Swine semen freezing protecting agent and cryopreserving method for swine semen by using same

A technology of cryoprotectant and porcine semen, which is applied in the field of porcine semen preservation, can solve the problems of conception rate, low litter size, difficulty in meeting production needs, etc., so as to improve the survival rate of porcine sperm, ensure fertilization vitality and use effect, Improve the effect of cryopreservation

Inactive Publication Date: 2018-08-24
TONGLING XINMENGXIANG AGRI & ANIMAL HUSBANDRY TECH CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic and foreign researchers have done a lot of research on the cryopreservation of pig sperm, the conception rate and litter size of pig sperm after freezing and thawing are still far below the effect of normal temperature liquid semen, which is difficult to meet the actual production needs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A pig semen cryoprotectant of the present invention is composed of the following components in parts by weight: 2.5 parts of glucose, 2 parts of citric acid, 3 parts of coconut oil monoglycolyl, 2.5 parts of hyaluronic acid, 1.5 parts of skim milk, trehalose 1.2 parts, 0.07 parts of penicillin sodium, 0.2 parts of streptomycin sulfate, 95 parts of double distilled water, 23 parts of fresh egg yolk, 3.5 parts of glycerin, 1.5 parts of astaxanthin and 5 parts of Rehmannia glutinosa extract.

[0017] The above-mentioned pig semen cryoprotectant is used for the cryopreservation method of pig semen, comprises the steps:

[0018] 1) Manually collect fresh pig semen and add it to a centrifuge tube;

[0019] 2) Place the centrifuge tube in step 1) in warm water at 33°C for preheating, and the preheating time is 40 minutes;

[0020] 3) Put the preheated centrifuge tube containing pig semen into a centrifuge for centrifugation, then filter the centrifuged pig semen through ten l...

Embodiment 2

[0025] A pig semen cryoprotectant of the present invention is composed of the following components in parts by weight: 2 parts of glucose, 1.5 parts of citric acid, 2.5 parts of coconut oil monoglycolyl, 2 parts of hyaluronic acid, 1 part of skim milk, trehalose 1 part, 0.05 parts of penicillin sodium, 0.1 part of streptomycin sulfate, 90 parts of double distilled water, 20 parts of fresh egg yolk, 3 parts of glycerin, 1 part of astaxanthin and 4 parts of Rehmannia glutinosa extract.

[0026] The above-mentioned pig semen cryoprotectant is used for the cryopreservation method of pig semen, comprises the steps:

[0027] 1) Manually collect fresh pig semen and add it to a centrifuge tube;

[0028] 2) Place the centrifuge tube in step 1) in warm water at 30°C for preheating, and the preheating time is 35 minutes;

[0029] 3) Put the preheated centrifuge tube containing pig semen into a centrifuge for centrifugation, then filter the centrifuged pig semen through ten layers of ste...

Embodiment 3

[0034] A pig semen cryoprotectant of the present invention is composed of the following components in parts by weight: 3 parts of glucose, 2.5 parts of citric acid, 3.5 parts of coconut oil monoglycolyl, 3 parts of hyaluronic acid, 2 parts of skim milk, trehalose 1.5 parts, 0.08 parts of penicillin sodium, 0.3 parts of streptomycin sulfate, 100 parts of double distilled water, 25 parts of fresh egg yolk, 4 parts of glycerin, 2 parts of astaxanthin and 6 parts of Rehmannia glutinosa extract.

[0035] The above-mentioned pig semen cryoprotectant is used for the cryopreservation method of pig semen, comprises the steps:

[0036] 1) Manually collect fresh pig semen and add it to a centrifuge tube;

[0037] 2) Place the centrifuge tube in step 1) in warm water at 35°C for preheating, and the preheating time is 45 minutes;

[0038] 3) Put the preheated centrifuge tube containing pig semen into a centrifuge for centrifugation, then filter the centrifuged pig semen through ten layers...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a swine semen freezing protecting agent and a cryopreserving method for swine semen by using the same. The swine semen freezing protecting agent is prepared from the followingcomponents in parts by weight: 2.5 parts of glucose, 2 parts of citric acid, 3 parts of coconut oil monoethanolamide, 2.5 parts of hyaluronic acid, 1.5 parts of skimmed milk, 1.2 parts of trehalose,0.07 part of penicillin sodium, 0.2 part of streptomycin sulfate, 95 parts of double distilled water, 23 parts of fresh yolk, 3.5 parts of glycerin, 1.5 parts of astaxanthin and 5 parts of extract ofradix rehmanniae praeparata. The cryopreserving method comprises the following steps of firstly, collecting the swine semen, centrifuging, and filtering, so as to obtain the swine semen A; pouring onepart of swine semen A into an experiment bottle, and adding two parts of freezing protecting agent to thin; finally, adding into liquid nitrogen, and cryopreserving. The swine semen freezing protecting agent has the advantages that the freezing protecting agent is reasonably compounded, so that the cryopreserving effect of the swine semen is improved under the function of the components; the survival rate, acrosome integrity and plasmalemma integrity of the swine semen are improved; the insemination activity and use effect of the swine semen are guaranteed.

Description

technical field [0001] The invention relates to the technical field of porcine semen preservation, in particular to a cryoprotectant for porcine semen and a cryopreservation method for porcine semen. Background technique [0002] In my country, pigs are the most important livestock, and pork production accounts for about 70% of the total meat in the country. In recent years, with the improvement of people's living standards, the direction of pig breeding has changed from mainly focusing on the improvement of growth rate and lean meat percentage to improving the quality of pork and the flavor of meat. The traditional breeding method has a long generation interval and cannot meet the market demand. The use of frozen pig semen technology can make full use of high-quality breeding stock, shorten the generation interval of fine-bred breeding, and quickly establish new breeding pigs and commercial pig hybrids that meet market requirements. breeding system. [0003] With the prom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 黄义灯
Owner TONGLING XINMENGXIANG AGRI & ANIMAL HUSBANDRY TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap