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Method and equipment for improving dissolution of cellular content

A content and cell technology, applied in biochemical equipment and methods, plant cells, microorganisms dissolution, etc., can solve the problems of small quantity and strength, and achieve the effect of flexibility

Inactive Publication Date: 2009-03-11
初景涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The homogenizer that is often used in production will also produce cavitation when it is working, but the quantity and strength it produces are very small. The homogenizer mainly uses the principle of mechanical shear force to work

Method used

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  • Method and equipment for improving dissolution of cellular content
  • Method and equipment for improving dissolution of cellular content

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Example 1: Ginkgo biloba leaves are first crushed to particles below 5 mm, mixed with 50% ethanol at a weight ratio of 1:20, 500 kg of the mixed material is taken, put into a mixing tank, and the operating temperature is 26°C, and sent to In a cavitation generator with a processing liquid volume of 10 cubic meters per hour, the reflux ratio is 50%, and the total flavonoid yield is 10.39% from the effluent sampling analysis. Under the same conditions, the conventional process reflux extraction method is used twice, each time for 3 hours, and its total flavonoid yield is only 5.8%, while the viscosity of the mixture system is 16% lower than the conventional process, and the average particle size observed by a microscope is 3 to 5 microns. And evenly distributed.

example 2

[0017] Example 2: first crush fresh tea leaves to particles below 5 mm, mix them with water at a weight ratio of 1:10, take 500 kg of the mixed material, put it into a mixing tank, operate at a temperature of 25°C, and send it to be processed every hour In a cavitation generator with a liquid volume of 10 cubic meters, the reflux ratio is 10%. From the effluent sampling analysis, the tea polyphenol extraction rate is 28.5%. Under the same conditions, the conventional process is boiled for 30 minutes, and the extraction rate of tea polyphenols is 15.8%. The average particle size observed by a microscope is 3-5 microns, and the distribution is uniform.

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Abstract

The invention discloses a method for substantially increasing the dissolution of cell contents in animals, plants, and microbes. The method comprises the following steps: pre-grinding, soaking mixing, cavitation breaking, and backflow circulation, wherein the step of cavitation braking is to use a specially-designed machine to perform cavitation treatment to suspension liquid; and the machine, through a mechanical rotation mode, produces a great number of bubble clusters in the suspension liquid passing through the machine to generate strong cavitation impact force which can smash particles in the suspension liquid, accelerate the dissolution, homogenize further, and lower the system viscosity, so that cell walls or cell membranes are ruptured in order to promote the cell contents to flow out more easily, thereby substantially improving the quantity of the dissolution and the dissolution efficiency. At the same time, the invention discloses a device for initiating the cavitation phenomenon.

Description

technical field [0001] The invention relates to the field of biological substance extraction, which is a method and a process for generating cavitation phenomenon by mechanical means, thereby promoting cell wall breaking and bursting so that contents in the cells flow out. Background technique [0002] Organisms are composed of cells, and cells are composed of cell membrane, cytoplasm, and nucleus, and plant cells also have a hard cell wall. At present, humans mainly use the substances in the cell membrane, such as the active ingredients of Chinese medicinal materials, extracts from natural products, microbial fermentation, etc., all use the contents of the cells, but the complete structure of the cells has greatly hindered this. Now commonly used methods for extracting cell contents include decoction, thermal reflux, ultrasonic extraction, supercritical CO 2 , cellulase method, mechanical crushing method, solvent dissolution and other methods, all of which are based on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/04C12N1/06C12M1/00
Inventor 初景涛
Owner 初景涛
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