Method for improving transplanting survival rate of catharanthus roseus tissue culture seeding

A technology of transplanting survival rate and periwinkle, which is applied in the direction of botany equipment and methods, cultivation, soilless cultivation, etc., can solve the problems that the culture medium cannot be completely removed, the root system of the plant is rotten, and the transplanting survival rate is low, and achieves Improve the survival rate of transplanting, enhance the antibacterial ability, and the effect of simple and convenient operation

Inactive Publication Date: 2013-05-01
NINGBO UNIV
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after the periwinkle test-tube seedlings are transplanted into the soil, they are easily infected with bacteria, causing the roots to rot and die. The transplanted survival rate of the periwinkle test-tube seedlings is extremely low, only between 20% and 30%. The main reason is the regeneration of periwinkle The root system of the plant cannot completely remove the medium adsorbed on the

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0017] A kind of method of improving the transplanting survival rate of periwinkle tissue culture seedling of the present invention, specifically comprises the following steps:

[0018] (1) Seedling hardening: Select aseptic test-tube seedlings of periwinkle that grow normally and robustly. Before transplanting, open the cap of the culture bottle, add an appropriate amount of distilled water to the culture bottle to immerse the roots (it is better to immerse the roots), and continue to immerse in the culture bottle. Cultivate in a light incubator for 3-4 days, the culture conditions are 2000 Lx, 25°C, and the daily light cycle is 8 hours of light and 16 hours of darkness;

[0019] (2) Hydroponics: Carefully take the hardened periwinkle sterile test-tube seedlings out of the culture bottle with tweezers, rinse the medium on the root system with running water, and put them into a test tube or a petri dish filled with hydroponic nutrient solution , put it in the light incubator t...

Embodiment 2

[0024] Same as Example 1, the difference is that: the culture condition of the light incubator is 1500 Lx, 23° C., and the daily light cycle is 12 hours of light and 12 hours of darkness. Among the 30 regenerated periwinkle plants grown in hydroponic cultivation, a total of 25 survived, and the transplanting survival rate was 83.33%.

Embodiment 3

[0026] Same as Example 1, the difference is that: the culture condition of the light incubator is 2500 Lx, 27°C, and the daily light cycle is 10 hours of light and 14 hours of darkness. Among the 30 regenerated periwinkle plants grown in hydroponic cultivation, a total of 25 survived, and the transplanting survival rate was 83.33%.

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Abstract

The invention discloses a method for improving the transplanting survival rate of a catharanthus roseus tissue culture seeding. The method is characterized by comprising the following steps of: (1) selecting strongly and regularly grown catharanthus roseus aseptic test-tube seedling, opening a cover of a culture flask, adding the suitable amount of distilled water to the culture flask to immerse the root of the catharanthus roseus, and putting the catharanthus roseus seedling in an illumination incubator to culture for 3-4d to finish a seedling hardening operation; (2) taking out the catharanthus roseus aseptic test-tube seedling from the culture flask by a pair of tweezers, flushing a culture medium of the root of the catharanthus roseus aseptic test-tube seedling cleanly by running water, putting the culture medium in a test tube or a culture dish containing a water-culture nutrient solution, putting the test tube or the culture dish in the illumination incubator to continue the seedling hardening operation, and finishing the water culture when a new water root is grown up from the root of the catharanthus roseus plant after the catharanthus roseus plant is cultured for 2-7d; and (3) transplanting the newly grown catharanthus roseus water-culture plant in a transplanting medium containing vermiculite, perlite and turfy to finish the transplanting of the catharanthus roseus tissue culture seeding. The method for improving the transplanting survival rate of the catharanthus roseus tissue culture seeding disclosed by the invention has the advantages of reinforcing the antibacterial ability of the catharanthus roseus tissue culture seeding and remarkably improving the transplanting survival rate of the catharanthus roseus tissue culture seeding.

Description

technical field [0001] The invention relates to a method for transplanting periwinkle tissue cultured seedlings, in particular to a method for improving the transplanted survival rate of periwinkle tissue cultured seedlings. Background technique [0002] Periwinkle( Catharanthus roseus L.) is an annual herbaceous plant belonging to the family Apocynaceae, which can produce more than 100 anticancer terpenoid indole alkaloids (Terpenoid Indole Alkaloids, TIAs) with high medicinal value. Such as Vinblastine, Vincristine, Ajmalicine, Reserpine, Vindoline, and Catharanthine. Vinblastine is mainly used clinically to treat Hodgkin's disease and choriocarcinoma, and has a very good therapeutic effect on breast cancer. Therefore, periwinkle is considered to be a medicinal plant with high development and application value. [0003] At present, the anti-cancer alkaloids of vinca are mainly extracted from the aerial parts of vinca plants, but the content of these drugs in vinca is e...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G31/00
Inventor 龚一富潘益芳李君王何瑜袁泉卢鹏
Owner NINGBO UNIV
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