Method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil

A technology of cadmium-contaminated soil and Aspergillus aculeatus, which is applied in the restoration of contaminated soil, etc., can solve the problems of low efficiency of remediation of heavy metals, short activity duration, and low survival rate of microorganisms, so as to improve the transfer coefficient and remediation efficiency, improve Effects of translocation and accumulation, good environmental benefits

Inactive Publication Date: 2016-02-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] ① Microorganisms have poor adaptability to different soil types and soil microenvironments;
[0013] ② The poor compatibility between microorgani...

Method used

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  • Method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil
  • Method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil
  • Method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil

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Experimental program
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Effect test

preparation example Construction

[0044] A. Preparation of microbial remediation matrix:

[0045] The cadmium-resistant fungus Aspergillus aculeatus isolated, purified and identified from the rhizosphere soil of the dominant species on the heavy metal-polluted soil was propagated with Martin's liquid medium, and then the colony of Aspergillus aculeatus cultured in 15g liquid medium was mixed with 1kg after high temperature and high pressure The proportion of sterilized and crushed crop straw with a water content of 40%, inoculated the cadmium-resistant fungus Aspergillus aculeatus colony into the straw, adjusted the pH value to 6.0, and cultured at 28°C for 2 to 3 days for a large number of fungi to multiply, and prepared Form cadmium-contaminated soil microbial remediation matrix;

[0046] B. Application of microbial remediation matrix:

[0047] Add the prepared microbial remediation matrix to the surface layer of cadmium-contaminated soil in an amount of 1 kg / square meter of soil area, and at the same time ...

Embodiment 1

[0061] 1. Embodiment 1: greenhouse pot test

[0062] 1) Dry and crush the surface soil of the field, pass through a 2mm sieve, sterilize under high temperature and high pressure for 30 minutes, add cadmium content at 2 mg / kg dry weight of soil and place it in a closed environment for 2 months to obtain cadmium-contaminated soil;

[0063] 2) Add 120g of the microbial remediation matrix of Aspergillus aculeatus and 6g of compound fertilizer per kilogram to the obtained cadmium-contaminated soil, mix well and put it into a plastic pot with a height of 25cm, an upper diameter of 18cm and a lower diameter of 15cm, to add untreated soil. The straw inoculated with Aspergillus aculeatus was used as the control;

[0064] 3) Sow 30 tall fescue seeds evenly in each plastic pot, then cover with a layer of fine sand with a thickness of about 1cm, place it in a glass greenhouse for cultivation, water it appropriately every day, and keep the soil moist to facilitate the seeds Germinate; whe...

Embodiment 2

[0067] 2. Embodiment 2: Field natural environment cultivation test

[0068] 1) Application of cadmium-contaminated soil microbial remediation matrix

[0069] Add the prepared microbial remediation matrix to the surface layer of cadmium-contaminated field soil in an amount of 1 kg / square meter of soil area, and at the same time apply 20-30 kg / mu of ternary compound fertilizer with a ratio of nitrogen: phosphorus: potassium of 24:6:10, Rotary plowing on the 0-25cm tillage layer on the soil surface to mix the soil with microbial remediation matrix and compound fertilizer to level the soil;

[0070] 2) Establishment of tall fescue lawn on cadmium-contaminated soil

[0071] Cover the soil with a layer of fine sand with a thickness of about 2-3cm on the soil that has been applied with microbial remediation matrix and compound fertilizer and leveled, and spray herbicide before emergence at the same time; use the method of broadcasting, at a temperature of about 18-25°C When sowing ...

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Abstract

The invention discloses a method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil, and relates to the technical field of plant-microorganism combined ecological remediation. The method mainly comprises the steps that firstly, cadmium-resisting fungus aspergillus aculeatus separated, purified and identified out of rhizosphere soil of dominant species in heavy metal contaminated soil is bred and inoculated into crushed crop straw to be prepared into a microbial remediation matrix, and the microbial remediation matrix is applied into cadmium contaminated soil; secondly, a festuca arundinacea lawn is planted in a sowing manner; thirdly, watering of a citric acid aqueous solution of a proper concentration and a proper quantity is carried out in batches; and fourthly, the rhizosphere microenvironment of festuca arundinacea is adjusted and controlled through citric acid to promote the interaction of cadmium-resisting fungus aspergillus aculeatus and a root system of festuca arundinacea so as to improve cadmium remediation efficiency. The problem of adaptability of screened-out microorganisms to the soil environment is solved; transferring of cadmium towards ground parts of plants is promoted, and the cadmium remediation efficiency is improved; and good environmental benefits, economic benefits and social benefits are achieved.

Description

technical field [0001] The invention relates to the technical field of plant-microbe combined ecological restoration, in particular to a method for promoting tall fescue to restore cadmium-contaminated soil by using the cadmium-resistant fungus Aspergillus aculeatus. Background technique [0002] Tall fescue (Latin name: Festuca arundinacea) is native to Europe and is a perennial herbaceous plant of the genus Fescue in the Poaceae family. It is widely distributed in the subtropical, temperate and warm temperate humid transition zones. Southwest and Xinjiang, etc., grow on roadsides, hillsides and forests, and are suitable for a variety of soil and climate conditions. Tall fescue is a cluster type with well-developed fibrous roots, wide adaptability, strong cold resistance, good heat and drought resistance, strong trampling resistance, strong disease resistance, and low pruning resistance. As a lawn, it has a good ornamental effect and a long green period. It is widely used ...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 胡龙兴杨知建向佐湘刘明烯曾宁波
Owner HUNAN AGRICULTURAL UNIV
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