The invention discloses a purifying agent for treating tortoise breeding wastewater. The invention also discloses a preparation method of the purifying agent for treating turtle breeding wastewater, which comprises the following steps: adding sepiolite into water, standing, adding liquid paraffin, heating, keeping the temperature, continuously stirring in the heating and keeping process, adding into an oxalic acid solution, oscillating, carrying out suction filtration, and washing to neutrality to obtain acid-etched sepiolite fibers; adding sodium carboxymethyl cellulose into an ethanol aqueous solution, uniformly mixing, adding hydrochloric acid, stirring, adding the acid-etched sepiolite fibers, heating, stirring, carrying out a hydrothermal reaction, cooling, washing, drying, adding theproduct into a composite bacteria solution, standing, and filtering to obtain a sepiolite compound; and adding chitosan into water, continuously stirring, adding acetic acid, continuously stirring, standing overnight at room temperature, adding the sepiolite compound, uniformly mixing, dropwise adding the mixture into a curing liquid, heating, and carrying out heat preservation to obtain the purifying agent.