Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16

A HBRM-16, rubber tree technology, applied in the field of plant microorganisms, can solve the problems of natural rubber production constraints and other issues

Inactive Publication Date: 2016-03-16
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to screen out the bacterial strains that can be applied to the prevention and

Method used

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  • Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16
  • Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16
  • Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and purification of strain HBRM-16 and its ACC deaminase activity assay

[0030] 1.1 Sampling

[0031] The sample is the rhizosphere soil of rubber trees in Guangba Farm, Dongfang City, Hainan Province. When sampling, avoid roadsides and fertilization ditches, and select forest segments with no obvious environmental impact (including water pollution and human impact) for distribution according to the diagonal method. Put the rubber tree fine roots with the rhizosphere soil collected into a plastic bag and make a record.

[0032] 1.2 Isolation and purification of strains

[0033] 1.2.1 Sample processing

[0034] Take 5 sections of roots with a size of about 1 cm and put them into 50 mL of sterile water, soak them for 10 min, shake them slightly for 5 min, and make a suspension for later use.

[0035] 1.2.2 Isolation and purification of strains

[0036] Take 1mL of the suspension and add it to 50mL of PAF culture medium, shake it at 28°C and 200r / min...

Embodiment 2

[0047] Identification of embodiment 2 bacterial strain HBRM-16

[0048] 1. Microbiological characteristics

[0049] Observe the morphological characteristics of the HBRM-16 strain cultured in a 28°C incubator, as shown in figure 1 As shown, the HBRM-16 strain was found to be short-spherical brevibacteria, Gram-stained negative, with perinatal flagella, motility, no capsule, and no spores; after culturing on TSB medium for 48 hours, the colonies were nearly round, opaque and bordered Neat, producing red water-soluble pigment, the middle red is darker and the edge is lighter, the surface is smooth, the texture is sticky, easy to pick, the average diameter of the colony is 8.90mm, and the average diameter of 24h is 6.80mm.

[0050] 2. Molecular biological identification

[0051] Using the total DNA of the strain as a template, the general primer 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and the reverse primer 1492R (5'-ACGGCTACCTTGTTACGACTT-3') were used to amplify 16S rDNA. The primer...

Embodiment 4

[0095] The formulation of embodiment 4 growth curve

[0096] Cultivate the strain in TSB medium overnight (10-12h), inoculate it into sterile medium according to the inoculum size of 1%, and after mixing evenly, take 5mL of the mixed solution into sterile test tubes marked with time, at the optimum Shake culture at growth temperature (200r / min), select a total of 17 time points from 0 to 50h for timing sampling and measurement, use uninoculated liquid medium as blank control, and select 600nm wavelength for colorimetric measurement. The culture solution with high cell density is properly diluted with liquid medium and then measured so that its OD600 is within 0.1-0.65 (before measuring the OD value, shake the culture solution to be measured to make the cells evenly distributed).

[0097] Taking time as the abscissa and OD600 as the ordinate, draw the growth curve of the bacteria, see figure 2 .

[0098] From the growth curve of the strain HBRM-16, it can be seen that the la...

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Abstract

The invention belongs to the technical field of plant microbes, and in particular relates to serratia marcescens HBRM-16 and an application of the serratia marcescens HBRM-16 as a bacterial strain for promoting the growth of the roots of a rubber tree. According to the invention, ACC deaminase is taken as the main index, a serratia marcescens HBRM-16 strain is obtained through elaborate selection, and the serratia marcescens HBRM-16 strain is preserved in the China Center for Type Culture Collection (CCTCC); the experiment proves that the selected serratia marcescens HBRM-16 can be effectively taken as the strain for promoting the root growth, so that the growth of rubber tree seedlings is promoted, and the yield of natural rubber is improved.

Description

technical field [0001] The invention belongs to the technical field of plant microorganisms, and in particular relates to Serratia marcescens HBRM-16 (Serratia marcescens subsp. Marcescens HBRM-16) and its application as a rubber tree rhizosphere growth-promoting bacterial strain. Background technique [0002] Natural rubber is a natural polymer compound with polyisoprene as the main component, and its molecular formula is (C 5 h 8 ) n , 91-94% of its ingredients are rubber hydrocarbons (polyisoprene), and the rest are non-rubber substances such as proteins, fatty acids, ash, and sugars. Usually what we call natural rubber refers to the elastic solid made of natural rubber latex collected from Hevea brasiliensis through coagulation, drying and other processing procedures. Natural rubber is the most widely used general-purpose rubber. At present, natural rubber is one of the four major industrial raw materials in my country alongside steel, petroleum, and coal, and the ind...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P21/00C05F11/08C12R1/43
Inventor 孙亮胡义钰廉法钦王真辉袁坤谢贵水安锋
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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