Method for improving transplanting survival rate of catharanthus roseus tissue culture seeding

Abstract

The invention discloses a method for improving the transplanting survival rate of a catharanthus roseus tissue culture seeding. The method is characterized by comprising the following steps of: (1) selecting strongly and regularly grown catharanthus roseus aseptic test-tube seedling, opening a cover of a culture flask, adding the suitable amount of distilled water to the culture flask to immerse the root of the catharanthus roseus, and putting the catharanthus roseus seedling in an illumination incubator to culture for 3-4d to finish a seedling hardening operation; (2) taking out the catharanthus roseus aseptic test-tube seedling from the culture flask by a pair of tweezers, flushing a culture medium of the root of the catharanthus roseus aseptic test-tube seedling cleanly by running water, putting the culture medium in a test tube or a culture dish containing a water-culture nutrient solution, putting the test tube or the culture dish in the illumination incubator to continue the seedling hardening operation, and finishing the water culture when a new water root is grown up from the root of the catharanthus roseus plant after the catharanthus roseus plant is cultured for 2-7d; and (3) transplanting the newly grown catharanthus roseus water-culture plant in a transplanting medium containing vermiculite, perlite and turfy to finish the transplanting of the catharanthus roseus tissue culture seeding. The method for improving the transplanting survival rate of the catharanthus roseus tissue culture seeding disclosed by the invention has the advantages of reinforcing the antibacterial ability of the catharanthus roseus tissue culture seeding and remarkably improving the transplanting survival rate of the catharanthus roseus tissue culture seeding.

Description

technical field [0001] The invention relates to a method for transplanting periwinkle tissue cultured seedlings, in particular to a method for improving the transplanted survival rate of periwinkle tissue cultured seedlings. Background technique [0002] Periwinkle( Catharanthus roseus L.) is an annual herbaceous plant belonging to the family Apocynaceae, which can produce more than 100 anticancer terpenoid indole alkaloids (Terpenoid Indole Alkaloids, TIAs) with high medicinal value. Such as Vinblastine, Vincristine, Ajmalicine, Reserpine, Vindoline, and Catharanthine. Vinblastine is mainly used clinically to treat Hodgkin's disease and choriocarcinoma, and has a very good therapeutic effect on breast cancer. Therefore, periwinkle is considered to be a medicinal plant with high development and application value. [0003] At present, the anti-cancer alkaloids of vinca are mainly extracted from the aerial parts of vinca plants, but the content of these drugs in vinca is e...

AI Technical Summary

Problems solved by technology

However, after the periwinkle test-tube seedlings are transplanted into the soil, they are easily infected with bacteria, causing the roots to rot and die. The transplanted survival rate of the periwinkle test-tube seedlings is extremely low, only between 20% and 30%. The main reason is the regeneration of periwinkle The root system of the plant cannot completely remove the medium adsorbed on the root system during the cleaning process. After transplanting to the soil, once bacteria and fungi come into contact with the medium on the root system of the plant, the nutrients in the medium will lead to the growth of bacteria and fungi. A large number of reproduction and growth will eventually cause the root system of the plant to rot, unable to fully absorb nutrients, and cause the plant to die