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Antifreezer for diluting boar semen

A technology of antifreeze and pig semen, which is applied in the field of animal reproduction, can solve problems such as DNA damage, denaturation, and DNA strand breakage, and achieve the effects of enhancing protection, inhibiting damage rate, and improving quality

Inactive Publication Date: 2009-03-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fraser et al. (Folia Histochem Cytobiol.2004) confirmed that the freezing and thawing process of pig sperm can easily cause DNA damage. It can be seen that cryopreservation may also cause DNA strand breakage and denaturation during the freezing-thawing recovery process of pig sperm.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] First weigh 0.05g of N-acetyl-D-glucosamine, dissolve it in 5ml of distilled water, and dissolve it completely.

[0016] Then weigh 3.70g of glucose and dissolve it in 20ml of distilled water. After completely dissolving, add 0.24g of tris, 0.60g of sodium citrate, 0.12g of sodium bicarbonate, 0.07g of potassium chloride, and 0.4mM of hyaluronic acid. , add one by one in the above order, the reagent added first is completely dissolved, and then the latter one is added to make it completely dissolved.

[0017] Mix the two preparations, add 0.5ml of amino-sodium-dodecyl sulfate, 20ml of egg yolk and 3ml of glycerin, then add 0.06g of penicillin sodium and 0.10g of streptomycin sulfate, and finally distill water to 100ml. The final pH value was 7.2, the osmotic pressure was 320mOsmo / L, and it was stored in a refrigerator at 5°C. All preparation processes are carried out under sterile conditions.

[0018] This formula has an excellent effect on the cryopreservation of por...

Embodiment 2

[0021] First weigh 0.10g of N-acetyl-D-glucosamine, dissolve it in 5ml of distilled water, and dissolve it completely.

[0022] Then weigh 3.98g of glucose and dissolve it in 20ml of distilled water. After completely dissolving, add 0.46g of tris, 0.58g of sodium citrate, 0.14g of sodium bicarbonate, 0.08g of potassium chloride, and 0.8mM of hyaluronic acid. , add one by one in the above order, the first added reagent is completely dissolved, and then the latter one is added to make it dissolve.

[0023] Then mix the two preparations, add 1.2ml of amino-sodium-lauryl sulfate, 22ml of egg yolk and 4ml of glycerin, then add 0.08g of penicillin sodium and 0.15g of streptomycin sulfate, and finally distilled water to 100ml. After storage, the pH value was 7.4, the osmotic pressure was 345mOsmo / L, and it was stored in a refrigerator at 5°C. All preparation processes are carried out under sterile conditions.

[0024] This formula has an excellent effect on the cryopreservation of ...

Embodiment 3

[0027] First weigh 0.02g of N-acetyl-D-glucosamine, dissolve it in 5ml of distilled water, and dissolve it completely.

[0028] Then weigh 3.0g of glucose and dissolve it in 20ml of distilled water. After completely dissolving, add 0.20g of tris, 0.5g of sodium citrate, 0.10g of sodium bicarbonate, 0.05g of potassium chloride, and 0.2mM of hyaluronic acid. , add one by one in the above order, the first added reagent is completely dissolved, and then the latter one is added to make it dissolve.

[0029] Then mix the two preparations, add 0.2ml amino-sodium-lauryl sulfate, 18ml egg yolk and 2ml glycerin, then add 0.05 gram penicillin sodium and 0.08 gram streptomycin sulfate, and finally distilled water to 100ml, After storage, the pH value is 6.8, the osmotic pressure is 310mOsmo / L, and it is stored in a refrigerator at 5°C. All preparation processes are carried out under sterile conditions.

[0030] This formula has an excellent effect on the cryopreservation of porcine seme...

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Abstract

The invention relates to an antifreeze agent for diluting pig sperm, pertaining to the technical field of animal breeding. The antifreeze agent is prepared by N-acetyl-D-dextrosamine, hyaluronic acid, amido-sodium-lauryl sulfuric ester, glucose, trihydroxymethyl aminomethane, sodium citrate, sodium bicarbonate, potassium chloride, bactericide, distilled water and the like, wherein, the N-acetyl-D-dextrosamine can stimulate the emulsification function of the amido-sodium-lauryl sulfuric ester, enhances the protection function of the amido-sodium-lauryl sulfuric ester and yolk, the hyaluronic acid and the amido-sodium-lauryl sulfuric ester can effectively inhibit the damage rate of freezing and thawing sperm plasma membrane and DNA and improve the quality of freezing and thawing sperm, the glucose is the energy source for metabolism in vitro of the sperm, and the sodium citrate can maintain the osmotic pressure balance between diluted antifreeze agent environment and the internal environment of the sperm. The antifreeze agent for diluting pig sperm can effectively prolong the survival time of pig sperms for ultra-low temperature cryopreservation, and improve the liveliness of the freezing and thawing pig sperms, the integrity of plasma membrane and the integrity of acrosome.

Description

technical field [0001] The invention relates to a porcine semen dilution antifreeze agent, which is used for inhibiting the damage to sperm DNA during the freezing recovery process and prolonging the survival time of porcine sperm cryopreservation at ultra-low temperature. The invention belongs to the technical field of animal reproduction. Background technique [0002] Sweden, the United Kingdom, Canada and other countries have established artificial insemination technology platforms and industrialized them in livestock animal breeding and actual reproduction production, with remarkable economic benefits. my country is a big pig raising and consumption country. At the end of 2007, the number of pigs on hand was about 550 million, and the pork output was about 55 million tons. With the improvement of people's living standards, the per capita consumption of pork has increased year by year, and the scale of breeding has continued to increase. The way of raising pigs has gradua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A01N1/02
Inventor 李新红王亮亮
Owner SHANGHAI JIAO TONG UNIV
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