Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof

A cellulose degradation and bacteria technology, applied in the application, bacteria, preparation of organic fertilizers, etc., can solve the problems of refractory utilization and inability to decompose, and achieve the effect of rapid reproduction

Active Publication Date: 2013-12-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology cannot decompose the pectin and hemicellulose components in the straw; in addition, the bacillus is also difficult to degrade and utilize insoluble inorganic phosphorus salts

Method used

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  • Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof
  • Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof
  • Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Isolation, purification and preservation of strains

[0030] The rotten straw and soil of the paddy field of Pujiang Base of Jiaotong University, Pujiang Town, Minhang District, Shanghai were taken as samples. After different samples were ground and mixed, 10 g was taken, placed in 100 mL enrichment medium, and incubated at 30°C and 150 rpm. Every 5 days, 10% of the inoculum was transferred to another enriched medium, and the acclimatization was repeated 4 times. The enrichment medium adopts the medium with CMC-Na as the sole carbon source.

[0031] Take 100 μL of the enrichment solution and spread it on the CMC-Congo red medium plate, then culture it upside down in a constant temperature and humidity incubator at 32°C, and observe after 3 days. As a result, a transparent hydrolysis circle appears on the plate, such as figure 1 . Pick a small amount of bacteria and streak on LB solid medium until a single colony is obtained, such as figure 2 As shown, the obtained...

Embodiment 2

[0033] Identification of strains

[0034] Combining bacterial Biolog GP2 and 16S rDNA V3 ( Figure 4 ) region sequence alignment, and identify the bacteria Bacillus, the taxonomic species named Bacillus pumilus (Bacillus pumilus).

[0035] The Bacillus pumilus was named JSD-12, and it was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC) on July 15, 2013. Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Scientific Microbiology, deposit number is CGMCC No.7923.

Embodiment 3

[0037] Determination of Optimal Growth Curve

[0038] After measurement, it was determined that the most suitable medium for the bacteria was LB liquid medium, and the pH was adjusted to 7.0. The activated bacteria were inoculated into the LB liquid medium, and the inoculation amount was 1.0% of the volume of the medium, and cultured on a shaker at 32°C and 150rpm. When the incubation time is 1, 2, 4, 6, 8, 10, 12, 14, 18, 22, 26, 30, 34, 38 hours, measure the OD of the bacterial solution 600 value, draw a curve as Figure 5 shown.

[0039] The components of the LB liquid medium are: 10 g of peptone, 5 g of yeast extract, 10 g of NaCl, 1000 mL of distilled water, and pH 7.0.

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Abstract

The invention discloses a cellulose-degrading bacterium with phosphate-dissolving capability and application thereof and relates to the technical field of biological environmental protection. The cellulose-degrading bacterium JSD-12 is bacillus pumilus (Bacillus pumilus), has a preservation number of CGMCC No.7923 and is preserved on July 15, 2013. The bacterium not only has strong degradation capability to cellulose, but also has certain capability of decomposing hemicellulose and pectin components in the straw. The bacterium can rapidly breed, can tolerate high temperature which is over 60 DEG C, and can be prepared into added microbial inoculums for high-temperature composting. In addition, the bacterium also has the phosphate-dissolving capability, and can be used for production of bacterial manure.

Description

technical field [0001] The invention relates to a method in the technical field of biological environmental protection, in particular to a cellulose-degrading bacterium capable of dissolving phosphorus and its application. Background technique [0002] Cellulose is the main product of plant photosynthesis and is the most abundant renewable resource in nature. The annual output of crop straw in the world is about 2.9 billion tons, while the annual output of straw in my country can reach more than 700 million tons. The content of cellulose in crops is very high, up to 40%-50%. Since cellulose is a high-molecular polymer containing a large number of high-energy hydrogen bonds, it is difficult to degrade and hydrolyze, so its utilization rate is very low. According to statistics, about 89% of the straw has not been effectively used, and most of them are discarded or even burned, which not only causes waste of resources but also pollutes the environment. [0003] Under the back...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C05F11/08C12R1/01
CPCY02W30/40
Inventor 周培冯海玮支月娥卫星罗艳青毛亮
Owner SHANGHAI JIAO TONG UNIV
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