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Kadsurae Caulis-loading I/III type collagen composite scaffold

A technology of composite scaffolds and Phyllostachys chinensis, applied in the field of biomedicine, can solve the problems of secondary lesions in the donor area, unable to fully restore the healthy shape and physiological function of damaged cartilage tissue, limited donor sources, etc., and achieve good porosity and Effect of swelling ratio

Active Publication Date: 2019-02-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, surgical treatment cannot fully restore the original healthy shape and physiological function of damaged cartilage tissue, and there are shortcomings such as limited donor sources, secondary lesions at the donor site, and immune rejection.

Method used

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  • Kadsurae Caulis-loading I/III type collagen composite scaffold
  • Kadsurae Caulis-loading I/III type collagen composite scaffold
  • Kadsurae Caulis-loading I/III type collagen composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) ① Accurately weigh 0.06 g PLGA (molecular weight: 50,000, ratio: 75:25), dissolve in 1.2 mL of dichloromethane, and store in a refrigerator at 4°C for later use; ② Accurately weigh 0.5 mg of Spatholobus Spatholobus Place in 10 mL of ultrapure water, and dissolve it completely at 37°C; ③accurately weigh 0.09 g of PVA, place it in 9 mL of ultrapure water, and dissolve it completely in a water bath at 60°C; Take 600 uL of the Caulis Spatholobus solution described in step ② and slowly inject it into the PLGA solution described in step ①; will step The mixed liquid was emulsified in an ice-water bath for 2 minutes with a cell disruptor with a probe amplitude of 35% and a 3 mm conical probe (power of 200 W) to form colostrum; will step The colostrum formed in the step is immediately poured into the PVA solution, then use the steps Emulsify in an ice-water bath for 2 min in the cell disruptor to form a double emulsion; will step The double emulsion is fully ...

Embodiment 2

[0038] Change 0.8% beef tendon in step 4) Type collagen solution, 0.8% recombinant human source The mass ratio of type collagen solution was 1:2, and other steps were the same as in Example 1 to prepare composite material B. Its porosity and swelling rate can be as high as 83.9% and 40.1%, respectively.

Embodiment 3

[0040] Change 0.8% beef tendon in step 4) Type collagen solution, 0.8% recombinant human source The mass ratio of type collagen solution was 1:3, and other steps were the same as in Example 1 to prepare composite material C. Its porosity and swelling rate can be as high as 84.1% and 40.2%, respectively.

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PUM

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Abstract

The invention discloses a Kadsurae Caulis-loading I / III type collagen composite scaffold, which is prepared by using bovine source I type collagen, recombinant human source III type collagen, KadsuraeCaulis and PLGA as raw materials. The preparation process comprises: preparation of Kadsurae Caulis-loading PLGA microspheres, preparation of I / III type collagen composite scaffold, loading of Kadsurae Caulis-loading PLGA microspheres, and other steps. According to the present invention, the prepared composite scaffold integrates the characteristics of the I type collagen ribbon fiber structure and the III type collagen reticular fiber structure, further has characteristics of suitable three-dimensional network structure, good biocompatibility, no toxicity, degradability and low immunogenicity, integrates the osteoarthritis treatment effect of Kadsurae Caulis, and is the good medical material for osteoarthritis cartilage defect repair and treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a type I / type III collagen composite scaffold loaded with spaghetti spaghetti and a preparation method thereof. Background technique [0002] In recent years, the incidence of joint diseases in my country has been increasing. Articular cartilage is a very complex hyaline-like cartilage connective tissue with a smooth and shiny surface, which mainly plays the role of weight-bearing and movement in joint activities. When the cartilage is damaged, its nutrient absorption is limited and the tissue lacks blood supply, which will lead to the gradual deterioration of the condition of the injured part, and eventually develop into knee osteoarthritis (Knee osteoarthritis, KOA). Sexual pain, swelling, mobility impairment, severe knee joint deformity and even disability seriously affect the life and health of patients. Although there are many causes of KOA, the defects and degenerat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/56A61L27/58A61L27/50A61L27/54A61L27/36
CPCA61L27/24A61L27/36A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/30A61L2300/41A61L2300/602A61L2300/622
Inventor 王建华王营营黄晨光孙晓敏张其清
Owner FUZHOU UNIV
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