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Rubber tree growth-promoting rhizobacterium strain HBRM-86 and application thereof

A HBRM-86, rubber tree technology, applied in the field of plant microorganisms, can solve problems such as natural rubber yield restriction

Active Publication Date: 2016-03-09
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to screen out the bacterial strains that can be applied to the prevention and control of dead skin of rubber trees with the rhizosphere growth-promoting effect of rubber trees, so as to solve the problem of natural rubber production restriction

Method used

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  • Rubber tree growth-promoting rhizobacterium strain HBRM-86 and application thereof
  • Rubber tree growth-promoting rhizobacterium strain HBRM-86 and application thereof
  • Rubber tree growth-promoting rhizobacterium strain HBRM-86 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and purification of strain HBRM-86 and its ACC deaminase activity assay

[0029] 1.1 Sampling

[0030] The sample is the rhizosphere soil of rubber trees in Guangba Farm, Dongfang City, Hainan Province. When sampling, avoid roadsides and fertilization ditches, and select forest segments with no obvious environmental impact (including water pollution and human impact) for distribution according to the diagonal method. Put the rubber tree fine roots with the rhizosphere soil collected into a plastic bag and make a record.

[0031] 1.2 Isolation and purification of strains

[0032] 1.2.1 Sample processing

[0033] Take 5 sections of roots with a size of about 1 cm and put them into 50 mL of sterile water, soak them for 10 min, shake them slightly for 5 min, and make a suspension for later use.

[0034] 1.2.2 Isolation and purification of strains

[0035] Take 1mL of the suspension and add it to 50mL of PAF culture medium, shake it at 28°C and 200r / min...

Embodiment 2

[0046] Identification of embodiment 2 bacterial strain HBRM-86

[0047] 1. Microbiological characteristics

[0048] Observe the morphological characteristics of the HBRM-86 strain cultured in a 28°C incubator, as shown in figure 1 As shown, the biocontrol strain HBRM-86 was found to be straight rod-shaped, Gram-stained negative, with perinatal flagella, and facultative anaerobic; after culturing on TSB medium for 48 hours, the colony was nearly round, opaque and with neat and smooth edges , milky white to yellowish, raised in the middle and darker in color, thinner at the edge, smooth, moist, sticky in texture, easy to pick, with an average colony diameter of 8.51 mm and an average diameter of 6.27 mm in 24 hours.

[0049] 2. Molecular biological identification

[0050] Using the total DNA of the strain as a template, the general primer 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and the reverse primer 1492R (5'-ACGGCTACCTTGTTACGACTT-3') were used to amplify 16S rDNA. The primers were ...

Embodiment 4

[0095] The formulation of embodiment 4 growth curve

[0096] Cultivate the strain in TSB medium overnight (10-12h), inoculate it into sterile medium according to the inoculum size of 1%, and after mixing evenly, take 5mL of the mixed solution into sterile test tubes marked with time, at the optimum Shake culture at growth temperature (200r / min), select a total of 17 time points from 0 to 50h for timing sampling and measurement, use uninoculated liquid medium as blank control, and select 600nm wavelength for colorimetric measurement. The culture solution with high cell density is properly diluted with liquid medium and then measured so that its OD600 is within 0.1-0.65 (before measuring the OD value, shake the culture solution to be measured to make the cells evenly distributed).

[0097] Taking time as the abscissa and OD600 as the ordinate, draw the growth curve of the bacteria, see figure 2 .

[0098] From the growth curve of the strain HBRM-86, it can be seen that the la...

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Abstract

The invention specifically relates to enteroaerogen HBRM-86 and applicaton of the same as a rubber tree growth-promoting rhizobacterium strain, belonging to the technical field of plant microbes. According to the invention, ACC deaminase is used as a main index; the enteroaerogen HBRM-86 is obtained through elaborate screening and preserved in China Center for Type Culture Collection; and experimental results prove that the screened enteroaerogen HBRM-86 can effectively function as a growth-promoting rhizobacterium strain, benefits growth of the seedlings of a rubber tree and is favorable for increasing the output of natural rubber.

Description

technical field [0001] The invention belongs to the technical field of plant microorganisms, and in particular relates to Enterobacter aerogenes HBRM-86 and its application as a rubber tree rhizosphere growth-promoting bacterial strain. Background technique [0002] Natural rubber is a natural polymer compound with polyisoprene as the main component, and its molecular formula is (C 5 h 8 ) n , 91-94% of its ingredients are rubber hydrocarbons (polyisoprene), and the rest are non-rubber substances such as proteins, fatty acids, ash, and sugars. Usually what we call natural rubber refers to the elastic solid made of natural rubber latex collected from Hevea brasiliensis through coagulation, drying and other processing procedures. Natural rubber is the most widely used general-purpose rubber. At present, natural rubber is one of the four major industrial raw materials in my country alongside steel, petroleum, and coal, and the industrial demand is huge. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCA01G7/06C12N1/20C12N1/205C12R2001/01
Inventor 孙亮胡义钰廉法钦王真辉袁坤谢贵水安锋
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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