The invention belongs to the field of feed additives and particularly discloses an intestinal tract health protection agent capable of reducing stress of weanling pigs as well as a preparation method and a use method thereof. The intestinal tract health protection agent consists of the following ingredients: plant essential oil, radix astragali polysaccharose, licorice root extracts, honeysuckle flower extracts, lactobacillus, bacillus bifidus, fructooligosaccharide, tryptophan, glutamine, arginine, ferrous fumarate, zinc lactate, selenium yeast, vitamin C, vitamin B12, folic acid, vitamin B6, citric acid, lactic acid, phosphoric acid, nucleotide, taurine, acid proteinase, alkali protease, alpha-amylase and anhydrous glucose. The intestinal tract health protection agent disclosed by the invention is capable of effectively relieving weanling stress of piglets, and preventing stress disease (common diarrhoea) caused by the stress factor.