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Extraction method of swine-origin antibacterial peptide

An extraction method and technology of antimicrobial peptides, applied in the field of medicine and biology, can solve the problems affecting the degree of cell fragmentation, reducing the utilization rate of raw materials, and affecting the extraction of antimicrobial peptides, so as to reduce damage and increase the utilization rate

Inactive Publication Date: 2017-07-25
派生特(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, in the process of roughly extracting porcine-derived antimicrobial peptide tissue and mashing it, because it contains large tissue pieces, when the homogenate is mashed, it will cause great damage to the machine and reduce the utilization rate of raw materials; when the tissue is not mashed enough When it is sufficient, it will affect the degree of cell fragmentation during repeated freezing and thawing, and ultimately affect the extraction of antimicrobial peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting pig-derived antimicrobial peptides, comprising the following steps:

[0029] 1) Take fresh or thawed porcine small intestine, remove the fascia, fat tissue and small intestinal content on the surface of the porcine small intestine, rinse it with water, then heat-treat the pig small intestine in water at 90°C for 20 seconds, cool it quickly and grind it with a meat grinder , and then use a colloid mill to homogenize 3 times to obtain a pig small intestine homogenate;

[0030] 2) Mix the above-mentioned homogenate and water for injection evenly according to the mass ratio of 100:97.13, then divide the homogenized solution into containers without heat source and freeze it in a freezer. After the freezing is complete, take it out and place it Thaw in running water at room temperature, repeat freezing and thawing 6 times;

[0031] 3) Mix the homogenate after repeated freezing and thawing with acetic acid (analytical pure) according to the mass ratio ...

Embodiment 2

[0035] A method for extracting pig-derived antimicrobial peptides, comprising the following steps:

[0036] 1) Take fresh or thawed porcine small intestine, remove the fascia, fat tissue and small intestinal content on the surface of the porcine small intestine, rinse it with water, then heat-treat the pig small intestine in water at 80°C for 25 seconds, cool it down quickly, and grind it with a meat grinder , and then use a colloid mill to homogenize 3 times to obtain a pig small intestine homogenate;

[0037] 2) Mix the above homogenate and water for injection evenly according to the mass ratio of 100:90, then divide the homogenate into containers without heat source and freeze in the freezer, take it out after the freezing is complete, and place Thaw in running water at room temperature, repeat freezing and thawing 6 times;

[0038] 3) Mix the homogenate after repeated freezing and thawing with acetic acid (analytical pure) according to the mass ratio of 100:10, and stir a...

Embodiment 3

[0042] A method for extracting pig-derived antimicrobial peptides, comprising the following steps:

[0043] 1) Take fresh or thawed porcine small intestine, remove the fascia, adipose tissue and small intestinal content on the surface of the porcine small intestine, rinse it with water, then heat-treat the porcine small intestine in water at 100°C for 15 seconds, cool it down quickly, and grind it with a meat grinder , and then use a colloid mill to homogenize 3 times to obtain a pig small intestine homogenate;

[0044] 2) Mix the above homogenate and water for injection evenly according to the mass ratio of 100:99, then divide the homogenate into containers without heat source and freeze in the freezer, take it out after the freezing is complete, and place Thaw in running water at room temperature, repeat freezing and thawing 5 times;

[0045] 3) Mix the homogenate after repeated freezing and thawing with acetic acid (analytical pure) according to the mass ratio of 100:1, an...

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Abstract

The invention discloses an extraction method of a swine-origin antibacterial peptide. The method comprises the following steps of firstly carrying out heat treatment on pretreated small intestines of a pig in water of 80-100 DEG C for 15-25s to achieve the targets of improving the raw material utilization rate and making cell disruption fuller during repeated freezing and thawing; preparing homogenate of the small intestines of the pig, mixing the homogenate with injection water at the mass ratio of 100:(90-99) evenly, repeatedly freezing and thawing for 5-6 times, mixing the homogenate with acetic acid (analytically pure) in a mass ratio of 100:(1-10), carrying out a boiling water bath, cooling and centrifuging, collecting sediments and primary supernate; mixing the sediment with 2.87% acetic acid solution in a mass ratio of 1:(0.8-1) evenly, freezing, centrifuging and collecting secondary supernate; and merging the supernate and then carrying out tangential flow filtration and ultrafiltration in sequence, adjusting a pH value and filtering again and finally obtaining the swine-origin antibacterial peptide. According to the method, the utilization rate of the raw materials can be improved, the loss of an instrument in the tissue mashing process is reduced, meanwhile, cell disruption is more sufficient during repeated freezing and thawing, and the extraction rate of the swine-origin antibacterial peptide is improved.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a method for extracting pig-derived antimicrobial peptides. Background technique [0002] Antimicrobial peptide is an active polypeptide with strong antibacterial effect produced by organisms, and is an important component of biological innate immunity. So far, a variety of endogenous antibacterial active peptides have been found in many organisms including animals, plants and prokaryotes. Porcine antimicrobial peptides are antimicrobial peptides isolated from pigs. [0003] Because antimicrobial peptides have the advantages of broad-spectrum bactericidal effect, relatively small molecular weight, thermal stability and good water solubility, it is more important that antimicrobial peptides can act on prokaryotic cells and diseased eukaryotic cells and are not easy to cause pathogenic microorganisms to develop drug resistance. Therefore, under the seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/14C07K1/34
CPCC07K14/4723
Inventor 马绍伟罗贵峰徐磊刘友霖
Owner 派生特(福州)生物科技有限公司
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