Catharanthus roseus total alkaloids separation method in Catharanthus roseus extract

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely Catharanthus roseus, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the Catharanthus roseus; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; checking eluent until no alkaloid exists; collecting the eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and performing concentrated drying to obtain the Catharanthus roseus total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the Catharanthus roseus total alkaloid from the extracting solution of the Catharanthus roseus.

Description

technical field [0001] The invention relates to the extraction and separation of effective components of traditional Chinese medicine, in particular to a method for extracting and separating total alkaloids of periwinkle from traditional Chinese medicine periwinkle extract, which belongs to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Periwinkle is the whole plant of Catharanthus roseus (L.) G.Don, a plant of the family Apocynaceae, also known as Ririxin, Yanlaihong, etc. In addition, there is a variant of this plant, Catharanthus roseus (L.) G .Donvar.albus (Sweet) G.Don, yellow periwinkle G.roseus (L.) G.Don var.flvus (Tsiang) Metcalr, also equally used as medicine. Periwinkle is a commonly used folk Chinese herbal medicine. It is cool in natu...

AI Technical Summary

Problems solved by technology

Although this method can improve the purity of alkaloids and has a certain degree of progress, there are still serious defects: the test found that the efficiency of alkalizing the resin with ammonia water was low, resulting in a low yield; the free periwinkle under alkaline conditions Alkaloids need to have good solubility in organic solvents, but the use of organic solvents will bring residues of resin monomer components such as styrene, divinylbenzene, and organic solvents into the eluent, which will affect the safety of the preparation; During the process, the resin needs to be poured out and dried, then alkalized with ammonia water, and then refluxed with various organic solvents for extraction. The steps are cumbersome, and pipeline production cannot be realized; at the same time, organic solvents such as ether, chloroform, and ethanol are used in the process. It is required to increase the steps of organic solvent treatment such as ethanol in the pre-treatment of the resin, and the organic solvent must be recovered later, which increases the process steps and increases the production cost