25 results about "Swine origin" patented technology

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The invention relates to the technical field of animal breeding, in particular to an expression vector based on a skin specific promoter and application of the expression vector in preparation of black skin pigs. The expression vector comprises an expression cassette, and the expression cassette comprises a 5'end regulation region of a pig source K5 gene, an intron of a beta-globin gene, a target gene and a 3 'end regulation region of a K14 gene. An expression vector for specifically expressing a target gene in animal skin is provided by adopting a regulatory region of a porcine K5 gene and an intron of a beta-globin gene, and the target gene (such as a melanin synthesis regulatory gene) is integrated by adopting the expression vector and can be efficiently expressed in a porcine somatic cell so as to prepare a pig with specific characters; the method has an important application value in the pig raising industry.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The invention discloses application of a pig SOS1 gene in regulation and control of adipocyte differentiation, and relates to the technical field of biology. An overexpression system is constructed by exogenously introducing a swine SOS1 gene into 3T3-L1 precursor fat cells of a mouse, and the influence of the overexpression system on adipogenic differentiation of the cells is detected; researches find that the overexpression of the pig SOS1 gene significantly promotes the adipogenic differentiation of 3T3-L1 cells of mice, and improves the mRNA and protein expression levels of key adipogenic markers PPAR gamma, FABP4 and HSL. The invention provides a theoretical basis for improving the fat deposition character of the pig.

The invention belongs to the field of vaccine microorganisms, and provides a swine pasteurella multocida attenuated strain PmA4-750, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251644. The invention further provides a preparation method of the swine pasteurella multocida attenuated strain PmA4-750. After alternate high-temperature continuous passage, three generations of whole-gene sequencing and pathogenicity contrast tests find that a vaccine candidate strain (PmA4-750 strain) has a section of specific insertion fragment with the length of about 43kb, and 14 genetic variation sites, including 10 InDel (insertion / deletion) and 4 SNP (single nucleotide polymorphism), exist in the vaccine candidate strain. The inserted genes may generate adaptive evolution of strains in the passage process of the strains, so that the pathogenic characteristics of the strains are changed, and the toxicity is promoted to be remarkably reduced. In addition, a piglet immune challenge protection test result shows that the PmA4-750 strain has an immune effect on swine pasteurellosis caused by type A, type D or type B pasteurella multocida. In addition, the invention also discloses a swine pasteurellosis live vaccine and a preparation method thereof.

The invention discloses an anti-African swine fever p72 protein single-chain antibody as well as a nucleic acid molecule, a recombinant expression vector, a preparation method and application thereof, and belongs to the technical field of detection of pathogenic microorganisms of livestock. The single-chain antibody is formed by sequentially connecting a heavy chain variable region, a connecting peptide and a light chain variable region, and the amino acid sequence of the single-chain antibody is shown as SEQ ID NO: 1. The invention also provides a nucleic acid molecule for coding the single-chain antibody as well as a recombinant expression vector and a host cell of the nucleic acid molecule. The single-chain antibody with high affinity and high specificity is obtained by screening through a phage display technology, is only combined with the African swine fever virus p72 protein, does not react with common swine virus protein, is small in molecular weight and good in stability, is suitable for various detection scenes such as immunodetection and histochemical analysis, and has good application prospects. The kit can be used for developing products such as a rapid diagnostic kit and a lateral flow test strip, and is suitable for detecting African swine fever.

The application discloses a broad-spectrum neutralizing pig-derived monoclonal antibody of type A foot-and-mouth disease virus (FMDV) and application thereof. The antibody PAF56 has an amino acid sequence of a heavy chain variable region as shown in SEQ ID No. 12, a heavy chain CDR1 sequence of GFGFSNTY, a heavy chain CDR2 sequence of VFTGGGAT, and a heavy chain CDR3 sequence of VSNRDGFGGVGNV. The antibody PAF56 has an amino acid sequence of a light chain variable region as shown in SEQ ID No. 13, a light chain CDR1 sequence of SGSVTSLNY, a light chain CDR2 sequence of RTN, and a light chain CDR3 sequence of ALYKGSGTDTNI. The antibody PAF56 can simultaneously neutralize an AF72 strain of an A22 lineage and A / WH / CHA / 09 and A / GDMM / 2013 epidemic strains of a domestic SEA97 lineage, and has broad-spectrum neutralization activity of type A FMDV. The antibody PAF56 recognizes an antigen site 2 of a VP2 B-C loop, and a key amino acid is VP2 D72; the recognized site is highly conserved in type A FMDV. The application first obtains a broad-spectrum neutralizing antibody of type A specific FMDV from a natural host and identifies a key antigen site recognized by the antibody, thereby providing a powerful tool for antigen structure analysis of FMDV and establishment of a serological detection method.

The invention discloses a primer probe combination and a kit for fluorescent quantitative PCR (Polymerase Chain Reaction) detection of African swine fever virus and application of the primer probe combination and the kit. The primer probe combination is a specific primer probe combination 1 or a specific primer probe combination 2. The nucleotide sequence of the specific primer probe combination 1 is as shown in SEQ ID NO.1-3, and the nucleotide sequence of the specific primer probe combination 2 is as shown in SEQ ID NO.4-6. The qPCR detection method with strong specificity and high sensitivity is established, the lower detection limit can reach 0.87 copies / L, the sensitivity of the qPCR detection method is equivalent to that of a WOAH recommendation method, and the detection rate of weak positive samples is higher; the kit has no cross reaction with common swine pathogens such as hog cholera virus and pseudorabies virus, and has good specificity. The method provides reliable technical support for early diagnosis and precise prevention and control of African swine fever.

This invention belongs to the field of biology and discloses a porcine Clostridium perfringens strain SP20 that secretes and expresses high levels of α-toxin. It is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2025386. This bacterium exhibits strong pathogenicity in medium and large-sized pigs and can be used to establish porcine challenge models and evaluate the efficacy of porcine Clostridium perfringens type A vaccines. This invention also discloses a vaccine for preventing intestinal bloating and sudden death in medium and large-sized pigs caused by Clostridium perfringens type A. This vaccine contains α-toxin produced by the aforementioned SP20 strain. This invention fills the gap in the development of pathogenic models for intestinal bloating and sudden death in medium and large-sized pigs, which is beneficial for controlling disease transmission and reducing economic losses in the livestock industry.

The invention discloses a foot-and-mouth disease virus type O specific neutralizing swine monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.2. The invention also provides application of the monoclonal antibody in preparation of a reagent or a kit for detecting the O-type foot-and-mouth disease virus and a medicine for preventing O-type foot-and-mouth disease virus infection. The antibody is a whole swine source, is good in safety, can specifically neutralize O-type FMDV PanAsia, Mya98 and Cathay classical pedigree strains, identifies key antigen sites as 73rd and 134 residues of VP3, can be used for O-type FMDV differential diagnosis and broad-spectrum vaccine design, and has an important application value.

The present invention provides probiotic compositions comprising isolated bacterial strains, referred to herein as LactX, StrepX1 and StrepX3. Methods of using the compositions to increase growth rate in pigs and enhance pig production are also provided.

This invention discloses a porcine lactobacillus mucosa HEB-FEC-LM-001 and its applications, belonging to the field of feed additive technology. The porcine lactobacillus mucosa HEB-FEC-LM-001 provided by this invention is a Gram-positive bacterium with a milky-white, raised colony appearance, clear edges, and a smooth surface. It was deposited at the China Center for Type Culture Collection on October 21, 2025, with accession number CCTCC NO:M 20252272. A synbiotic is prepared by combining porcine lactobacillus mucosa HEB-FEC-LM-001 with raffinose. When fed as a feed additive to weaned piglets, it can significantly improve the piglets' intestinal health, apparent nutrient digestibility, and immune function, significantly reduce the diarrhea rate, and improve the growth performance of weaned piglets. Therefore, it has broad application prospects as a feed additive for weaned piglets.

The invention discloses an application of N-[4-(cinnamamido) butyl] benzamide in preparation of a TLR7 ligand. The invention finds that N-[4-(cinnamamido) butyl] benzamide can be used as a ligand of TLR7, and has strong binding and interaction with swine TLR7 protein. Therefore, the N-[4-(cinnamamido) butyl] benzamide can be used for preparing a TLR7 ligand or a TLR7 immunomodulator of an animal; besides, as the TLR7 protein is conservative in evolution, the N-[4-(cinnamamido) butyl] benzamide is not limited to pigs in application and can also be applied to other species.

The present application relates to the technical field of Bacillus amyloliquefaciens, and discloses a pig-derived broad-spectrum bacteriostatic Bacillus amyloliquefaciens and application thereof; the Bacillus amyloliquefaciens provided by the present application is Bacillus amyloliquefaciens Y106-A1-3. The bacterium is isolated from the excrement of healthy pigs, and the gastrointestinal tolerance test proves that the bacterium has strong gastrointestinal tolerance; and experiments prove that the fermentation supernatant of the Bacillus amyloliquefaciens has a bacteriostatic effect and a wide bacteriostatic spectrum, and can be used for regulating the intestinal microecological balance of animals, preventing diseases and improving the growth and production performance of animals. The microbial preparation containing the Bacillus amyloliquefaciens and the related components of the fermentation liquor has very important significance for reducing the use of antibiotics, improving the quality of agricultural and livestock products and protecting the environment, and has a good application prospect.

The invention belongs to the field of biological detection, and particularly relates to an sgRNA and RPA primer group for synchronously detecting four porcine-derived viruses, a detection system and application. The sgRNA and RPA primer group for synchronously detecting the four porcine-derived viruses comprises a sgRNA and RPA primer group for detecting a porcine acute diarrhea syndrome coronavirus, a porcine rotavirus, a porcine transmissible gastroenteritis virus and a porcine epidemic diarrhea virus. The invention further provides a centrifugal micro-fluidic chip for synchronously detecting the four swine-derived viruses, the chip can be used for synchronously and highly specifically discriminating various swine-derived viruses and accurately distinguishing infection types in a single detection process, and a detection result has laboratory-level sensitivity and accuracy.

The invention belongs to the technical field of preparation of microorganisms and vaccines thereof, and relates to an F-type pasteurella multocida strain and an application of the F-type pasteurella multocida strain in preparation of vaccines, the strain is classified and named as pasteurella PmF-19 (Pasteurella sp.F-19), the strain is preserved in China Center for Type Culture Collection on October 30, 2025, and the preservation number is CCTCC NO: M 20252391. The pig pasteurella multocida provided by the invention has very strong toxicity, the vaccine prepared by inactivation of the pig pasteurella multocida has good safety, the vaccine generates high-level antibodies after immunizing mice and pigs, and the vaccine has cross protection force on F and D serotype pasteurella multocida infection in the mice, and can be used for immunizing the mice and pigs. Meanwhile, complete protection of F-type strains can be provided for pigs, and the pigs can resist attack of F-type Pm lethal dose.

The application belongs to the field of biological detection, and particularly relates to sgRNA and RPA primer group, a detection system and application for synchronous detection of four pig-derived viruses. The sgRNA and RPA primer group for synchronous detection of four pig-derived viruses comprises sgRNA and RPA primer group for detecting porcine acute diarrhea syndrome coronavirus, porcine rotavirus, porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus. The application further provides a centrifugal microfluidic chip for synchronous detection of four pig-derived viruses, which can be used to synchronously and highly specifically identify multiple pig-derived viruses and accurately distinguish infection types in a single detection process, and the detection result has both laboratory-level sensitivity and accuracy.

The invention belongs to the technical field of biology, and particularly discloses antigen peptide of swine IRF5 protein, a polyclonal antibody of the antigen peptide, a preparation method and application of the polyclonal antibody. Wherein the amino acid sequence and the nucleotide sequence of the antigen peptide are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2. A segment of antigen peptide is obtained by conducting antigen peptide prediction on pig-source IRF5 protein, the inventor synthesizes the antigen peptide through an Fmoc solid-phase method, New Zealand white rabbits are immunized with the obtained antigen peptide, the polyclonal antibody of the IRF5 protein is prepared and obtained, the antibody can effectively react with IRF5 protein in different pig tissues and pig-source cells, and the immunogenicity of the IRF5 protein is improved. And a powerful tool is provided for IRF5 protein detection and biological function research.

The invention discloses a multiple qPCR (quantitative polymerase chain reaction) detection kit for simultaneously detecting four fimbriae genes of swine pathogenic escherichia coli and application of the multiple qPCR detection kit. The kit comprises primers and a TaqMan probe, wherein the primers and the TaqMan probe are respectively used for detecting F4, F17, F18 and F41 fimbriae genes of swine pathogenic escherichia coli. Experiments prove that the kit has the advantages of low cost, high specificity, high sensitivity, rapidness and reliability in detection, simplicity in operation and the like when being used for detection. According to recent epidemiological investigation results, 951 pig anus swab samples are detected by using the method, 662 positive samples are detected, the positive rate is 69.61%, the sensitivity of the method is higher than that of conventional PCR, and the total coincidence rate of the two results is greater than 90%. The method is suitable for rapid molecular diagnosis of swine pathogenic Escherichia coli, is an effective technical means for screening the virulence gene of the swine pathogenic Escherichia coli epidemic strain, and fills the blank of multiple fluorescent PCR detection technology in the field.

The invention discloses a swine salmonella primer composition, a kit and application, the primer composition comprises a wzy154 upstream primer, a wzy154 downstream primer, a donor probe and a receptor probe, and further comprises an ST typing primer capable of amplifying aroC, dnaN, purE, hemD, thrA, hisD and sucA. The primer composition can be used for identifying the serotype of the swine salmonella, and compared with a slide agglutination test, the primer composition is simple and convenient to operate, short in time consumption and low in requirements on experience and operation level of experimenters, and has remarkable cost and flux advantages. The ST typing primer is further integrated by optimizing the primer composition, and PCR independent amplification of seven housekeeping genes is optimized into five reactions, so that the typing efficiency is remarkably improved, and epidemiological monitoring and evolution research are facilitated.

The invention provides a method for detecting five virulence factors of enterotoxigenic escherichia coli in a pig farm based on 5-plex mPCR, and belongs to the technical field of biological detection. The multiplex PCR primer group comprises a primer pair used for detecting escherichia coli STa fimbriae genes, a primer pair used for detecting escherichia coli STb fimbriae genes, a primer pair used for detecting escherichia coli LTb fimbriae genes, a primer pair used for detecting escherichia coli STX2e fimbriae genes and a primer pair used for detecting escherichia coli EAST1 fimbriae genes. The invention provides a method for detecting five virulence factors of enterotoxigenic escherichia coli in a pig farm based on 5-plex mPCR. The aim of simultaneously, accurately and efficiently detecting the five most critical virulence factors (STa, STb, LTb subunit, STX2e and EAST1) of swine ETEC is achieved.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.

The invention discloses an application of a product of Limosilbacillus reuteri LR1 (Limosilbacillus reuteri LR1) for producing gamma-aminobutyric acid (gamma-aminobutyric acid) from pigs in prevention and treatment of vomitoxin toxicity and a preparation method of the Limosilbacillus reuteri LR1. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 64522, and the preservation date is April 17, 2024. The strain is separated and purified from a pig cecum content sample, the performance of gamma-aminobutyric acid produced by fermentation of the strain is stable, and the yield of gamma-aminobutyric acid is 0.6 g / L. The strain has a good protection effect on prevention and treatment of vomitoxin toxicity, can be applied to preparation of products for prevention and treatment of animal vomitoxin toxicity and strains for fermentation production of GABA, can be applied to different fields of veterinary microecologics, feed production and the like, and has a wide application prospect.

The invention discloses a strain of pathogenic escherichia coli phage, which is an escherichia coli phage GXECL1-19 (escherichia coli phage GXECL1-19), and the preservation number of the escherichia coli phage is CCTCC (China Center for Type Culture Collection) NO: M 20242554; the invention also discloses an application of the pathogenic coliphage in preparation of drugs for preventing and treating coliphage infection diseases. The pathogenic Escherichia coli bacteriophage disclosed by the invention can be used for effectively cracking swine pathogenic Escherichia coli bacteriophage, wherein the swine pathogenic Escherichia coli bacteriophage comprises Escherichia coli phage GXPD-01 and another strain of swine escherichia coli; furthermore, the pathogenic Escherichia coli bacteriophage disclosed by the invention can continuously inhibit bacteria for a long time, is high in stability and good in safety, can be easily prepared into a preparation and the like, and has good killing, preventing and controlling effects on environments, feeds and the like polluted by the Escherichia coli bacteriophage.

The invention belongs to the technical field of biology, and particularly discloses application of an African swine fever virus host restrictive factor PML in disease-resistant breeding screening, which comprises the following steps: S1, constructing a target gene knockout porcine cell line by using a CRISPR / Cas9 system; s2, infecting the target gene knockout porcine cell line and the control group porcine cell line which is not knocked out by using an African swine fever gene-deleted strain carrying a fluorescence label; and S3, comparing the proliferation levels of viruses in the porcine cell line with the target gene knocked out and the porcine cell line of the control group without the target gene knocked out. A PML knockout cell line is constructed through a CRISPR / Cas9 technology, knockout cells and wild type cells are infected with ASFV strains carrying fluorescence labels, the virus proliferation level is compared through fluorescence intensity, qPCR and WB, it is proved that PML is a host restriction factor of ASFV, and a key target spot and a theoretical basis are provided for breeding African swine fever resistant gene editing pig breeds and developing an ASF prevention and control technology.