Porcine antibacterial peptide PMAP-23 variant and application thereof in preparation of feed

A PMAP-23, antimicrobial peptide technology, applied in the direction of cationic antimicrobial peptides, applications, antibacterial drugs, etc., can solve the problems of low yield of natural antimicrobial peptides, small yield of antimicrobial peptides, and high separation difficulty, and achieves improved growth performance and increased inhibition. Bacterial activity and safety, hemolysis rate reduction effect

Active Publication Date: 2021-06-25
山东中科康源生物科技有限公司 +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are very few antimicrobial peptides that are actually used in the field of feed, because the antimicrobial peptides from natural sources have certain immunogenicity due to their large molecular weight, and are cytotoxic to host cells, such as producing hemolysis to red blood cells; and Natural antimicrobial peptides usually have a small yield, high s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine antibacterial peptide PMAP-23 variant and application thereof in preparation of feed
  • Porcine antibacterial peptide PMAP-23 variant and application thereof in preparation of feed
  • Porcine antibacterial peptide PMAP-23 variant and application thereof in preparation of feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: preparation of antibacterial peptide, antibacterial activity test and bactericidal kinetic test

[0033] (1) Design and synthesis of antimicrobial peptides:

[0034] On the basis of the original porcine antimicrobial peptide PMAP-23, some amino acids of the antimicrobial peptide were modified, and the antimicrobial peptide with the following amino acid sequence was obtained:

[0035] Antimicrobial peptide A: mutate the 6th position of PMAP-23 from leucine (L) to lysine (K) to obtain antimicrobial peptide A with the amino acid sequence of SEQID No: 3;

[0036] SEQ ID NO: 3: N-RIIDLKWRVRRPQKPKFVTVWVR-C

[0037] Antimicrobial peptide B: mutate the 6th position of PMAP-23 from leucine (L) to lysine (K), and from 20th position of valine (V) to cysteine ​​(C) to obtain the amino acid sequence Antimicrobial peptide B of SEQ ID No: 4;

[0038] SEQ ID NO: 4: N-RIIDLKWRVRRPQKPKFVTCWVR-C

[0039] Antimicrobial peptide C: mutate the 6th leucine (L) of PMAP-23 to l...

Embodiment 2

[0054] Embodiment 2: acid-base stability, thermostability test

[0055] (1) Acid-base stability test

[0056] To test the effect of pH value, temperature and salt on the antibacterial activity of antimicrobial peptides, ddH was prepared with HCl and NaOH 2 O, so that the pH values ​​are 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, ddH with different pH values ​​will be prepared 2 O Dissolve antimicrobial peptides A-C and original PMAP-23, according to the method of "(3) antibacterial activity test" in Example 1, use Escherichia coli as a marker bacterium, observe the impact of different treatments on antibacterial peptide antibacterial activity, For specific results, see image 3 shown.

[0057] based on image 3 The displayed results show that the activity of the original PMAP-23 decreases when the pH exceeds 7, and its activity basically disappears when the pH reaches 13; When the pH was 12, the antimicrobial peptide C of the present invention showed an obvious decline in ac...

Embodiment 3

[0061] Embodiment 3: hemolytic activity test, feeding safety test

[0062] In order to evaluate the safety of antimicrobial peptides for animals, a hemolytic activity test and feeding safety test were carried out. The specific test plan is as follows:

[0063] (1) Hemolytic activity test

[0064] The defibrated pig blood was centrifuged at 1000 rpm for 10 min, the supernatant was discarded, and the red blood cells were repeatedly washed with PBS until the supernatant was clear. Resuspend red blood cells with 10 times the volume of PBS to prepare red blood cell suspension. The antimicrobial peptide antimicrobial peptide C and the original PMAP-23 antimicrobial peptide were serially diluted with PBS according to the doubling dilution method, and diluted to concentrations of 10240, 5120, 2560, 1280, 640, 320, 160, 80, and 40 μg / mL, respectively. Take 10 μL antimicrobial peptide solution and add it into the 96-well plate as the experimental group and control group, and take 10 μ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a porcine antibacterial peptide PMAP-23 variant and application thereof in preparation of feed. A peptide fragment of a porcine antibacterial peptide PMAP-23 is modified, so that the antibacterial activity and safety of the antibacterial peptide are improved, the antibacterial peptide variant has improved thermal stability, the porcine antibacterial peptide PMAP-23 variant is further prepared into a feed additive which can be used for feeding piglets and other animals, and is used for improving the growth performance of the animals, reducing the feed conversion ratio and improving the economic benefit of breeding; and as the variant of the natural antibacterial peptide, the antibacterial peptide variant does not bring drug resistance to fed animals, and has positive social significance.

Description

technology field; [0001] The invention belongs to the field of biotechnology, and relates to a pig-derived antimicrobial peptide PMAP-23 variant and its application, in particular to a pig-derived antimicrobial peptide PMAP-23 and its application in preparing feed. Background technique: [0002] Antibiotics are the first choice for the treatment and prevention of infectious diseases in animals. They have been used in animal production for 60-70 years. They can prevent and treat diseases caused by harmful bacteria and can promote animal growth. It has played a huge role in promoting the development of livestock and poultry and aquaculture. However, with the widespread use of antibiotics in the animal husbandry industry, some problems have gradually emerged, such as veterinary drug residues and antibiotic pollution in the environment can cause direct toxicity and allergic effects on the human body, and for example, multi-drug resistant bacteria spread rapidly, and even " Supe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/47A23K50/30A23K50/75A23K20/147A61K38/17A61P31/04
CPCC07K14/4723A23K50/30A23K50/75A23K20/147A61P31/04A61K38/00
Inventor 秦永全曹荣田徐赓赵树发张志刚聂伟宋善友刘明胜郭长城朱成升赵平朱琳
Owner 山东中科康源生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap