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The application discloses a rice gene expression and function verification integrated detection device, which comprises a box body, a liquid nitrogen freezing assembly for freezing rice is installed in the box body, a gene extraction assembly for extracting rice genes is installed in the box body, and a gene detection assembly for detecting and verifying rice genes is installed at the top of the box body; a plurality of steps such as liquid nitrogen freezing, sample grinding, nucleic acid extraction, purification and qPCR detection are integrated, full-process automatic operation is realized through a control module, manual frequent intervention is not needed, human operation errors are greatly reduced, the accuracy and repeatability of detection results are improved, a high-concentration saline solution is combined, a low-salt elution process design is adopted, automatic magnetic bead adsorption and cleaning processes are combined, interference substances such as proteins and impurities in samples are effectively removed, the nucleic acid purity can reach more than 95%, the recovery rate can reach more than 85%, and the reliability of gene expression and function verification results is ensured.