Diluent for preserving rabbit sperms at normal temperature and preparation method thereof

A technology of preservation at room temperature and diluent, which is applied in the field of animal reproduction, can solve the problems that affect the popularization and application of artificial insemination technology of rabbits, cannot be popularized and applied in rural areas and pastoral areas, and the price of imported products is expensive, so as to achieve simple production, convenient use and storage , cost reduction effect
CN102318596AInactive Publication Date: 2012-01-18青岛康大食品有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
青岛康大食品有限公司
Publication Date
2012-01-18
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to a diluent for preserving rabbit sperms at normal temperature and a preparation method thereof and belongs to the technical field of animal reproduction. The diluent is prepared by adding water into 30-40g of tris(hydroxymethyl)aminomethane, 15-20g of citric acid, 6-15g of dextrose, 0.5-1g of potassium chloride, 0.2-0.5g of glutathione and 0.05-0.1g of kanamycin until the total volume reaches 1L; and the pH value of the diluent is 6.8-7.0. By using the diluent, the rabbit sperms can be preserved for a short time, and the sperm viability can be 0.7 and the acrosome integrity rate can be 87% after the rabbit sperms are preserved by using the diluent for 48 hours at normal temperature, thus the sperm viability and the acrosome integrity rate exceed the requirements of artificial insemination of rabbits, the conception rate is up to 89.4%, and the conception rate reaches or is superior to those obtained by using the similar import diluents. Meanwhile, the diluent provided by the invention is used and stored more conveniently, and the diluent can be instant availably at the time of artificial insemination, thus being convenient and quick. The diluent is prepared simply and can be used for lowering the cost of the artificial insemination of the rabbits.
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Description

Technical field

[0001] The invention relates to the technical field of animal reproduction, in particular to a diluent for preservation of rabbit semen at room temperature, which is used for artificial fertilization of rabbits. Background technique

[0002] my country is the world's largest country for raising rabbits, but the efficiency of rabbit breeding in our country is only about 50% of that of developed countries in Europe. The low breeding efficiency restricts the development of the rabbit industry in China. The insufficient application and promotion of artificial insemination technology is an important factor leading to the low efficiency of the rabbit industry in my country. The preservation of semen is a bottleneck that affects the application and promotion of artificial insemination technology in rabbits. The artificial insemination technology of rabbits has the advantages of saving breeding costs, improving labor efficiency, reducing labor intensity, increasing concep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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