕吴德徐盛玉张军杰车炼强方正锋冯斌李健
Owner SICHUAN AGRI UNIV
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