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Culture method capable of improving content of vinblastine in catharanthus roseus plant body

A cultivation method, the technology of periwinkle, is applied in the cultivation field of increasing the vinblastine content in the periwinkle plant body, and can solve the problems that vinblastine cannot be produced on a large scale, cannot be synthesized, and is not suitable for production, so as to save raw materials, The effect of increasing unit output and simple and easy operation

Inactive Publication Date: 2017-07-07
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, vinblastine is mainly extracted from natural plants. Although vinblastine and its derivatives can be obtained by chemical synthesis and semi-synthetic methods, there are also studies on obtaining vinblastine through tissue culture, but they are not ideal and are not suitable for production.
The method of tissue culture cannot synthesize Vindolin, one of the precursors of Vindolin, probably because the tissue culture system cannot produce a certain enzyme required for the synthesis of Vindolin, so that tissue culture cannot produce Vindolin on a large scale

Method used

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  • Culture method capable of improving content of vinblastine in catharanthus roseus plant body

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] H with a concentration of 10mmol / L 2 o 2 The solution was evenly sprayed on the aerial part of the periwinkle seedlings whose seedling age was 3 months. After cultivating for 2 days, the branches and leaves of the periwinkle seedlings were harvested, and after drying, vinblastine was extracted and detected by high performance liquid phase method. The vinblastine content was 25.4μg / g DW, which is about 20μg / g DW of vinblastine in untreated vinca, which is an increase, but the content increase is not significant.

Embodiment 2

[0022] H with a concentration of 100mmol / L 2 o 2 The solution was evenly sprayed on the aerial part of the periwinkle seedlings whose seedling age was 3 months. After cultivating for 2 days, the branches and leaves of the periwinkle seedlings were harvested, and after drying, vinblastine was extracted and detected by high performance liquid phase method. The vinblastine content was 50.4μg / g DW, which is about 20μg / g DW of vinblastine in common untreated vinca, more than doubled.

Embodiment 3

[0024] H with a concentration of 1000mmol / L 2 o 2 The solution was evenly sprayed on the aerial part of the periwinkle seedlings whose seedling age was 3 months. After cultivating for 2 days, the branches and leaves of the periwinkle seedlings were harvested, and after drying, vinblastine was extracted and detected by high performance liquid phase method. The vinblastine content was 30.7μg / g DW, which is about 20μg / g DW compared with the content of vinblastine in untreated vinca, which is increased, but compared with 100mmol / L, the content increase is less, and the 100mmol / L H 2 o 2 The solution is the optimum concentration.

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Abstract

The invention relates to a plant culture method and particularly relates to a culture method capable of improving the content of vinblastine in a catharanthus roseus plant body. H2O2, ethephon, acetylsalicylic acid and tryptophan are externally applied to catharanthus roseus seedlings or mature plants at certain concentration and sprayed for once or multiple times. branches and leaves of catharanthus roseus are harvested after being treated, the vinblastine in the branches and leaves is extracted and the content is detected, wherein the content can reach 38.2mug / g DW or over through detection, the detected highest content is 62.8mug / g DW, and the content of the vinblastine is improved in comparison with that in common untreated catharanthus roseus. The method is simple and feasible in operation, obvious in effect, the defect that the vinblastine cannot grow through tissue culture is overcome, and a new way is opened up for extraction and utilization of effective ingredients in a plant.

Description

technical field [0001] The invention relates to a plant cultivation method, in particular to a cultivation method for increasing the vinblastine content in periwinkle plants. Background technique [0002] Vinblastine (vinblastine), also known as vinblastine, is an alkaloid proposed from Catharanthus roseus, a plant of the family Apocynaceae, and is a cell cycle-specific antineoplastic drug. It acts on G1, S and M phases, and has a delaying effect on M phases. It can interfere with the formation of the spindle of proliferating cells and stop mitosis in metaphase. And have immunosuppressive effect. It has good curative effect on Hodgkins lymphoma and choriocarcinoma, and has certain curative effect on acute leukemia, breast cancer, ovarian cancer, testicular cancer, head and neck cancer, oropharyngeal cancer and monocytic leukemia . It is also effective for lymphosarcoma, reticulocyte sarcoma, Wilms tumor, and malignant melanoma. [0003] [0004] At present, vinblasti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G13/00
CPCA01G13/00A01G22/00
Inventor 唐中华刘佳王宇刘洋王洪政张衷华
Owner NORTHEAST FORESTRY UNIVERSITY
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