7 results about "Swine origin" patented technology

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The invention discloses an anti-African swine fever p72 protein single-chain antibody as well as a nucleic acid molecule, a recombinant expression vector, a preparation method and application thereof, and belongs to the technical field of detection of pathogenic microorganisms of livestock. The single-chain antibody is formed by sequentially connecting a heavy chain variable region, a connecting peptide and a light chain variable region, and the amino acid sequence of the single-chain antibody is shown as SEQ ID NO: 1. The invention also provides a nucleic acid molecule for coding the single-chain antibody as well as a recombinant expression vector and a host cell of the nucleic acid molecule. The single-chain antibody with high affinity and high specificity is obtained by screening through a phage display technology, is only combined with the African swine fever virus p72 protein, does not react with common swine virus protein, is small in molecular weight and good in stability, is suitable for various detection scenes such as immunodetection and histochemical analysis, and has good application prospects. The kit can be used for developing products such as a rapid diagnostic kit and a lateral flow test strip, and is suitable for detecting African swine fever.

The invention discloses a foot-and-mouth disease virus type O specific neutralizing swine monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.2. The invention also provides application of the monoclonal antibody in preparation of a reagent or a kit for detecting the O-type foot-and-mouth disease virus and a medicine for preventing O-type foot-and-mouth disease virus infection. The antibody is a whole swine source, is good in safety, can specifically neutralize O-type FMDV PanAsia, Mya98 and Cathay classical pedigree strains, identifies key antigen sites as 73rd and 134 residues of VP3, can be used for O-type FMDV differential diagnosis and broad-spectrum vaccine design, and has an important application value.

This invention discloses a porcine lactobacillus mucosa HEB-FEC-LM-001 and its applications, belonging to the field of feed additive technology. The porcine lactobacillus mucosa HEB-FEC-LM-001 provided by this invention is a Gram-positive bacterium with a milky-white, raised colony appearance, clear edges, and a smooth surface. It was deposited at the China Center for Type Culture Collection on October 21, 2025, with accession number CCTCC NO:M 20252272. A synbiotic is prepared by combining porcine lactobacillus mucosa HEB-FEC-LM-001 with raffinose. When fed as a feed additive to weaned piglets, it can significantly improve the piglets' intestinal health, apparent nutrient digestibility, and immune function, significantly reduce the diarrhea rate, and improve the growth performance of weaned piglets. Therefore, it has broad application prospects as a feed additive for weaned piglets.

The invention belongs to the technical field of preparation of microorganisms and vaccines thereof, and relates to an F-type pasteurella multocida strain and an application of the F-type pasteurella multocida strain in preparation of vaccines, the strain is classified and named as pasteurella PmF-19 (Pasteurella sp.F-19), the strain is preserved in China Center for Type Culture Collection on October 30, 2025, and the preservation number is CCTCC NO: M 20252391. The pig pasteurella multocida provided by the invention has very strong toxicity, the vaccine prepared by inactivation of the pig pasteurella multocida has good safety, the vaccine generates high-level antibodies after immunizing mice and pigs, and the vaccine has cross protection force on F and D serotype pasteurella multocida infection in the mice, and can be used for immunizing the mice and pigs. Meanwhile, complete protection of F-type strains can be provided for pigs, and the pigs can resist attack of F-type Pm lethal dose.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.