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Construction method of swine-origin collagen membrane-autologous chondrocyte composite scaffold

A technology of chondrocytes and composite scaffolds, which is applied in the direction of bone/connective tissue cells, cell culture support/coating, animal cells, etc., can solve the problems of poor treatment effect, achieve good treatment effect, large repair area, and simplified The effect of the surgical procedure

Inactive Publication Date: 2017-07-07
安徽安龙基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a method for constructing a porcine collagen membrane-autologous chondrocyte composite scaffold to solve the above-mentioned problems in order to solve the defects of poor therapeutic effect in the prior art

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The invention discloses a method for constructing a pig-derived collagen membrane-autologous chondrocyte composite scaffold, and the specific steps are as follows:

[0063] (1) Take about 300 mg of articular cartilage in the non-weight-bearing area of ​​the patient, remove the clean soft tissue, put the remaining transparent cartilage tissue pieces into a centrifuge tube filled with PBS buffer, shake and wash until the residual blood is rinsed away;

[0064] (2) In a sterile plate, cut the cartilage tissue into pieces of 1 mm with ophthalmic scissors 3 fragments of

[0065] (3) Digest the above fragments with 0.25% (m / v) trypsin for 30 minutes, and discard the supernatant;

[0066] (4) Digest the fragments with 5ml of 0.02% (m / v) type II collagenase at 37°C in a 5% CO2 incubator for 8 hours, shake once every 1 hour until they become flocculent, and obtain cell suspensions. liquid;

[0067] (5) Filter the cell suspension with a 100um filter, add FBS-containing high-su...

Embodiment 2

[0084] The invention discloses a method for constructing a pig-derived collagen membrane-autologous chondrocyte composite scaffold, and the specific steps are as follows:

[0085](1) Take about 250 mg of articular cartilage in the non-weight-bearing area of ​​the patient, remove the clean soft tissue, put the remaining transparent cartilage tissue into a centrifuge tube filled with PBS buffer, shake and wash until the residual blood is rinsed away;

[0086] (2) In a sterile plate, cut the cartilage tissue into pieces of 1 mm with ophthalmic scissors 3 fragments of

[0087] (3) Digest the above fragments with 0.25% (m / v) trypsin for 30 minutes, and discard the supernatant;

[0088] (4) Digest the fragments with 5ml of 0.02% (m / v) type II collagenase at 37°C and 5% CO2 incubator for 9 hours, and shake once every hour until they become flocculent, and the cell suspension is obtained. liquid;

[0089] (5) Filter the cell suspension with a 100um filter, add FBS-containing high-s...

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Abstract

The invention discloses a method for constructing a composite scaffold of pig-derived collagen membrane-autologous chondrocytes. The specific steps are as follows: take the patient's articular cartilage, shake and clean it; cut the cartilage tissue into pieces; obtain cell suspension; precipitate as chondrocytes; Dried the porcine type Ⅰ / Ⅲ collagen membrane, placed it in a sterile plastic plate, slowly dripped the cell suspension on the rough surface of the prepared porcine type Ⅰ / Ⅲ collagen membrane with a plastic sleeve until the collagen membrane reached Wet and saturated state; wait for 10-15 minutes after the collagen membrane adsorbs the cells. The invention has the advantages of good therapeutic effect and the like.

Description

technical field [0001] The invention relates to the field of tissue engineering articular cartilage damage repair treatment, in particular to a method for constructing a porcine collagen membrane-autologous chondrocyte composite scaffold. Background technique [0002] Joint problems are relatively common and occur at all ages. Articular cartilage defects can lead to injury. Joint injuries can be caused by movement beyond normal, or from overuse, muscle weakness, and general wear and tear. Different cartilage defects require different treatments . [0003] At present, the commonly used treatment methods for cartilage defects include bone marrow stimulation, allogeneic osteochondral transplantation, allogeneic chondrocyte transplantation, autologous chondrocyte transplantation, tissue engineering repair, etc., but there are still various problems in these techniques. [0004] The bone marrow stimulation method destroys the continuity of the subchondral bone at the cartilage d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/50A61L27/54A61L27/58C12N5/077
CPCA61L27/3817A61L27/24A61L27/3852A61L27/50A61L27/54A61L27/58A61L2300/412A61L2430/06C12N5/0655C12N2533/54
Inventor 韦玉军李航陆宝石苏军吴远航
Owner 安徽安龙基因科技有限公司
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