Inductive extraction method for alkaloid active ingredients of catharanthus roseus
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A technology of vinca alkaloids and active ingredients, which is applied in the directions of plant/algae/fungus/moss ingredients, medical preparations containing active ingredients, drug combinations, etc., to achieve the effects of low production cost, low energy consumption and high extraction rate
Inactive Publication Date: 2011-04-20
NORTHEAST FORESTRY UNIVERSITY +1
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Problems solved by technology
However, due to the barrier effect of the cell wall, the extraction efficiency of the active ingredients of Chinese herbal medicines has a certain limit.
Method used
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example 1
[0020] The concentration of cellulase was prepared at 0.3 mg / mL and the pH was adjusted between 5.0 and 6.0. Weigh 200.0g of fresh periwinkle, add enzyme solution at a solid-liquid ratio of 1:5, homogenate and pulverize for 1min, put the homogenate in a constant temperature shaker at 45°C, rotate at 90rpm, shake for 24h, and filter with suction. Add 80% ethanol solution to the solid at a solid-to-liquid ratio of 1:10 for negative pressure cavitation extraction twice, each time for 25 minutes, combine the extracts, concentrate, add 15 mL of water to the concentrate, remove impurities with petroleum ether three times, and the water phase Extracted 4 times with ethyl acetate, combined the ethyl acetate layers, concentrated to dryness, obtained 0.65g of solid matter, constant volume with methanol, HPLC detection, the content of the obtained solid matter sindolin, vinblastine, vinblastine and vincristine They were 15.46%, 7.93%, 3.81%, and 1.12%, which were 17.5%, 30.6%, 58.6%, and...
example 2
[0023] Prepare cellulase β-glucosidase concentration of 0.5mg / mL, β-glucosidase concentration of 0.5mg / mL enzyme solution, adjust the pH between 4.5-5.5. Weigh 200.0g of fresh periwinkle, add enzyme solution at a solid-liquid ratio of 1:8, homogenate and pulverize for 1.5min, put the homogenate in a constant temperature shaker at 40°C, rotate at 100rpm, oscillate for 18h, and filter with suction , adding 70% methanol solution to the solid with a solid-to-liquid ratio of 1:15, negative pressure cavitation extraction 3 times, 30min each time, combining the extracts, concentrating, adding 15mL water to the concentrate, removing impurities with petroleum ether 3 times, water The phase was extracted 3 times with dichloromethane, and the combined dichloromethane layers were concentrated to dryness to obtain 0.73 g of a solid, constant to volume with methanol, and detected by HPLC. The contents are 16.20%, 8.07%, 4.76%, 1.43% respectively, which are 21.4%, 33.6%, 72.5%, 70.8% higher ...
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Abstract
The invention relates to an extraction technology for the active ingredients of a Chinese herbal medicine catharanthus roseus, and aims at providing an efficient enzyme induction extraction method for economically and quickly obtaining a catharanthus roseus extract which is rich in four alkaloid active ingredients such as vinblastine, vincristine, catharanthine and vindoline. In allusion to the defects of long extraction time, high extraction temperature, low extraction rate and the like of the traditional extraction method, the inductive extraction method in the invention can obtain the catharanthus roseus extract which is rich in four alkaloid active ingredients by adopting the technologies such as homogenate enzyme inductive extraction, negative-pressure cavitation extraction, solvent impurity removal, enrichment refining and the like; the results show that the contents of the four alkaloid active ingredients such as vinblastine, vincristine, catharanthine and vindoline in the extract can respectively achieve 11.30-17.56%, 6.52-10.79%, 3.10-5.86% and 0.98-1.72%, which are 10-80% higher than the traditional method. The method has high extraction rate, simple equipment and safe operation, dispenses with high-temperature heating, and is suitable for the industrialized production, therefore, novel method and means are provided for the modernization of the extraction of the traditional Chinese medicine.
Description
technical field [0001] The invention relates to an inductive extraction method for obtaining vinca alkaloid extracts of vinblastine, vincristine, vinblastine and pendolin, belonging to the field of traditional Chinese medicine chemistry. Background technique [0002] Vinca is an important medicinal plant of the genus Vinca in the family Apocynaceae. It contains a variety of biologically active alkaloids, among which vinblastine and vincristine are effective in treating Hodgkin's disease and children's acute leukemia. It is one of the most widely used natural anti-tumor plant extracts in the world at present. It has great potential for development in the international market and has a very broad market prospect. my country is rich in periwinkle resources, so it is urgent to develop the production process of anti-tumor plant extracts with my country's independent intellectual property rights to develop the national economy. [0003] Traditional extraction methods such as deco...
Claims
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