Prepn process of complicated tissue organ precursor

Abstract

The present invention is preparation process of complicated tissue organ precursor and belongs to the field of biological tissue engineering technology. Seed cell or gene and matrix material are prepared into micro colloid bead or 3D multi-canal structure; and cell and rack are located in space accurately via fast computer aided formation, coating, injecting, etc. The said process is superior to available tissue engineering process, which is time consuming and has uneven distribution of cells in rack, hard permeation of cell to deep structure and other demerits. By means of part combination, the present invention can reach the requirement of complicated organ, and realize organ reconstruction for repair and regeneration.

Description

technical field [0001] The invention relates to a method for preparing complex tissue and organ precursors, belonging to the technical field of biological tissue engineering. Background technique [0002] There are tens of millions of patients suffering from tissue defect or organ failure every year in the world, and about 8 million of these patients are treated with surgery every year in the United States alone. However, living donor organs are limited, and existing mechanical devices do not have all the functions of the organs and cannot prevent further deterioration of the patient's condition. Accordingly, Tissue Engineering (Tissue Engineering) technology with the purpose of improving the treatment level of such diseases came into being. Tissue engineering is a high-tech discipline produced by the intersection of biology, medicine, materials science, engineering and other disciplines. Its meaning is to apply the principles and technologies of life science and engineeri...

AI Technical Summary

Problems solved by technology

However, the existing tissue engineering technology faces many difficulties and limitations. The success of tissue engineering application research is in those tissues and organs with relatively simple structure and physiological functions, such as bone, cartilage, skin, etc.

The traditional stent preparation technology cannot accurately control the size, structure, spatial distribution and through channels of the pores, and the supply of nutrients and the growth of blood vessels are greatly restricted.

The traditional tissue engineering method generally prepares the structural scaffold first. During the cell culture process, the cells in the upper layer consume most of the oxygen and nutrients, which limits the diffusion of these components to the bottom layer, thereby limiting the migration of cells to the deep layer of the scaffold, etc.

This method of preparing the scaffold first and then culturing the cells is time-consuming and laborious. The cells are likely to be deformed and aged during the process of migrating into the scaffold, which cannot meet the requirements of timely treatment of clinical patients.

At the same time, traditional tissue engineering technology cannot meet the needs of accurately positioning and placing different cells in space and constructing functionally graded structures of complex tissues and organs.