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Enterococcus faecalis, micro-ecological preparation and application of micro-ecological preparation

A micro-ecological preparation, Enterococcus faecalis technology, applied in the field of microorganisms, can solve problems such as the reproductive system that has not been mentioned, achieve and maintain micro-ecological balance, ensure physiological functions, and improve the effect of micro-ecological balance

Active Publication Date: 2013-10-02
中山市健邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enterococcus faecalis is one of the normal flora in the intestinal tract of humans and animals. It can inhibit the growth and reproduction of some pathogenic bacteria, reduce the production of ammonia gas, and improve the body's disease resistance. It is one of the probiotics and has been studied at home and abroad. Reported, but no mention of reproductive system application

Method used

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  • Enterococcus faecalis, micro-ecological preparation and application of micro-ecological preparation
  • Enterococcus faecalis, micro-ecological preparation and application of micro-ecological preparation
  • Enterococcus faecalis, micro-ecological preparation and application of micro-ecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation of embodiment 1 porcine source Enterococcus faecalis

[0023] Bacteria were isolated from the reproductive tract mucosa and secretions of healthy and high-yielding sows in Dengfeng Breeding Farm, Guangzhou. The specific isolation steps were as follows:

[0024] Under sterile conditions, take a small amount of uterine secretions from sows, add them to sterile distilled water, fully shake for 5 minutes, and serially dilute by 10 times, starting from 10 -1 ~10 -6 Gradient, choose the appropriate gradient, take 100uL and spread it on the culture dish of the separation medium, culture it statically at 37°C and count. Pick a single colony from the culture dish of the separation medium, streak and purify for 3 generations, and carry out Gram staining test and microscopic examination. A large number of various bacteria were obtained, and after toxicity testing, screening and purification, a non-toxic strain III was finally selected, named Enterococcus faecalis f...

Embodiment 2 Embodiment 1

[0026] The characteristic research of the porcine source Enterococcus faecalis that embodiment 2 embodiment 1 isolates

[0027] 1. Physiological and biochemical characteristics

[0028] Set four repetitions of the first generation and higher generation (96-100 generations) of the strain. After testing, the results of many tests are completely consistent, that is, it is Gram-positive, does not form spores, does not produce contact enzymes and Oxidase, can produce lactic acid, O / F test fermentation, can utilize glucose, mannose, melezitose, fructose, ribose, amygdalin, trehalose, cellobiose, xylose, maltose, lactose, raffinose, Twenty kinds of carbohydrates, such as sorbose, sorbitol, melibiose, galactose, mannose, mannitol, arabinose, salicin, sodium gluconate and sucrose, can produce acid, and sodium gluconate can also produce gas, but Glucose does not produce gas; in addition, escin and rhamnose cannot be used, all negative, which preliminarily shows that there is no obvio...

Embodiment 3 Embodiment 1

[0052] Example 3 The application of the porcine-derived Enterococcus faecalis isolated in Example 1 in the prevention and treatment of reproductive disorders in female animals

[0053] The porcine-derived Enterococcus faecalis isolated in Example 1 was inoculated in the isolation medium (the inoculum size was 10-15%, and the fermentation culture time was 18-22h), the fermented culture was mixed evenly, and then 1‰-5 ‰ of oligosaccharides was formulated into a probiotics, named Gong Kangbao (Su), and the following tests were carried out:

[0054] 1. A preventive test was conducted on more than 300 postpartum sows in a pig farm of Dongguan Shichu Company with Gongkangbao (vegetarian) (amount of 10-20mL / head), and the result showed that the incidence of birth canal inflammation was reduced from 35% To about 5%, the reproductive performance of sows has been greatly improved, the average litter size reached 10.37-10.95, and the qualified number of litters reached 9.19-9.70, both of...

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Abstract

The invention discloses enterococcus faecalis, a micro-ecological preparation and an application of the micro-ecological preparation. The 16SrDNA sequence of enterococcus faecalis is shown in SEQ ID NO:1 and the collection number of enterococcus faecalis is CGMCC (China General Microbiological Culture Collection Center) No.6970. The micro-ecological preparation comprises enterococcus faecalis. Enterococcus faecalis is resistant to heat, acid, alkali and dryness, has strong resistance, more stable heredity and no toxicity and can suppress eight common pathogenic bacteria of pigs and cows. The micro-ecological preparation can promote sperm motility of pigs and cows and prolong the lives of pigs and cows, thus purifying the environments of genital tracts of female animals, improving and maintaining micro-ecological balance, ensuring normal physiological functions so that the female animals rut and ovulate on time and improving the fertility rate and litter size. The micro-ecological preparation has great scientific significance and good economic benefits when being applied to preventing various reproductive disturbance diseases of female animals.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically, the invention relates to a porcine-derived Enterococcus faecalis, a microecological preparation containing the porcine-derived Enterococcus faecalis and applications thereof. Background technique [0002] Reproductive disorders in dams, such as anoestrus, infertility, miscarriage, stillbirth, mummified fetus, small litter size, and weak litter, are common and widespread both at home and abroad, causing serious economic losses. Although the application of traditional prevention and treatment methods, such as a large number of antibacterial drugs, such as antibiotics, has received good results; however, due to long-term use, sometimes even abuse of such drugs, the curative effect does not increase but decreases, and edema and lesions of the reproductive tract appear. In addition, the emergence of drug-resistant strains that have not been noticed by people has brought s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P15/08C12R1/46
Inventor 黄引贤
Owner 中山市健邦生物科技有限公司
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