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Three-dimensional scaffold material for bone tissue repair and preparation method thereof

A three-dimensional scaffold and bone tissue technology, used in medical science, prosthesis, etc., can solve the problems of limited application, poor mechanical properties of microporous scaffolds, easy deformation, etc., achieve excellent mechanical properties, be conducive to repair and regeneration, and promote regeneration. Effect

Inactive Publication Date: 2014-08-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of this kind of microporous scaffold are poor, especially in wet state, which limits its application in bone tissue engineering.

Method used

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  • Three-dimensional scaffold material for bone tissue repair and preparation method thereof
  • Three-dimensional scaffold material for bone tissue repair and preparation method thereof
  • Three-dimensional scaffold material for bone tissue repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (a) Polycaprolactone (PCL, molecular weight 65,000) is heated to 60 degrees Celsius and extruded from a needle with an inner diameter of 0.4 mm to form a 0.4 mm PCL fluid. The moving speed of the receiving plate is 0.05 mm / s, and the polymer extrusion rate 0.05 mm / s, the fluid spacing is 0.5 mm, the layers are vertically arranged, the layer height is 0.3 mm, and the number of layers is 7 layers, forming a cylindrical PCL skeleton with a diameter of 20 mm ( figure 2 , image 3 ).

[0039] (b) 1 gram of mulberry silk regenerated silk fibroin solid was dissolved in 50 milliliters of deionized water, and stirred to completely dissolve it to obtain a silk fibroin solution with a mass volume percentage of 2%.

[0040] (c) Placing the PCL skeleton in a plastic mold, and injecting the prepared silk fibroin solution to completely infiltrate and submerge the PCL skeleton. The mold was frozen in a refrigerator at -80 degrees Celsius for 12 hours, and then freeze-dried in a free...

Embodiment 2

[0044] (a) PCL is heated to 60 °C and extruded by a needle with an inner diameter of 0.4 mm to form a 0.4 mm PCL fluid, the receiving plate moving speed is 0.05 mm / s, the polymer extrusion rate is 0.05 mm / s, and the fluid spacing is 0.5 mm, the layers are arranged vertically between the layers, the layer height is 0.3 mm, and the number of layers is 7 layers to obtain a cylindrical PCL skeleton with a diameter of 14 mm.

[0045] (b) Dissolve 1 gram of gelatin (type A pigskin gelatin) and 1 gram of mulberry silk regenerated silk fibroin in 50 ml of deionized water, heat to 60 degrees Celsius and stir to completely dissolve the gelatin and silk fibroin to obtain a mass volume percentage of 4%. gelatin / silk fibroin (1:1) aqueous solution.

[0046] (c) Placing the PCL skeleton in a plastic mold, and injecting the prepared silk fibroin solution to completely infiltrate and submerge the PCL skeleton. The mold was frozen in a -80°C refrigerator for 12 hours, and then freeze-dried in...

Embodiment 3

[0049] (a) Add 3 grams of PCL into 30 milliliters of trifluoroethanol, stir to dissolve, add 1.5 grams of nano-hydroxyapatite particles after the dissolution is complete, continue to stir and ultrasonically disperse the hydroxyapatite evenly. The prepared PCL / nano-hydroxyapatite solution was poured into a metal container, and the trifluoroethanol was quickly volatilized and completely removed by heating to obtain a mixture of PCL and nano-hydroxyapatite with a mass ratio of 2 / 1. This mixture was heated to 60°C and extruded with a needle with an inner diameter of 0.5 mm to form a 0.5 mm PCL fluid with a receiving plate moving speed of 0.05 mm / s, a polymer extrusion rate of 0.05 mm / s, and a fluid spacing of 0.5 mm , the layers are arranged at 60 degrees, the layer height is 0.4 mm, and the number of layers is 9 layers to obtain a cylindrical PCL skeleton with a diameter of 20 mm.

[0050] (b) Add 0.4 g of collagen (type I pigskin collagen) into 20 ml of deionized water, stir to ...

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Abstract

The invention relates to a three-dimensional scaffold material for bone tissue repair and a preparation method thereof. The three-dimensional scaffold material consists of a three-dimensional printing polymer framework with an interpenetrating macroporous structure and a protein material which serves as a filling material and has a microporous structure. The preparation method comprises the following steps: 1) preparing a polymer three-dimensional framework by a three-dimensional printer; 2) preparing an aqueous solution of protein; 3) carrying out freeze drying; and 4) adding a crosslinking agent, washing with water and then carrying out freeze drying again to obtain the product. The three-dimensional scaffold material provided by the invention is simple to operate, high in stability, free from organic solvents in the whole course and green and safe. The prepared bone tissue scaffold is good in mechanical property. The polymer in the scaffold is tightly combined with proteins. The polymer scaffold can provide an enough brute force while the protein part can provide more cell adhesion sites to benefit penetrative growth of cells, thereby promoting tissue regeneration.

Description

technical field [0001] The invention belongs to the field of tissue engineering and biomanufacturing, in particular to a three-dimensional scaffold material for bone tissue repair and a preparation method thereof. Background technique [0002] At present, the incidence of bone defects due to trauma, tumor resection, infection, abnormal development, etc. is high, which seriously affects the daily life of patients. Existing methods are mainly autologous bone and allogeneic bone transplantation. There are few sources of autologous bone, and it will bring some pain to the patient; allogeneic bone transplantation is likely to cause rejection, and its application is limited. In recent years, the development of bone tissue engineering based on biomaterials provides a new way to treat bone defects. Bone tissue engineering includes three elements: seed cells, growth factors and scaffolds. Among them, the three-dimensional space scaffold can provide adhesion sites, nutrient delivery...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/56A61L27/54
Inventor 莫秀梅李大伟许杜亮柯勤飞
Owner DONGHUA UNIV
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