44 results about "Swine origin" patented technology

The invention provides application of a porcine parainfluenza virus type 5 as an expression vector, and belongs to the technical field of biological vaccines. According to the invention, a PIV5 strain separated from diarrhea pigs in Jiangsu Province in China is utilized to construct PIV5 virus infectious clone. The eGFP gene is inserted into a PIV5 infectious cloned virus genome, the recombinant PIV5 virus capable of expressing the green fluorescent protein is successfully rescued, and when the eGFP gene is inserted into a P / M gene spacer region, the expression quantity is the highest, which indicates that an exogenous gene expression cassette can influence the expression level of an exogenous gene, and the P / M gene spacer region is the optimal site for inserting the exogenous gene, so that the recombinant PIV5 virus can be successfully rescued. And a solid foundation is laid for developing a vector vaccine by utilizing the swine PIV5. The porcine parainfluenza virus type 5 can be used as an expression vector to express foreign proteins, can also be used for preparing oral vaccines, and can relieve the stress reaction of pigs in the immune process.

The invention belongs to the field of vaccine microorganisms and application thereof, and particularly relates to screening of an A-type pasteurella multocida vaccine strain and application of the strain. According to the invention, a strain of Pasteurella multocida PmA-115 is separated and screened, and the Pasteurella multocida PmA-115 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Pasteurella multocida PmA-115 is CCTCC NO: M 20242428. The strain can be applied to preparation of pig pasteurella multocida vaccines.

The present invention belongs to the field of biotechnology, and discloses a Bacillus subtilis strain from Tibetan pigs, with the classification name of Bacillus subtilis Yirun2024, the Latin name of Bacillus subtilis Yirun2024, the preservation number of CCTCC NO: M 20241752, the preservation date of August 7, 2024, the preservation unit of China Center for Type Culture Collection, and the preservation address of Wuhan University, Wuhan, China. This Bacillus subtilis has high cellulase production. After 12 h of fermentation in a shake flask, the activities of endoglucanase, exoglucanase and β-glucosidase are respectively as high as 2902.72 U / mL, 11026.33 U / mL and 2983.04 U / mL, far exceeding those of similar engineering bacteria. At the same time, the present invention also provides the application of this Bacillus subtilis, which is of great significance in promoting the application field of fiber resources. At the same time, this strain can maintain a relatively high survival rate in a high-temperature environment.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The present invention relates to a strain of Clostridium butyricum and its application, belonging to the field of microbiology. The Clostridium butyricum JBH-BD1 of the present invention is preserved in the China Center for Type Culture Collection, with the preservation number CCTCC NO: M2022151. The Clostridium butyricum of the present invention is natural, safe, acid-resistant, alkali-resistant, heat-resistant, and has strong abilities to produce protease, amylase and organic acids. At the same time, it has antibacterial effects on Escherichia coli, Salmonella, Staphylococcus aureus, Clostridium perfringens, Pasteurella multocida of porcine origin and Actinobacillus pleuropneumoniae, and can convert inorganic ferrous iron into organic ferrous iron; when used in the aquaculture industry, it can not only regulate the balance of intestinal flora, promote the growth of livestock and poultry, but also effectively improve the utilization rate of iron element, reduce its addition amount, prevent iron deficiency anemia in livestock and poultry, improve the absorption and utilization efficiency of iron by livestock and poultry, and increase the aquaculture benefits.

The invention discloses swine bacillus subtilis TZ1202 and application thereof, and belongs to the technical field of genetic engineering. The preservation number of the bacillus subtilis TZ1202 is CGMCC (China General Microbiological Culture Collection Center) No.33665, and the 16S rRNA (Ribose Nucleic Acid) sequence of the bacillus subtilis TZ1202 is shown as SEQ ID NO.1. The bacillus subtilis TZ1202 carries toxin genes such as hblA, hblC and the like, and is sensitive to 20 antibiotics and resistant to oxacillin. Animal experiments prove that the strain has pathogenicity / lethality to piglets and mice. The invention also provides a preparation method of an inactivated vaccine of the strain. An efficacy test shows that 0.25 mL of the strain can completely protect mice. In conclusion, the strain provided by the invention can be used for development of porcine epidemic disease quarantine reference substances and vaccines, and the detection accuracy and the prevention and treatment effect are remarkably improved.

The invention relates to the technical field of animal breeding, in particular to an expression vector based on a skin specific promoter and application of the expression vector in preparation of black skin pigs. The expression vector comprises an expression cassette, and the expression cassette comprises a 5'end regulation region of a pig source K5 gene, an intron of a beta-globin gene, a target gene and a 3 'end regulation region of a K14 gene. An expression vector for specifically expressing a target gene in animal skin is provided by adopting a regulatory region of a porcine K5 gene and an intron of a beta-globin gene, and the target gene (such as a melanin synthesis regulatory gene) is integrated by adopting the expression vector and can be efficiently expressed in a porcine somatic cell so as to prepare a pig with specific characters; the method has an important application value in the pig raising industry.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The invention discloses application of a pig SOS1 gene in regulation and control of adipocyte differentiation, and relates to the technical field of biology. An overexpression system is constructed by exogenously introducing a swine SOS1 gene into 3T3-L1 precursor fat cells of a mouse, and the influence of the overexpression system on adipogenic differentiation of the cells is detected; researches find that the overexpression of the pig SOS1 gene significantly promotes the adipogenic differentiation of 3T3-L1 cells of mice, and improves the mRNA and protein expression levels of key adipogenic markers PPAR gamma, FABP4 and HSL. The invention provides a theoretical basis for improving the fat deposition character of the pig.

The invention discloses a porcine circovirus type 4 Cap protein antigen epitope peptide and a monoclonal antibody thereof. The amino acid sequence of the antigen epitope peptide is as shown in SEQ ID No. 11; the heavy chain variable region of the monoclonal antibody comprises CDR1-3 as shown in SEQ ID No.1 to SEQ ID No.3; the light chain variable region comprises CDR1-3 as shown in SEQ ID No. 4 to 6; the monoclonal antibody does not react with a plurality of other swine viruses such as PCV2, PCV3, PEDV, PRRSV and the like, and has good specificity; the antibody can be obtained by biological means such as conventional genetic engineering, and the problem that the genetic sequence of the antibody is easy to lose due to long-term cryopreservation of traditional hybridoma cells is avoided; optimization of the antibody on the gene or protein level is facilitated, and the specificity and affinity of the antibody are further improved.

The invention belongs to the field of vaccine microorganisms, and provides a swine pasteurella multocida attenuated strain PmA4-750, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251644. The invention further provides a preparation method of the swine pasteurella multocida attenuated strain PmA4-750. After alternate high-temperature continuous passage, three generations of whole-gene sequencing and pathogenicity contrast tests find that a vaccine candidate strain (PmA4-750 strain) has a section of specific insertion fragment with the length of about 43kb, and 14 genetic variation sites, including 10 InDel (insertion / deletion) and 4 SNP (single nucleotide polymorphism), exist in the vaccine candidate strain. The inserted genes may generate adaptive evolution of strains in the passage process of the strains, so that the pathogenic characteristics of the strains are changed, and the toxicity is promoted to be remarkably reduced. In addition, a piglet immune challenge protection test result shows that the PmA4-750 strain has an immune effect on swine pasteurellosis caused by type A, type D or type B pasteurella multocida. In addition, the invention also discloses a swine pasteurellosis live vaccine and a preparation method thereof.

The invention discloses an anti-African swine fever p72 protein single-chain antibody as well as a nucleic acid molecule, a recombinant expression vector, a preparation method and application thereof, and belongs to the technical field of detection of pathogenic microorganisms of livestock. The single-chain antibody is formed by sequentially connecting a heavy chain variable region, a connecting peptide and a light chain variable region, and the amino acid sequence of the single-chain antibody is shown as SEQ ID NO: 1. The invention also provides a nucleic acid molecule for coding the single-chain antibody as well as a recombinant expression vector and a host cell of the nucleic acid molecule. The single-chain antibody with high affinity and high specificity is obtained by screening through a phage display technology, is only combined with the African swine fever virus p72 protein, does not react with common swine virus protein, is small in molecular weight and good in stability, is suitable for various detection scenes such as immunodetection and histochemical analysis, and has good application prospects. The kit can be used for developing products such as a rapid diagnostic kit and a lateral flow test strip, and is suitable for detecting African swine fever.

The present invention provides probiotic compositions comprising isolated bacterial strains, referred to herein as LactX, StrepX1, and StrepX3. Methods of using the compositions to increase the growth rate of pigs, to alleviate the detrimental growth effects of weaning pigs, and to reduce the time from birth to marketable weight for pigs are also provided.

The invention discloses a primer probe combination and a kit for on-site detection of pig, horse, duck, cattle and sheep derived components, and relates to the technical field of gene detection. The invention provides a primer probe combination for on-site detection of pig, horse, duck, cattle and sheep-derived components. The primer probe combination comprises a primer pair and a probe for detecting the pig-derived components, the primer pair and the probe are used for detecting horse source components; a primer pair and a probe for detecting the duck-derived component; the primer pair and the probe are used for detecting bovine-derived components; the invention relates to a primer pair and a probe for detecting sheep-derived components. The method for detecting the pig, horse, duck, cattle and sheep derived components by adopting the primer probe combination and the kit integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has better sensitivity and accuracy, is simple and convenient to operate, is quick and time-saving, and has a good application prospect in detection of pig, horse, duck, cattle and sheep derived components. The method plays an important role in on-site rapid detection of pig, horse, duck, cattle and sheep source component research and safety management.

The application discloses a broad-spectrum neutralizing pig-derived monoclonal antibody of type A foot-and-mouth disease virus (FMDV) and application thereof. The antibody PAF56 has an amino acid sequence of a heavy chain variable region as shown in SEQ ID No. 12, a heavy chain CDR1 sequence of GFGFSNTY, a heavy chain CDR2 sequence of VFTGGGAT, and a heavy chain CDR3 sequence of VSNRDGFGGVGNV. The antibody PAF56 has an amino acid sequence of a light chain variable region as shown in SEQ ID No. 13, a light chain CDR1 sequence of SGSVTSLNY, a light chain CDR2 sequence of RTN, and a light chain CDR3 sequence of ALYKGSGTDTNI. The antibody PAF56 can simultaneously neutralize an AF72 strain of an A22 lineage and A / WH / CHA / 09 and A / GDMM / 2013 epidemic strains of a domestic SEA97 lineage, and has broad-spectrum neutralization activity of type A FMDV. The antibody PAF56 recognizes an antigen site 2 of a VP2 B-C loop, and a key amino acid is VP2 D72; the recognized site is highly conserved in type A FMDV. The application first obtains a broad-spectrum neutralizing antibody of type A specific FMDV from a natural host and identifies a key antigen site recognized by the antibody, thereby providing a powerful tool for antigen structure analysis of FMDV and establishment of a serological detection method.

The invention discloses a primer probe combination and a kit for fluorescent quantitative PCR (Polymerase Chain Reaction) detection of African swine fever virus and application of the primer probe combination and the kit. The primer probe combination is a specific primer probe combination 1 or a specific primer probe combination 2. The nucleotide sequence of the specific primer probe combination 1 is as shown in SEQ ID NO.1-3, and the nucleotide sequence of the specific primer probe combination 2 is as shown in SEQ ID NO.4-6. The qPCR detection method with strong specificity and high sensitivity is established, the lower detection limit can reach 0.87 copies / L, the sensitivity of the qPCR detection method is equivalent to that of a WOAH recommendation method, and the detection rate of weak positive samples is higher; the kit has no cross reaction with common swine pathogens such as hog cholera virus and pseudorabies virus, and has good specificity. The method provides reliable technical support for early diagnosis and precise prevention and control of African swine fever.

The invention provides a primer, a probe, a kit and a method for detecting swine pathogenic escherichia coli. Specifically, a fluorescent quantitative PCR method is utilized, swine escherichia coli chuS and kpsTII are taken as target genes, conservative primer and probe sequences are designed, a duplex fluorescent quantitative PCR detection technology is established, and swine escherichia coli clinical strains can be distinguished into a virulent strain with double positive chuS and kpsTII, a medium virulent strain with positive chuS and kpsTII and a low virulent strain with positive chuS and kpsTII. The method is beneficial to rapid identification of pathogenic strains and guidance of formulation of clinical prevention and control strategies.

The invention discloses a cell line capable of stably expressing a porcine PIGR SC fragment and a J chain as well as a construction method and application of the cell line, and belongs to the technical field of gene engineering. The construction method comprises the following steps: inserting coding sequences of an SC fragment gene and a J-chain gene of the porcine PIgR into lentiviral vector plasmids to obtain an SC fragment and J-chain overexpressed lentiviral vector plasmid of the PIgR; carrying out co-transfection on the lentivirus vector plasmid, PPSAX2 and PMD2. G plasmids, so as to obtain a recombinant lentivirus; and infecting cells with the recombinant lentivirus to obtain positive cells, and carrying out enlarged culture to obtain the SC fragment and J chain cell line for stably expressing the swine PIGR. According to the invention, a cell line for stably expressing the SC fragment and the J chain of the porcine PIgR is constructed, so that the SIgA antibody can be rapidly and efficiently prepared by a gene engineering technology.

This invention belongs to the field of biology and discloses a porcine Clostridium perfringens strain SP20 that secretes and expresses high levels of α-toxin. It is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2025386. This bacterium exhibits strong pathogenicity in medium and large-sized pigs and can be used to establish porcine challenge models and evaluate the efficacy of porcine Clostridium perfringens type A vaccines. This invention also discloses a vaccine for preventing intestinal bloating and sudden death in medium and large-sized pigs caused by Clostridium perfringens type A. This vaccine contains α-toxin produced by the aforementioned SP20 strain. This invention fills the gap in the development of pathogenic models for intestinal bloating and sudden death in medium and large-sized pigs, which is beneficial for controlling disease transmission and reducing economic losses in the livestock industry.

The invention discloses a primer probe combination and a kit for on-site detection of pig, horse, duck, cattle and yak source components, and relates to the technical field of gene detection. The invention provides a primer probe combination for detecting pig, horse, duck, cattle and yak source components on site. The primer probe combination comprises a primer pair and a probe for detecting pig source components, the primer pair and the probe are used for detecting horse source components; a primer pair and a probe for detecting the duck-derived component; the primer pair and the probe are used for detecting cattle-derived components; the invention discloses a primer pair and a probe for detecting yak-derived components. The method for detecting pig, horse, duck, cattle and yak source components by adopting the primer probe combination and the kit integrates sample pretreatment, nucleic acid extraction, purification, amplification and detection, has relatively good sensitivity and accuracy, is simple and convenient to operate, is rapid and time-saving, and has a good application prospect in detection of pig, horse, duck, cattle and yak source components. And the method plays an important role in on-site rapid detection of pig, horse, duck, cattle and yak source component research and safety management.

The present application discloses a chimeric classical swine fever virus, a classical swine fever marker vaccine, and a preparation method and application thereof. In the embodiment of the present application, the non-coding region and the main antigenic region of the E2 protein of the attenuated live classical swine fever vaccine strain C were replaced with the corresponding region of the BVDV Hubei strain by reverse genetic manipulation technology, and an infectious clone of the chimeric classical swine fever virus was constructed. The infectious clone was rescued by cell transfection and passage to obtain a chimeric classical swine fever virus with stable characteristics. The chimeric classical swine fever virus can stably replicate in pig-derived cells and has genetic stability and safety; it can induce the production of specific antibodies after immunization of the body, and can also provide the pig with complete protection against lethal infection by the virulent classical swine fever virus. In addition, the strain can immunize to produce BVDV E2-specific antibodies to distinguish between vaccinated pigs and wild-type classical swine fever virus-infected pigs, so that the strain has an application prospect as a classical swine fever marker vaccine.

The invention provides a pig source leuconostoc citreum (Leuconostoc citreum) with the effects of reducing emission and increasing weight and an application of the pig source leuconostoc citreum. The microorganism preservation number of the leuconostoc citreum S4 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.31017. The strain provided by the invention is separated and screened from healthy pig manure, and has the characteristics of producing acid and exopolysaccharides. The invention discloses an optimized culture medium of a strain. The culture medium can improve the culture efficiency and polysaccharide yield of the strain. The invention further discloses a fermented feed containing the leuconostoc citreum S4, the fermented feed is added into a fattening pig feed, the daily gain of a pig herd can be remarkably increased, emission of fecal odor substances and escherichia coli in excrement can be remarkably reduced, and green and efficient development of pig breeding can be promoted.

The invention discloses a foot-and-mouth disease virus type O specific neutralizing swine monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.2. The invention also provides application of the monoclonal antibody in preparation of a reagent or a kit for detecting the O-type foot-and-mouth disease virus and a medicine for preventing O-type foot-and-mouth disease virus infection. The antibody is a whole swine source, is good in safety, can specifically neutralize O-type FMDV PanAsia, Mya98 and Cathay classical pedigree strains, identifies key antigen sites as 73rd and 134 residues of VP3, can be used for O-type FMDV differential diagnosis and broad-spectrum vaccine design, and has an important application value.

The present invention provides probiotic compositions comprising isolated bacterial strains, referred to herein as LactX, StrepX1 and StrepX3. Methods of using the compositions to increase growth rate in pigs and enhance pig production are also provided.

This invention discloses a porcine lactobacillus mucosa HEB-FEC-LM-001 and its applications, belonging to the field of feed additive technology. The porcine lactobacillus mucosa HEB-FEC-LM-001 provided by this invention is a Gram-positive bacterium with a milky-white, raised colony appearance, clear edges, and a smooth surface. It was deposited at the China Center for Type Culture Collection on October 21, 2025, with accession number CCTCC NO:M 20252272. A synbiotic is prepared by combining porcine lactobacillus mucosa HEB-FEC-LM-001 with raffinose. When fed as a feed additive to weaned piglets, it can significantly improve the piglets' intestinal health, apparent nutrient digestibility, and immune function, significantly reduce the diarrhea rate, and improve the growth performance of weaned piglets. Therefore, it has broad application prospects as a feed additive for weaned piglets.

The invention discloses an application of N-[4-(cinnamamido) butyl] benzamide in preparation of a TLR7 ligand. The invention finds that N-[4-(cinnamamido) butyl] benzamide can be used as a ligand of TLR7, and has strong binding and interaction with swine TLR7 protein. Therefore, the N-[4-(cinnamamido) butyl] benzamide can be used for preparing a TLR7 ligand or a TLR7 immunomodulator of an animal; besides, as the TLR7 protein is conservative in evolution, the N-[4-(cinnamamido) butyl] benzamide is not limited to pigs in application and can also be applied to other species.

The present application relates to the technical field of Bacillus amyloliquefaciens, and discloses a pig-derived broad-spectrum bacteriostatic Bacillus amyloliquefaciens and application thereof; the Bacillus amyloliquefaciens provided by the present application is Bacillus amyloliquefaciens Y106-A1-3. The bacterium is isolated from the excrement of healthy pigs, and the gastrointestinal tolerance test proves that the bacterium has strong gastrointestinal tolerance; and experiments prove that the fermentation supernatant of the Bacillus amyloliquefaciens has a bacteriostatic effect and a wide bacteriostatic spectrum, and can be used for regulating the intestinal microecological balance of animals, preventing diseases and improving the growth and production performance of animals. The microbial preparation containing the Bacillus amyloliquefaciens and the related components of the fermentation liquor has very important significance for reducing the use of antibiotics, improving the quality of agricultural and livestock products and protecting the environment, and has a good application prospect.

The invention belongs to the field of biological detection, and particularly relates to an sgRNA and RPA primer group for synchronously detecting four porcine-derived viruses, a detection system and application. The sgRNA and RPA primer group for synchronously detecting the four porcine-derived viruses comprises a sgRNA and RPA primer group for detecting a porcine acute diarrhea syndrome coronavirus, a porcine rotavirus, a porcine transmissible gastroenteritis virus and a porcine epidemic diarrhea virus. The invention further provides a centrifugal micro-fluidic chip for synchronously detecting the four swine-derived viruses, the chip can be used for synchronously and highly specifically discriminating various swine-derived viruses and accurately distinguishing infection types in a single detection process, and a detection result has laboratory-level sensitivity and accuracy.

The invention provides a primer group, a detection method of national standard swine-derived components and application, and relates to the technical field of biological detection. The invention provides a primer group. The primer group comprises a primer pair and a DBQ dual-extraction probe, wherein the primer pair and a conserved region of a national standard pig ATP8 gene have specificity and complementarity. The primer group provided by the invention aims at a conserved region of the national standard pig ATP8 gene, high specificity and accuracy of qPCR detection are ensured, and the non-specific amplification risk is effectively reduced. The high specificity of the primer pair enables the primer pair to be capable of detecting national standard swine-derived components in a trace sample, and the DBQ double-quenching probe realizes real-time monitoring of amplification of a target DNA sequence, so that the accuracy of quantitative analysis is improved. The combination of the primer pair and the probe reduces false positive and false negative results, is crucial to food safety detection and supervision, and ensures the reliability of detection results.

The invention belongs to the technical field of preparation of microorganisms and vaccines thereof, and relates to an F-type pasteurella multocida strain and an application of the F-type pasteurella multocida strain in preparation of vaccines, the strain is classified and named as pasteurella PmF-19 (Pasteurella sp.F-19), the strain is preserved in China Center for Type Culture Collection on October 30, 2025, and the preservation number is CCTCC NO: M 20252391. The pig pasteurella multocida provided by the invention has very strong toxicity, the vaccine prepared by inactivation of the pig pasteurella multocida has good safety, the vaccine generates high-level antibodies after immunizing mice and pigs, and the vaccine has cross protection force on F and D serotype pasteurella multocida infection in the mice, and can be used for immunizing the mice and pigs. Meanwhile, complete protection of F-type strains can be provided for pigs, and the pigs can resist attack of F-type Pm lethal dose.