Composition and method to increase mammalian sperm function

A composition and technology of sperm, applied in the direction of biochemical equipment and methods, drug combination, preservation of human or animal body, etc., can solve the problem that the biological role has not been determined

Inactive Publication Date: 2009-04-01
TMI LAB INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Members of the DNase I-like family differ from DNase I in terms of enzymatic activity, regulation of expression, and location, and their biological roles have not been determined

Method used

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  • Composition and method to increase mammalian sperm function
  • Composition and method to increase mammalian sperm function
  • Composition and method to increase mammalian sperm function

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Embodiment

[0078] The following examples are included merely to provide a more complete disclosure of the invention described and claimed herein. The examples do not limit the scope of the invention in any way.

[0079] Chemicals and reagents were obtained commercially from Sigma Chemical (St. Louis, Missouri) unless otherwise noted.

[0080] 1. Cloning and Sequence Analysis

[0081] (a) Tissue inhibitor of metalloproteinase type 2 (TIMP-2):

[0082] Purification of the 24 kDa seminal heparin-binding protein (HBP-24) was previously reported (McCauley et al., 2001). Microsequence analysis of semen-purified HBP-24 identified 20 N-terminal amino acid residues that are compatible with bovine metalloproteinase inhibitors identified as tissue inhibitors of metalloproteinase-2 (TIMP-2; De Clerck et al., 1989) The N-terminus has 90% identity. To clone the semen TIMP-2 gene, bovine TIMP-2 gene-specific primers were designed based on the published bovine aorta cDNA sequence (Boone et al., 1990...

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Abstract

A composition of matter for increasing the motility and/or percentage of intact acrosomes (PIA) in sperm is described. The composition includes, in combination, an amount of FAA and an amount of TIMP-2, wherein the amounts are effective to increase the motility, the PIA, or the motility and PIA of sperm contacted with the composition. The composition can be used as a cryopreservation medium for sperm.

Description

[0001] Cross References to Related Applications [0002] Priority is hereby claimed to Provisional Application Serial No. 60 / 760,312 filed January 19, 2006, which is hereby incorporated by reference. field of invention [0003] The present invention relates to a composition comprising a combination of a fertility associated antigen (FAA) and a tissue inhibitor of metalloproteinase type 2 (TIMP-2). The present invention also relates to methods of using the compositions for enhancing the functionality of mammalian spermatozoa in general, and more specifically spermatozoa contained in cryopreserved semen. [0004] bibliography [0005] Full bibliographic citations for papers mentioned herein are included in the bibliography section immediately following the claims. Papers mentioned in the bibliography are hereby incorporated by reference. Background of the invention [0006] It has been well documented that semen is a complex mixture of secretions from the male accessory rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/81C07K14/47C12N5/06C12N5/076
CPCC12N5/061A01N1/02C07K14/8146A01N1/0226A61P15/08A61P43/00
Inventor T·C·麦考利H·张R·L·阿克斯
Owner TMI LAB INT
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