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Frozen semen diluent and preparation method thereof

A technology of freezing semen and diluent, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of low vitality, low fertilization rate, and artificial insemination, and achieve the effect of improving quality and vitality

Active Publication Date: 2009-10-14
NORTHWEST A & F UNIV
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AI Technical Summary

Problems solved by technology

However, in production practice, there are often cases of low vitality and low fertilization rate after freezing and thawing sperm, which affects the effect of artificial insemination

Method used

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Embodiment Construction

[0038] According to the technical scheme of the present invention, the substances needed to prepare 100 ml of frozen semen diluent are: sodium citrate: 0.9-1.2 grams, sucrose: 3-5 grams, egg yolk: 18-22 milliliters, glycerol: 4-7 milliliters, Vitamin E: 6-9 mg, vitamin C: 500-700 mg, spectinomycin: 35 mg, gentamicin: 80,000 units (IU).

[0039] Its best formula for frozen semen dilution is: the content of substances per 100ml is: sodium citrate: 1.073mg, sucrose: 4.344mg, egg yolk: 20ml, glycerin: 6ml, vitamin E: 8mg, vitamin C: 600 mg, spectinomycin: 35 mg, 80,000 units (IU) of gentamicin.

[0040] Its preparation method is:

[0041] 1) Weigh the raw materials in the formula: sodium citrate, sucrose, egg yolk, glycerin, vitamin E, vitamin C, distilled water.

[0042] 2) Prepare the above sodium citrate, sucrose, egg yolk, vitamin C, vitamin E, glycerin, and distilled water to form a base solution, stir with a magnetic stirrer for 60 minutes to obtain a mixed solution, and s...

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Abstract

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

Description

technical field [0001] The invention belongs to the technical category of improving bovine artificial insemination, and in particular relates to a frozen semen diluent used for bovine frozen semen to improve the quality of bovine sperm after freezing and a preparation method thereof. Background technique [0002] The successful freezing of domestic animal semen has had a profound impact on the development of artificial insemination technology. Firstly, it has laid the foundation for expanding the utilization rate and prolonging the utilization time of excellent breeding males. Secondly, it has provided a broad scope for the development of artificial insemination and low temperature biology space. Because frozen semen is more convenient in transportation, no matter in terms of time or space, the mating efficiency of male animals can be fully exerted, and the pace of livestock breed improvement has been accelerated. Although all countries have launched research on frozen seme...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 昝林森赵宪林刘永峰胡建宏田万强孙永刚桂林生
Owner NORTHWEST A & F UNIV
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