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Preserving liquid for swine sperm
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A technology for preserving liquid and porcine semen, which is applied in the field of semen preservation liquid and porcine semen preservation liquid, and can solve the problems of reducing sperm metabolism, semen waste, expensive price, etc.
Active Publication Date: 2010-06-16
HANGZHOU DONGYUAN BIOLOGICAL TECH
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Problems solved by technology
[0005] However, under the temperature condition of room temperature storage (15-25°C), it is difficult to reduce the metabolism of sperm and inhibit the growth of microorganisms. The dilution of pig semen and the quality of the preservation solution directly affect the survival time of sperm
The existing pig semen preservation solution at room temperature has a short storage time, which leads to a large amount of waste of semen; at the same time, the imported pig semen preservation solution is expensive and cannot meet the needs of China's vast rural and pastoral areas
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Embodiment 1
[0013] Embodiment 1: a kind of preservation solution of pig semen, its component distribution ratio (by weight) is: anhydrous glucose 25.5g / l, trisodium citrate dihydrate 5.1g / l, sodiumbicarbonate 2g / l, EDTA 2g / l, potassiumchloride 1g / l, citric acid 2.4g / l, TRIS 8.5g / l, AMK 0.26g / l, V-C 0.2g / l.
[0016] (2) Put the components of step (1) into the container, add distilled water to dissolve to 1 liter, and shake well;
[0017] (3) Filter the solution obtained in step (2) to obtain a preservation solution, and adjust the pH value to 7.2-7.4;
[0018] (4) When used, it is used at a ratio of 1 part of semen to 2 parts of preservation solution, and stored at a temperature of 16-22°C.
Embodiment 2
[0019] Embodiment 2: a kind of preservation solution of pig semen, its component distribution ratio (by weight) is: anhydrous glucose 35.0g / l, trisodium citrate dihydrate 7.0g / l, sodium bicarbonate 10g / l, EDTA 4g / l, potassium chloride 3g / l, citric acid 3.4g / l, TRIS 9.5g / l, AMK 1g / l, V-C 1g / l.
[0020] The preparation method is the same as in Example 1.
Embodiment 3
[0021] Embodiment 3: a kind of preservation solution of pig semen, its component distribution ratio (by weight) is: anhydrous glucose 30.0g / l, trisodium citrate dihydrate 6.0g / l, sodium bicarbonate 5.5g / l, EDTA 3g / l, potassium chloride 2g / l, citric acid 3.0g / l, TRIS 9.0g / l, AMK 0.6g / l, V-C 0.6g / l.
[0022] The preparation method is the same as in Example 1.
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Abstract
The invention relates to preserving liquid for sperm, in particular to the preserving liquid for swine sperm, and belongs to the technical field of animal reproduction. The preserving liquid for the swine sperm comprises the following components: 25.5 to 35.0g / l of anhydrous dextrose, 5.1 to 7.0 g / l of trisodium citrate, 1 to 10 g / l of sodiumbicarbonate, 2 to 4g / l of EDTA, 1 to 3g / l of potassiumchloride, 2.4 to 3.4g / l of citric acid, 8.5 to 9.5g / l of trihydroxymethylaminomethane, 0.26 to 1g / l of amikacin and 0.2 to 1g / l of vitamin C. Through detection, in the swine sperm preserved by the preserving liquid, the sperm viability reaches 0.60 to 0.85 and the integrity rate of sperm acrosome reaches 90 percent, which excess the requirement of the current artificial fertilization.
Description
technical field [0001] The invention relates to a semen preservation solution, in particular to a porcine semen preservation solution, belonging to the technical field of animal reproduction. technical background [0002] Artificial insemination can be said to be the most important management work to improve the reproductive rate of livestock and poultry. Artificial insemination has been used on a large scale in horse, poultry, cattle and sheep production since the early 20th century when Danish stallion breeders Sand and Stribolt used it as a commercial tool to maximize the use of the semen of elite stallions . [0003] Artificial insemination technology has the following advantages: 1. Use genetically superior breeding stock (poultry) semen to inseminate numerous female animals (poultry), which significantly increases the speed of genetic improvement; 2. Controls the spread of certain pathogens to Improve the health of livestock and poultry, improve the biosecurity level...
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