Injectable composite material for bone repair and preparation method thereof

A composite material and bone repair technology, applied in the fields of material science, orthopedics and plastic surgery, can solve the problems of complex extraction process, single component, immune and inflammatory response, etc., achieve excellent biocompatibility, enhance biological activity, induce bone effect of growth

Active Publication Date: 2016-02-10
HANGZHOU HUAMAI MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this type of collagen is that the extraction process is complicated, the protein is denatured during the extraction process, and cannot exert its best biological performance; in addition, its composition is relatively single, which cannot meet the complex signaling pathway requirements in the bone repair process; the use process It needs to use highly toxic chemical cross-linking agents, which can easily cause immune and inflammatory reactions during the implantation process.

Method used

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  • Injectable composite material for bone repair and preparation method thereof
  • Injectable composite material for bone repair and preparation method thereof
  • Injectable composite material for bone repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Preparation and Characterization of Biological Tissue Matrix / Calcium Hydrogen Phosphate Composite Material

[0060] 1) Take the microfibrous biological tissue matrix material after Co60 gamma ray-sterilized pig skin decellularization and antigen removal, and suspend it in 0.9% physiological saline at a concentration of 10 mg / ml;

[0061] 2) Wash more than three times with sterile deionized water, the parameters of the prepared acellular matrix microfibers are as follows: figure 1 As shown, its average aspect ratio is 0.05-0.95, and its particle size distribution is 40-1000 microns;

[0062] 3) Take calcium hydrogen phosphate microparticles, add sterile deionized water, and prepare a uniform suspension of 10 mg / ml

[0063] 4) Dibasic calcium phosphate microparticles are rapidly passed through a filter membrane with a particle diameter of 40 microns to remove excessively large agglomerated particles;

[0064] 5) Centrifuge the suspension at 1200 rpm for 2 min...

Embodiment 2

[0072] Example 2: Bone defect repair experiment of biological tissue matrix / calcium hydrogen phosphate composite injectable material

[0073] The injectable biological tissue matrix / calcium hydrogen phosphate composite material prepared by the invention is used to evaluate the bone defect repairing ability of the material in the skull defect model of athymic nude mice. After the mouse (6 examples) was anesthetized, the head fur was shaved off, and a 1 cm opening was opened on the top of the head. After the skin was pulled away, a prototype defect with a diameter of 3.5 mm was punched on the skull with a dental drill; the material prepared by the present invention After the bone defect was filled, the wound was sutured; six weeks later, the mice were humanely sacrificed, and the repaired skull was observed with the naked eye, and the material and the surrounding bone tissue were well compounded, and X-ray observations showed that a large amount of bone tissue was formed in the d...

Embodiment 3

[0074] Example 3: Bone defect repair experiment of biological tissue matrix / calcium hydrogen phosphate composite injectable material as fresh bone marrow carrier

[0075] The injectable biological tissue matrix / calcium hydrogen phosphate composite material prepared in Example 1 was mixed with freshly extracted mouse whole bone marrow to prepare a pasty material; using the mouse model used in Example 2, the mixed mouse whole bone marrow The hybrid composite material was used to fill the bone defect, and the wound was then sutured; six weeks later, X-ray observations revealed that the entire prototype bone defect had been filled with newly formed bone tissue, and no tissue gaps were found at the edge of the bone defect. Micro-CT scan atlases showed the same result, the entire bone defect had been filled with newly formed bone tissue, and a seamless connection was formed between the new bone and the autogenous bone. Quantitative analysis of bone formation shows that the material ...

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Abstract

The invention relates to an injectable composite material for bone repair. According to the composite material, biological tissue materials and biological ceramics are organically combined so as to form an injectable medical material with a three-dimensional scaffold function. In the invention, a biological tissue material substrate is micro-fiber with a natural crosslinking structure, is free of additional physical or chemical crosslinking, has good biocompatibility, and can be slowly degraded completely in vivo; and the biological tissue materials and the biological ceramics can be combined in multiple ways. In the composite material, after the biological ceramics serving as a reinforced phase are combined with the biological tissue materials, a favorable template can be provided for bone tissue regeneration in vivo, and bone growth can be effectively induced. The injectable material provided by the invention can be used for seamlessly filling bone defect of any size, and biological agents including bone marrow are added in the process, so that the bioactivity is further enhanced. Therefore, the material can be widely used for bone defect caused by wounds, resection of tumors, osteonecrosis, infection and the like.

Description

technical field [0001] The invention belongs to the field of material science, and in particular relates to an injectable composite material for bone repair and a preparation method thereof. The composite material can be widely used in the fields of orthopedics and orthopedics. Background technique [0002] The repair and reconstruction of damaged bone tissue is an important task for orthopedic surgeons all over the world, and the repair of damaged bone tissue requires the use of bone graft materials. The gold standard used worldwide for repairing damaged bone tissue is human autologous bone tissue, ie healthy bone tissue taken from the patient's own body. Autologous bone has all the properties needed to repair bone tissue, such as excellent osteoinductivity, osteoconductivity, osteogenesis and safety. However, the use of autologous bone has unavoidable drawbacks clinically. Since the use of autologous bone requires obtaining bone tissue from a donor site in the patient's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/46A61L27/50
CPCA61L27/46A61L2430/40A61L27/3834A61L27/54A61L2300/64A61L2400/06A61L2430/02C08L89/06A61L27/44A61L27/3633A61L27/365A61L27/3687A61L27/3691A61L27/446A61L2300/412
Inventor 俞春华廖化
Owner HANGZHOU HUAMAI MEDICAL DEVICES CO LTD
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