10 results about "Dental pulp cell" patented technology

Provided are: a dental curable composition that contains a radically polymerizable monomer component (A), an inorganic powder / granules (B), and a polymerization initiator (C), the dental curable composition having paste characteristics such as the viscosity decreasing greatly when the composition is heated from room temperature to 42°C, which is thought not to damage dental pulp cells; and a method for producing the dental curable composition. The dental curable composition contains 120-300 parts by mass of an inorganic powder / granules (B1) per 100 parts by mass of (A), in which the amount of monomers having a urethane group is 50 mass% or greater and the amount of monomers having an aromatic ring is less than 30 mass%. In the dental curable composition, (B1) is dispersed in (A) so that the surface of (B1) is in contact with (a1). (B) includes (B1), (B1) being obtained by surface treating the surface of (b1), which has a primary particle size of 200-1000 nm, using a silane coupling agent (D) containing a given proportion of an organic silicon compound "that is a monovalent organic group having an aromatic ring, does not contain any amino groups, and either does not contain any imino groups or hydroxyl groups or contains one of an imino group or a hydroxyl group," such as N-phenyl-3-aminopropyltrimethoxysilane.

To provide a hardenable composition comprising an encapsulated material.SOLUTION: The encapsulated material comprises a basic core material and an inorganic shell material having certain viscosity criteria. Also described are dispensing devices and kits are described comprising a hardenable composition comprising a liquid material and an encapsulated material, the encapsulated material comprising a basic core material and an inorganic shell material comprising a metal oxide surrounding the core. The dispensing devices and kits can facilitate methods of applying the hardneable composition. The hardenable or hardened composition can provide various technical effects such as a delayed release of a basic core material, a delayed increase in basicity, promoting remineralization of a tooth or bone structure, and increasing average alkaline phosphatase (ALP) activity of pulp cells. In some embodiments, the composition is a dental composition for application to a tooth structure.SELECTED DRAWING: Figure 1

The invention discloses a composition for inducing dental pulp cell reprogramming, the composition comprises a TGF-beta pathway inhibitor, an MEK inhibitor, a GSK3 inhibitor, coptisine, astragaloside and artemisinin, and the components have a synergistic effect to realize the dental pulp cell reprogramming; the invention further discloses a reprogramming culture medium composed of the composition and an alpha-MEM basic culture medium. The invention also discloses a reprogramming method for reprogramming the dental pulp cells by using the reprogramming culture medium, the method comprises the steps of dental pulp cell culture, cell inoculation, reprogramming culture and the like, and the induced pluripotent stem cells can be obtained by culturing the dental pulp cells in the reprogramming culture medium for 14 days. The composition disclosed by the invention is definite in component, the reprogramming efficiency of the dental pulp cells can be remarkably improved, and the reprogrammed induced pluripotent stem cells are good in dryness and high in hereditary stability, and can be widely applied to the cell reprogramming fields such as regenerative medicine, oral disease treatment and drug development.

The invention also discloses application of the pharmaceutical composition containing the oncolytic virus and the odontogenic stem cells in preparation of drugs for treating tumors, inhibiting tumor metastasis and / or preventing tumor recurrence. The medicine is an intravenous administration dosage form.

The invention belongs to the field of biomedical materials and periodontal tissue engineering, and relates to an injectable anti-oxidation mechanical buffer porous hydrogel microsphere delivery odontogenic stem cell system as well as a preparation method and application of the injectable anti-oxidation mechanical buffer porous hydrogel microsphere delivery odontogenic stem cell system. The microsphere is constructed by adopting a dynamic bond-covalent bond dual-network structure, has injectability, stress relaxation and mechanical support properties, and introduces a polyphenol functional group to remove active oxygen and regulate and control an inflammatory microenvironment. The communicated porous microspheres with controllable particle sizes are obtained through emulsification balling and freezing pore-forming processes and are used for loading the odontogenic stem cells to form a regeneration delivery system. The system can improve the dryness and osteogenic differentiation capacity of the odontogenic stem cells, reduce oxidative stress and inflammatory response, promote alveolar bone defect repair and bone mass recovery under the conditions of inflammation and orthodontic pressure stress, and has good biocompatibility and application prospects.

The invention provides a separation and preparation method of dental pulp stem cells. The method comprises the following steps: using dental stem cells (DSC) separated from dental pulp tissues of human permanent teeth or deciduous teeth; obtaining a single-cell suspension through a double-enzyme digestion method; washing the single-cell suspension and the phosphoric acid buffer salt solution with the same volume for three times; carrying out adherent culture on the undigested tissues; culturing in an improved MEM culture medium (MEM-alpha) supplemented with fetal calf serum (FBS) with the volume fraction of 20%, penicillin with the volume fraction of 100 g / ml and streptomycin with the volume fraction of 100 g / ml in an incubator at the temperature of 37 DEG C under the condition of 5% CO2; according to the double-enzyme digestion method, the culture medium is replaced every two days; the culture medium is replaced once every 4-5 days by the tissue adherence method; after 90% confluence is achieved, performing trypsin digestion to harvest adherent cells; under the condition, the content of CD117 positive cells exceeds 96.67%. The dental pulp stem cells obtained by the method do not induce premature differentiation of cells, the cell proliferation speed is greatly increased, more sufficient nutrition is provided for the cells, synthesis of protein is promoted, proliferation of the cells is promoted, and differentiation of the cells is inhibited; the separation speed and the separation effect are improved, and the operation difficulty is also reduced. By utilizing the method disclosed by the invention, a rapid and stable separation method and an efficient amplification system can be established, and the method has a very wide clinical application prospect in the fields of life science and medicine. Along with continuous development of research, hDPSCs can play a greater role in the medical field.

The invention discloses an irisin-based live marrow preservative and a preparation method and application thereof, the irisin-based live marrow preservative comprises irisin, cerium-containing mesoporous bioactive glass nanoparticles and hydrogel, the irisin is loaded on the mesoporous structure of the cerium-containing mesoporous bioactive glass nanoparticles, and the hydrogel is loaded on the mesoporous structure of the cerium-containing mesoporous bioactive glass nanoparticles. And hydrogel is filled among the cerium-containing mesoporous bioactive glass nanoparticles loaded with the irisin. The Irisin / Ce-MBGNs live marrow preservative disclosed by the invention is stable in physical and chemical properties, and shows good Irisin slow release and enrichment capabilities. In-vitro cell experiments and animal experiments prove that the Irisin / Ce-MBGNs live pulp preservative can regulate and control polarization of macrophages by reducing overexpressed ROS (reactive oxygen species) in a targeted manner, so that the pulpitis reaction is remarkably relieved, and the Irisin / Ce-MBGNs live pulp preservative has the effect of positively promoting dentin differentiation of dental pulp cells.

To provide a composition for promoting the regeneration of periosteum and bone.SOLUTION: The composition for regenerating periosteum and bone contains a culture supernatant of mesenchymal cells. Examples of the culture supernatant of mesenchymal cells include a culture supernatant of a mesenchymal cell population containing dental pulp cells, dental pulp stem cells, and the like.SELECTED DRAWING: None

A medium-based method for inducing specific differentiation of dental stem cells into dopaminergic neurons is provided. The method includes seeding the dental stem cells at a concentration of 5000 cells / cm2, following 24-hour incubation, introducing the cells into first part neurogenic induction medium and continuing the medium application for 4 days; subsequently, introducing the cells into the second part neurogenic induction medium and continuing the medium application for 2 days; and terminating the differentiation at the end of 6 days. The objective of the present invention is to develop cellular applications for use in treatment of neurodegenerative diseases and medications related to the said diseases.

A serum-free endothelial cell differentiation culture medium including (a) a basal culture medium and (b) an endothelial cell differentiation combination of EGF, FGF and VEGF protein. In the culture medium, the amount of EGF is higher than the amount of FGF protein. A process for the preparation of cellular aggregate suspensions comprising differentiated endothelial cells from dental stem cells using the serum-free medium. The use of the resulting suspension in therapy.